Development of the detection method of curculating tumour cells (CTCs) which act as a sensitive marker for colorectal cancer using new microfluidic CTC chip

• Project/Area Number: 16K08974
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Juntendo University
• Principal Investigator: Tomiki Yuichi 順天堂大学, 医学(系)研究科(研究院), 教授 (40245725)
• Co-Investigator(Kenkyū-buntansha): 小見山 博光 順天堂大学, 医学部, 非常勤講師 (30348982) ; 大永 崇 富山県産業技術研究開発センター, その他部局等, 副主幹研究員 (10416133)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 血中循環腫瘍細胞 / 大腸癌 / マイクロ流体チップ / リキッドバイオプシー / 腫瘍マーカー / 抗EpCAM抗体 / 転移マーカー / 大腸がん / 血中循環癌細胞 / 治療効果判定因子 / サイズ分画CTCチップ / 癌 / 遺伝学 / トランスレーショナルリサーチ

Outline of Final Research Achievements

We developed a CTC detection system to diagnose colorectal cancer (CRC) using blood samples. The system uses the polymeric CTC-chip, a new device for CTC isolation. The high efficiency of　CTC　chip was assessed with cell suspensions of the CRC cell line. The CTC-chip was used to detect CTCs in blood samples obtained from 13 cases of stage II-IV CRS. On average, the number CTCs/ml were lower in patients with stages II and III CRC than in those with stage IV. In cases with stages II-IV, 92% had CTC per ml, which was higher than the positive rate by the carbo- hydrate antigen 19-9 test (CA19-9). Moreover, CTCs were detected in all cases with stage II and III CRC, including cases with negative results for the carcinoembryonic antigen (CEA) and CA19-9 tests. With the CTC-chip detection system, CTCs can be effective cancer markers, particularly for patients in stage II and III CRC who often exhibit negative conventional serum marker test results.

Academic Significance and Societal Importance of the Research Achievements

大腸癌は早期診断により治療効果が高まること、また再発発生の場合も早期対応にて最適治療を行うことができる。今回開発したＣＴＣチップで大腸癌の血中腫瘍細胞を捕捉・同定・計測する方法により、CTCが鋭敏な腫瘍マーカーとして機能し、大腸癌の採血検体による鋭敏な診断が可能になるであろう。またＣＴＣ濃度検出によって、癌の進行の検出を容易にすることからも、臨床上の意義は大きい。

Research Products

• [Journal Article] Using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer (2020)
  • Author(s): Kure K, Hosoya M, Ueyama T, Fukaya M, Sugimoto K, Tomiki Y,Ohnaga T, Sakamoto K, Komiyama H.
  • Journal Title: Oncl Lett Volume: 19 Pages: 2286-2294
  • DOI: 10.3892/ol.2020.11335
  • Peer Reviewed / Open Access
• [Journal Article] Angiopoietin-2 as a prognostic factor in patients with incurable stage IV colorectal cancer. (2020)
  • Author(s): Munakata S, Ueyama T, Ishihara H, Komiyama H, Tsukamoto R, Kawai M, Takahashi M, Kojima Y, Tomiki Y,
  • Journal Title: J Gastrointest Cancer. Volume: 2020 Online ahead of print Issue: 1 Pages: 000-000
  • DOI: 10.1007/s12029-020-00392-1
  • Peer Reviewed / Open Access
• [Journal Article] Experience in the use of a device to attach an anti-adhesion-absorption barrier film to the abdominal cavity in laparoscopic colorectal surgery. (2020)
  • Author(s): Kojima Y, Sakamoto K, Tomiki Y, Mizukoshi K.
  • Journal Title: J Minim Access Surg Volume: 2020 (Ahead Of Print) Issue: 4 Pages: 1-2
  • DOI: 10.4103/jmas.jmas_226_19
  • Peer Reviewed / Open Access
• [Journal Article] Metastatic seeding of human colon cancer cell clusters expressing the hybrid epithelial/mesenchymal state. (2019)
  • Author(s): Mizukoshi K, Okazawa Y, Haeno H, Koyama Y, Sulidan K, Komiyama H, Saeki H, Ohtsuji N, Ito Y, Kojima Y, Goto M, Habu S, Hino O, Sakamoto K, Orimo A.
