A circulating microRNA signature as noninvasive biomarkers for NAFLD

• Project/Area Number: 16K08946
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Fujita Health University
• Principal Investigator: Teradaira Ryoji 藤田医科大学, 医療科学部, 名誉教授 (80087668)
• Co-Investigator(Kenkyū-buntansha): 大橋 鉱二 藤田医科大学, 保健学研究科, 教授 (60278281)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: microRNA / NAFLD / バイオマーカー / マイクロRNA(miRNA) / 非アルコール性脂肪肝(NAFLD) / スクリーニング検査法 / マイクロRNA（miRNA) / 非アルコール性脂肪肝（NAFLD） / マイクロRNA（miRNA）

Outline of Final Research Achievements

Non-alcoholic fatty liver disease (NAFLD) is closely associated with obesity, metabolic syndrome, and type II diabetes mellitus. Recently, circulating microRNAs (miRNAs) have been proposed as useful disease biomarkers. We examined whether circulating miRNAs, such as miR-20a, miR-27a, and miR-122, were useful biomarkers for NAFLD. We conducted a cross-sectional analysis of 527 subjects aged 39 years or older who had undergone a health examination in the general people. Serum miR-20a, miR-27a, and miR-122 levels were measured by quantitative real-time PCR. Serum levels of miR-20a and miR-122a were significantly associated with NAFLD subjects. Serum miR-27a levels were significantly lower NAFLD subjects. on the other hands, miR-122 level were significantly higher NAFLD subjects. In conclusion, circulating miR-27a and miR-122 levels were significantly associated with NAFLD subjects. Conbination of circulating miR-27a and miR-122 may be useful biomarkers for NAFLD.

Academic Significance and Societal Importance of the Research Achievements

近年、日本では非アルコール性脂肪肝（NAFLD）になる予備軍が１０００万人いると言われており、早期発見が強く望まれている。NAFLDの診断は，軽度の脂肪化例ではその感度は決して高くなく，ALT値も正常値を示す例があり，NAFLDの拾い上げに有用なパラメータの確立が必要である。　本研究では、血中miRNAを利用することにより、NAFLDをスクリーニングする検査法を開発した。

Research Products

• [Journal Article] Stability of serum high-density lipoprotein-microRNAs for preanalytical conditions. (2017)
  • Author(s): Ishikawa H, Yamada H, Taromaru N, Kondo K, Nagura A, Yamazaki M, Ando Y, Munetsuna E, Suzuki K, Ohashi K, Teradaira R.
  • Journal Title: Ann Clin Biochem Volume: 54 Issue: 1 Pages: 134-142
  • DOI: 10.1177/0004563216647086
  • Peer Reviewed / Open Access
• [Presentation] 非アルコール性脂肪性肝疾患における新規バイオマーカーとしての血清miR-122/miR-27a比の検討 (2017)
  • Author(s): 山崎未来、山田宏哉、宗綱栄二、安藤嘉崇、石川浩章、寺平良治、大橋鉱二
  • Organizer: 第57回日本臨床化学会年次学術集会、北海道大学、札幌市
• [Presentation] The Establishment of Simpler Method for Measuring HDL-MiRNAs (2016)
  • Author(s): Kanako Kondo, Hiroaki Ishikawa, Hiroya Yamada, Mirai Yamazaki, Yuri Murase, Genki Mizuno, Takeru Ota, Eiji Munetsuna, Koji Suzuki, Ryoji Teradaira, Koji Ohashi
  • Organizer: 14th Asia-Pacific Federation for Clinical Biochemistry and Laboratory Medicine Congress
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2016-11-26
  • Int'l Joint Research
• [Presentation] Maternal Fructose Consumption Alters Gene Expression Involved in Hepatic Glucocorticoid Metabolism in Rat Offspring (2016)
  • Author(s): Yuri Murase, Eiji Munetsuna, Hiroya Yamada, Mirai Yamazaki, Genki Mizuno, Kanako Kondo, Takeru Ota, Hiroaki Ishikawa, Ryoji Teradaira, Koji Suzuki, Koji Ohashi
  • Organizer: 14th Asia-Pacific Federation for Clinical Biochemistry and Laboratory Medicine Congress
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2016-11-26
  • Int'l Joint Research
• [Presentation] High Fructose Consumption Induces Hypomethylation of Hepatic Mitochondrial DNA in Rats (2016)
  • Author(s): Mirai Yamazaki, Eiji Munetsuna, Hiroya Yamada, Yoshitaka Ando, Genki Mizuno, Yuri Murase, Kanako Kondo, Takeru Ota, Hiroaki Ishikawa, Ryoji Teradaira, Koji Suzuki, Koji Ohashi
  • Organizer: 14th Asia-Pacific Federation for Clinical Biochemistry and Laboratory Medicine Congress
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2016-11-26
  • Int'l Joint Research