Analysis of the development and function of IL-27-producing T cells
| Project/Area Number |
16K08842
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
三宅 靖延 佐賀大学, 医学部, 准教授 (10392143)
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| Research Collaborator |
YUI Katsuyuki
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | IL-27 / 免疫抑制 / サイトカイン / 感染 / 免疫制御 / 制御性T細胞 / T細胞 / マラリア / 感染症 / シグナル伝達 / 生体分子 |
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| Outline of Final Research Achievements |
Our purpose was to establish IL-27-Venus reporter mice, in which the development of IL-27-producing T cells (Tr27) was to be examined. The reporter mice functioned as designed and we finished backcrossing of the mice into C57BL/6 background.
We previously reported the development of Tr27 in malaria-infected mice. In our preliminary study, we identified IL-27-producing T cell population in the infected mice, albeit at a very low percentage. We thus need to examine the development of Tr27 and its condition by looking at the time course, with enough numbers of fully-backcrossed mice. Although IL-27-producing T cell population in Tuberculosis patients was reported, Tr27 has not been identified in BCG-infected mice. T cells stimulated in vitro by a conventional way did not exhibit IL-27-Venus signal, either. We are planning to further examine the development of Tr27 in various infection models as well as in autoimmune and in inflammatory disease models in fully-backcrossed mice.
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| Academic Significance and Societal Importance of the Research Achievements |
IL-27は免疫抑制作用を持つサイトカインである。IL-27は刺激を受けたマクロファージなどから産生されるとされていたが、我々はマラリア原虫感染マウスにおいてT細胞がIL-27を産生することを報告した。このことは、免疫抑制が生じるべき部位や時間経過を考えるとき、細胞の増殖や遊走の観点から興味深い知見である。また、IL-27の免疫抑制作用は、新規免疫抑制薬・免疫抑制療法の標的として有望であることに加え、新しいチェックポイント分子としての治療応用も注目されており、その作用の詳細の解析は、さまざまな免疫疾患や癌治療を考える上で重要なテーマである。
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Essential roles of C-type lectin Mincle in induction of neuropathic pain in mice.2019
Author(s)
Asako Ishikawa, Yasunobu Miyake, Kimiko Kobayashi, Yuzo Murata, Sayaka Iizasa, Ei’ichi Iizasa, Sho Yamasaki, Naomi Hirakawa, Hiromitsu Hara, Hiroki Yoshida, Toshiharu Yasaka
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Journal Title
Scientific Reports
Volume: 9
Issue: 1
Pages: 872-872
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Apaf1 plays a negative regulatory role in T cell responses by suppressing activation of antigen-stimulated T cells.2018
Author(s)
Tong, H., Miyake, Y., Mi-Ichi, F., Iwakura, Y., Hara, H. and Yoshida, H.
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Journal Title
PLoS One
Volume: 13
Issue: 3
Pages: 0195119-0195119
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Interleukin-27 controls basal pain threshold in physiological and pathological conditions.2018
Author(s)
Tomoko Sasaguri, Toru Taguchi, Yuzo Murata, Kimiko Kobayashi, Sayaka Iizasa, Ei’ichi Iizasa, Makoto Tsuda, Naomi Hirakawa, Hiromitsu Hara, Hiroki Yoshida, Toshiharu Yasaka
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 11022-11022
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] IL-17A contributes to reducing IFN-gamma/IL-4 ratio and persistence of Entamoeba histolytica during intestinal amebiasis2017
Author(s)
Deloer, S. Nakamura, R. Kikuchi, M. Moriyasu, T. Kalenda, Y. D. J. Mohammed, E. S. Senba, M. Iwakura, Y. Yoshida, H. Hamano, S.
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Journal Title
Parasitol Int
Volume: 66
Issue: 6
Pages: 817-823
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The potential for repositioning antithyroid agents as antiasthma drugs.2016
Author(s)
Suzuki S, Ogawa M, Ohta S, Arima K, Nunomura S, Nanri Y, Mitamura Y, Yoshihara T, Nakamura Y, Yamauchi K, Chibana K, Ishii Y, Lee JJ, Aratani Y, Kakuta S, Kubo S, Iwakura Y, Yoshida H, Izuhara K.
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Journal Title
J Allergy Clin Immunol.
Volume: 138
Issue: 5
Pages: 1458-1461
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Possible contribution of the C-type lectin receptor, Mincle, to induction of neuropathic pain after peripheral nerve injury in mice2016
Author(s)
Ishikawa, A Yasaka, T Miyake, Y Murata, Y Sasaguri, T Yamasaki, S Hara, H Yoshida, H Hirakawa, H
Organizer
第16回世界疼痛学会
Place of Presentation
横浜
Year and Date
2016-09-26
Related Report
Invited
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[Book] 免疫学コア講義2017
Author(s)
熊ノ郷 淳、阪口 薫雄、竹田 潔、吉田 裕樹
Total Pages
309
Publisher
南山堂
ISBN
9784525167547
Related Report
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