  • Journal Title: Int J Cancer Volume: 146 Issue: 9 Pages: 2547-2562
  • DOI: 10.1002/ijc.32672
  • Peer Reviewed / Open Access
• [Journal Article] Current status of venous thromboembolism development during the perioperative period for colorectal cancer, its prevention with enoxaparin, and monitoring methods. (2019)
  • Author(s): Aoki J, Sakamoto K, Takahashi R, Niwa K, Ishiyama S, Sugimoto K, Kamiyama H, Takahashi M, Kojima Y, Goto M, Tomiki Y, Iba T.
  • Journal Title: Ther Clin Risk Manag Volume: 15 Pages: 791-802
  • DOI: 10.2147/tcrm.s201954
  • Peer Reviewed / Open Access
• [Journal Article] Significance of serum p53 antibody as a tumor marker in colorectal cancer. (2019)
  • Author(s): Takahashi R, Sakamoto K, Sugimoto K, Motegi S, Tsukamoto R, Ichikawa R, Okazawa Y, Aoki J, Ishiyama S, Takahashi M, Kojima Y, Okuzawa A, Tomiki Y, Matsuoka J.
  • Journal Title: Dis Markers Volume: 2019:2721876 Pages: 1-6
  • DOI: 10.1155/2019/2721876
  • Peer Reviewed / Open Access
• [Journal Article] Effects of receptor for advanced glycation end-products (RAGE) signaling on intestinal ischemic damage in mice. (2019)
  • Author(s): Makino Y, Munakata S, Ueyama T, Honjo H, Kawano S, Takahashi M, Kojima Y, Tomiki T, Sakamoto K.
  • Journal Title: Eur Surg Res Volume: 60(5-6) Issue: 5-6 Pages: 239-247
  • DOI: 10.1159/000504751
  • Peer Reviewed / Open Access
• [Journal Article] Effectiveness of endoscopic sclerotherapy using aluminum potassium sulfate and tannic acid (ALTA) as non-surgical treatment for internal hemorrhoids. (2019)
  • Author(s): Tomiki Y, Aoki J, Motegi S, Takahashi R, Hagiwara T, Okazawa Y, Mizukoshi K, Kawai M, Munakata S, Ishiyama S, Sugimoto K, Sakamoto K.
  • Journal Title: Clin Endosc Volume: 52(6) Issue: 6 Pages: 581-527
  • DOI: 10.5946/ce.2019.017
  • Peer Reviewed / Open Access
• [Journal Article] Study on the efficacy of Daikenchuto on bowel motility after laparoscopic resection of colorectal cancer. (2019)
  • Author(s): Kojima Y, Sakamoto K, Tomiki Y, Nagakari K, Okuzawa A, Takahashi M, Kamiyama H, Ishiyama S, Sugimoto K, Munakata S, Kawai M, Mizukosi K, Okazawa Y.
  • Journal Title: World J Surg Surg Res Volume: 2:1128 Pages: 1-6
  • Peer Reviewed / Open Access
• [Journal Article] Colorectal perforation in patients with connective tissue disease. (2019)
  • Author(s): Sugimoto K, Sakamoto K, Okazawa Y, Takahashi R, Mizukoshi K, Ro H, Kawai M, Kawano S, Munakata S, Ishiyama S, Kamiyama H, Takahashi M, Kojima Y, Tomiki Y, Tamura N.
  • Journal Title: Emerg Med Int Volume: 2019: 5852438. Pages: 1-9
  • DOI: 10.1155/2019/5852438
  • Peer Reviewed / Open Access
• [Journal Article] Serum oxidative stress is an independent prognostic marker in colorectal cancer. (2019)
  • Author(s): Sugimoto K, Sakamoto K, Kawai M, Kawano S, Munakata S, Ishiyama S, Takahashi M, Kojima Y and Tomiki Y.
  • Journal Title: Transl Cancer Res Volume: 8(5) Issue: 5 Pages: 1699-1708
  • DOI: 10.21037/tcr.2019.08.15
  • Peer Reviewed / Open Access
• [Journal Article] Detection of circulating tumor cells with a novel microfluidic system in malignant pleural mesothelioma (2019)
  • Author(s): Yoneda K, Kuwata T, Chikaishi Y, Mori M, Kanayama M, Takenaka M, Oka S, Hirai A, Imanishi N, Kuroda K, Ichiki Y, Ohnaga T, Tanaka F.
  • Journal Title: Cancer Science Volume: 110 Issue: 2 Pages: 726-733
  • DOI: 10.1111/cas.13895
  • Peer Reviewed / Open Access
• [Journal Article] Initial detection of circulating tumor cells from metastatic prostate cancer patients with a novel small device. (2019)
  • Author(s): Obayashi K, Akatsuka J, Endo Y, Takeda H, Hayashi T, Toyama Y, Suzuki Y, Hamasaki T, Kimura G, Ohnaga T, Kondo Y.
  • Journal Title: Prostate Int Volume: 7 Issue: 4 Pages: 131-138
  • DOI: 10.1016/j.prnil.2019.01.003
  • Peer Reviewed / Open Access
• [Journal Article] Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement (2019)
  • Author(s): REI ISHIBASHI, SHUNTARO YOSHIDA, NARIAKI ODAWARA, TAKAHIRO KISHIKAWA, RYO KONDO, AYAKO NAKADA, RYUNOSUKE HAKUTA, NAMINATSU TAKAHARA, ERI TANAKA, KAZUMA SEKIBA, TAKAHIRO SEIMIYA, TAKASHI OHNAGA, MOTOYUKI OTSUKA and KAZUHIKO KOIKE
  • Journal Title: ONCOLOGY LETTERS Volume: 18 Pages: 6397-6404
  • DOI: 10.3892/ol.2019.11047
  • Peer Reviewed / Open Access
• [Journal Article] Neutrophil-lymphocyte ratio as a prognostic factor in incurable stage IV colorectal cancer. (2018)
  • Author(s): Munakata S, Sugimoto K, Honjo K, Kawai M, Kawano S, Kamiyama H, Ouchi M, Takahashi M, Tomiki Y, Sakamoto K.
  • Journal Title: Open Journal of Gastroenterology Volume: 8(02) Issue: 02 Pages: 45-56
  • DOI: 10.4236/ojgas.2018.82005
  • Peer Reviewed / Open Access
• [Journal Article] Repeated laparoscopic resection of extra-regional lymph node metastasis after laparoscopic radical resection for rectal cancer. (2018)
  • Author(s): Sakamoto K, Takahashi M, Takahashi R, Kawano S, Kawai M, Sugimoto K, Kamiyama H, Kojima Y, Okuzawa A, Tomiki Y
  • Journal Title: Journal of minimal access surgery, 14(2), 146. Volume: 24(2) Issue: 2 Pages: 164-168
  • DOI: 10.4103/jmas.jmas_177_17
  • Peer Reviewed / Open Access
• [Journal Article] Capture of mesothelioma cells with 'universal' CTC-chip. (2018)
  • Author(s): Yoneda K, Chikaishi Y, Kuwata T, Ohnaga T, Tanaka F
  • Journal Title: Oncology Letters Volume: 5(2) Pages: 2635-2640
  • DOI: 10.3892/ol.2017.7619
  • Peer Reviewed / Open Access
• [Journal Article] Pharmacological targeting of neutrophil serine proteases prevents lethality in dextran sulfate sodium (DSS)-induced colitis in mice (2018)
  • Author(s): Ro H, Munakata S, Ueyama T, Komiyama H, Takahashi M, Kojima Y, Tomiki Y, Sakamoto K
  • Journal Title: Journal of Gastrointestinal & Digestive System Volume: 8 Issue: 01 Pages: 551-551
  • DOI: 10.4172/2161-069x.1000551
  • Peer Reviewed / Open Access
• [Journal Article] High-sensitivity detection of micrometastases generated by gfp lentivirus-transduced organoids cultured from a patient-derived colon tumor (2018)
  • Author(s): Okazawa Y, Mizukoshi K, Koyama Y, Okubo S, Komiyama H, Kojima Y, Goto M, Habu S, Hino O, Sakamoto K, Orimo A.
  • Journal Title: Journal of Visualized Experiments (in press) Volume: -- Pages: 2018-2018
  • Peer Reviewed
• [Journal Article] Survival benefit of lymph node dissection in surgery for colon cancer in elderly patients:multicenter propensity score-matched study in Japan. (2018)
  • Author(s): Takahashi M, Niitsu H, Sakamoto K, Hinoi T, Hattori M, Goto M, Bando H, Hazama S, Maeda K, Okita K, Horie H, Watanabe M; Japan Society of Laparoscopic Colorectal Surgery.
  • Journal Title: Asian J Endosc Surg Volume: --
  • Peer Reviewed
• [Journal Article] Salivary Chromogranin A as a Psychosomatic Stress Marker Is Suppressed in Laparoscopic Surgery Compared with Open Surgery for Colon Cancer (2017)
  • Author(s): Ishiyama S, Sakamoto K, Kamiyama H, Niwa K, Sugimoto K, Takahashi M, KojimaY, Goto M, Okuzawa A, Tomiki Y:
  • Journal Title: Juntendo Medical Journal Volume: 63 Issue: 2 Pages: 88-94
  • DOI: 10.14789/jmj.63.88
  • NAID: 130006944480
  • ISSN: 2187-9737, 2188-2126
  • Peer Reviewed / Open Access
• [Journal Article] EpCAM-independent capture of circulating tumor cells with a 'universal CTC-chip' (2017)
  • Author(s): Chikaishi Y, Yoneda K, Ohnaga T, Tanaka F
  • Journal Title: ONCOLOGY REPORTS Volume: 37.1 Issue: 1 Pages: 77-82
  • DOI: 10.3892/or.2016.5235
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Salivary Chromogranin A as a Psychosomatic Stress Marker is suppressed in Laparoscopic Surgery compared with Open Surgery for Colon Cancer. (2017)
  • Author(s): Ishiyama S, Sakamoto K, Kamiyama H, Niwa K, Sugimoto K, Takahashi M, KojimaY, Goto M, Okuzawa A, Tomiki Y
  • Journal Title: Juntendo Medical Journal Volume: 63(2) Pages: 88-94
  • NAID: 130006944480
  • Peer Reviewed / Open Access
• [Journal Article] Minimally Invasive Surgery for Colorectal Cancer (2017)
  • Author(s): Sakamoto K, Tsukamoto R, Kawano S, Kawai M, Niwa K, Ishiyama S, Sugimoto K, Kamiyama H, Komiyama H, Takahashi M, Kojima Y, Goto M, Okuzawa A, Tomiki Y:
  • Journal Title: Juntendo Medical Journal Volume: 63 Issue: 5 Pages: 384-392
  • DOI: 10.14789/jmj.63.384
  • NAID: 130006219695
  • ISSN: 2187-9737, 2188-2126
  • Peer Reviewed / Open Access
• [Journal Article] Salivary chromogranin a as a psychosomatic stress marker is suppressed in (2017)
  • Author(s): Ishiyama S, Sakamoto K, Kamiyama H, Niwa K, Sugimoto K, Takahashi M, Kojima Y, Goto M, Okuzawa A, Tomiki Y
  • Journal Title: Juntendo Medical Journal Volume: 63(2) Pages: 1-7
  • Peer Reviewed / Open Access
• [Journal Article] An impact of Chromosome 17q deletion in the primary lesion of colorectal cancer on liver metastasis. (2016)
  • Author(s): Kawai M, Komiyama H, Hosoya M, Okubo H, Fujii T, Yokoyama N, Sato C, Ueyama T, Okuzawa A, Goto M, Kojima Y, Takahashi M, Sugimoto K, Ishiyama S, Munakata S, Ogura D, Niwa S, Tomiki Y, Ochiai T, Sakamoto K:
  • Journal Title: Oncology Letters Volume: 12(6) Issue: 6 Pages: 4773-4778
  • DOI: 10.3892/ol.2016.5271
  • Peer Reviewed / Open Access
• [Journal Article] Postoperative Adjuvant Chemotherapy Improves Survival in Stage II Colon Cancer ? A Propensity Score Matching Analysis (2016)
  • Author(s): Sugimoto K, Sakamoto K, Niwa K, Ishiyama S Kamiyama H, Komiyama H, Takahashi M,Kojima Y, Goto M, Tomiki Y:
  • Journal Title: Juntendo Medical Journal Volume: 62 Issue: 6 Pages: 463-471
  • DOI: 10.14789/jmj.62.463
  • NAID: 130005402125
  • ISSN: 2187-9737, 2188-2126
  • Peer Reviewed / Open Access
• [Journal Article] The Analysis of Prognosis by Data Mining Using the Decision Tree Analysis in Stage Ⅲ Colon and Rectosigmoid Cancer (2016)
  • Author(s): 杉本 起一・丹羽浩一郎・石山 隼 神山 博彦・小見山博光・高橋 玄・小島 豊・五藤 倫敏・冨木 裕一・坂本 一博
  • Journal Title: Nihon Gekakei Rengo Gakkaishi (Journal of Japanese College of Surgeons) Volume: 41 Issue: 1 Pages: 1-7
  • DOI: 10.4030/jjcs.41.1
  • NAID: 130005397845
  • ISSN: 0385-7883, 1882-9112
  • Peer Reviewed / Open Access
• [Journal Article] Capture of esophageal and breast cancer cells with polymeric microfluidic devices for CTC isolation (2016)
  • Author(s): TAKASHI OHNAGA, YUTAKA SHIMADA, KOJI TAKATA, TSUTOMU OBATA, TOMOYUKI OKUMURA, TAKUYA NAGATA, HIROYUKI KISHI, ATSUSHI MURAGUCHI and KAZUHIRO TSUKADA
  • Journal Title: MOLECULAR AND CLINICAL ONCOLOGY Volume: 4 Issue: 4 Pages: 599-602
  • DOI: 10.3892/mco.2016.734
  • Peer Reviewed / Open Access
• [Presentation] Temporary diverting stoma at the time of the rectal cancer operation. (2019)
  • Author(s): Kojima Y, Sakamoto K, Tomiki Y, Okuzawa A, Takahashi M, Sugimoto K, Kawai M, Okazawa Y, Mizukoshi K, Makino Y, Amemiya K, Hagiwara T, Fukunaga T.
  • Organizer: The 27th International Congress of the European Association for Endoscopic Surgery, Sevilla, Andalucia.
  • Int'l Joint Research
• [Presentation] A study of complications after robotic surgery for rectal cancer. (2019)
  • Author(s): Tsuchiya Y, Okazawa Y, Mizukoshi K, Kawai M, Sugimoto K, Kamiyama H, Takahashi M, Kojima Y, Tomiki Y, Fukunaga T, Kajiyama Y, Sakamoto K.
  • Organizer: 2019 European Association for Endoscopic Surgery -Spain.
  • Int'l Joint Research
• [Presentation] Circulating plasma dna methylation status of tumor suppressor genes may be a biomarker of lung metastasis in colorectal cancer. (2019)
  • Author(s): Sugimoto K, Amemiya K, Tsuchiya Y, Hagiwara T, Matsuzawa T, Makino Y, Motegi S, Tsukamoto R, Kawai M, Kawano S, Niwa K, Ishiyama S, Kamiyama H, Takahashi M, Kojima Y, Tomiki Y, Fukunaga T, Kajiyama Y, Kawasaki S, Sakamoto K.
  • Organizer: Digestive Disease Week 2019, San Diego, USA.
  • Int'l Joint Research
• [Presentation] 網羅的解析を用いた新規結腸癌診断マーカーの探索. (2019)
  • Author(s): 塚本亮一, 小見山博光, 河野眞吾, 河合雅也, 高橋玄, 小島豊, 冨木裕一, 坂本一博:
  • Organizer: 第57回日本癌治療学会学術集会, 福岡.
• [Presentation] ポリマー製マイクロ流体チップを用いた大腸癌患者における CTC の測定方法 と現状. (2018)
  • Author(s): 土谷祐樹, 呉 一眞, 細谷理樹, 深谷 緑, 植山孝恵, 河野眞吾, 杉本起一, 小見山博光, 高橋 玄, 小島 豊, 冨木裕一, 大永 崇, 坂本一博:
  • Organizer: 第２回 Liquid Biopsy 研究会. 東京
• [Presentation] Semaphorin 3C has a possibility of a new prognostic marker in colorectal cancer. (2017)
  • Author(s): Kawano S, Komiyama H, Tomiki Y, Sakamoto K et al.
  • Organizer: 110th AACR, Washington DC.
  • Int'l Joint Research
• [Presentation] Identification of a novel diagnostic marker for colon cancer through comprehensive microRNA analysis. (2017)
  • Author(s): Tsukamoto R, Komiyama H, Hosoya M, Kure K, Okazawa Y, Mizukoshi K, Fukaya M, Sugimoto K, Kamiyama H, Tomiki Y, Sakamoto K
  • Organizer: 第76回日本癌学会学術総会 横浜
• [Presentation] Postoperative adjuvant chemotherapy improves survival in stage II colon cancer ? a propensity score matching analysis. (2017)
  • Author(s): Tsukamoto R, Matsuzawa M, Hagiwara T, Tsuchiya Y, Amemiya K, Motegi S, Makino Y, Kawano S, Honjo K, Kure K, Ichikawa R, Ro H, Ito S, Takahashi R, Niwa K, Ishiyama S, Sugimoto K, Kamiyama H, Takahashi M, Kojima Y, Goto M, Okuzawa A, Tomiki Y, Fukunaga T, Kajiyama Y, Kawasaki S, Sakamoto K
  • Organizer: 110th AACR, Washington DC
  • Int'l Joint Research
• [Presentation] The prognostic capability of oxidative stress after surgery in colorectal cancer. (2017)
  • Author(s): Sugimoto K, Tsuchiya H, Hagiwara T, Matsuzawa H, Makino Y, Motegi S, Tsukamoto R, Kawai M, Kawano S, Niwa K, Ishiyama S, Kamiyama H, Takahashi M, Kojima Y, Tomiki Y, Sakamoto K
  • Organizer: The 21th Asian Congress of Surgery, Tokyo
  • Int'l Joint Research
• [Presentation] Production of monoclonal antibodies against amino acid transporter SLC7A1 to develop novel therapy for colorectal cancer. (2017)
  • Author(s): Fukaya M, Komiyama H, Sugimoto K, Kamiyama H, Sakamoto K:
  • Organizer: 第76回日本癌学会学術総会, 横浜
• [Presentation] ポリマー製マイクロ流体チップを用いた大腸癌患者におけるCTCの測定 (2016)
  • Author(s): 呉一眞， 細谷理樹， 深谷緑 ， 植山孝恵， 丹羽浩一郎， 石山隼， 神山博彦， 小見山博光 ， 高橋玄， 五藤倫敏， 奥澤淳司， 冨木裕一， 大永崇， 坂本一博
  • Organizer: 第54回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2016-10-20
• [Presentation] Multicellular tumor clusters seed metastases in colon patientderived tumor xenografts (2016)
  • Author(s): Okubo S, Mizukoshi K, Okazawa Y, Haeno H, Komiyama H, Saeki H, Ito Y, Gotou M Watanabe S, Hino O, Sakamoto K, Orimo A
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2016-10-07
• [Presentation] 残胃癌におけるゲノムワイドDNAメチル化解析 (2016)
  • Author(s): 杉本起一，伊藤智彰，折田創，櫛田知志，桜田睦，前川博，小見山博光，高橋玄，冨木裕一，坂本一博，佐藤浩一
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2016-10-06
• [Presentation] Polymeric CTC-chipを用いた大腸癌患者に対するCTCの検出 (2016)
  • Author(s): 呉 一眞，小見山 博光, 細谷 理樹:, 植山 孝恵, 大久保 はるな, 深谷 緑, 土谷 祐樹, 塚本 亮一, 伊藤 慎吾, 市川 亮介, 本庄 薫平, 盧 尚志, 河野 眞吾, 杉本 起一, 神山 博彦, 高橋 玄, 奥澤 淳司, 冨木 裕一, 大永 崇, 坂本 一博
  • Organizer: 第117回日本外科学会定期学術集会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2016-04-14
• [Remarks] 消化器外科学講座（下部消化管外科学）
  • URL: https://www.juntendo.ac.jp/med/labo/kabusyoukakan.html
• [Remarks] 順天堂大学 下部消化管外科
  • URL: https://www.juntendo.ac.jp/graduate/laboratory/labo/kabusyoukakan/k6.html
• [Remarks] 順天堂大学大学院 下部消化管外科学 （教室トップページ）
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/kabusyoukakan/
• [Remarks] 順天堂大学付属順天堂病院 大腸・肛門外科
  • URL: https://www.juntendo.ac.jp/hospital/clinic/daicho/