Establishment of non-invasive evaluation method of multistage liver carcinogenesis model using bioimaging technology

• Project/Area Number: 16K09017
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical Physics and Radiological Technology
• Research Institution: Osaka University
• Principal Investigator: Saito Shigeyoshi 大阪大学, 医学系研究科, 助教 (40583068)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 生体イメージング / 多段階発がんモデル / MRI / マイクロCT / 肝多段階発がんモデル / 肝疾患モデル / 慢性肝障害モデル / 肝硬変モデル / 磁気共鳴医学 / 肝がん / Micro-CT

Outline of Final Research Achievements

The purpose of this study was noninvasively and longtudinally to evaluate the liver carcinogenesis model by in vivo imaging technology. We used two MRI equipments: 11.7 T-MRI and 1.5 T-MRI for small animals, to detect and evaluate the longtudinal change in disease state until canceration. In addition, factors related to carcinogenesis and cancer growth were simultaneously evaluated from the aspects of immunohistological staining and blood test. We evaluated and performed using biological imaging technology of a liver multistage carcinogenesis model.

Academic Significance and Societal Importance of the Research Achievements

本研究では生体イメージング技術により肝多段階発がんモデルを経時的かつ非侵襲的に評価した。先行研究では急性や慢性肝炎、肝線維化など肝がんへ移行する前段階での評価や、薬物誘発や腫瘍移植による進行がんモデルや担癌モデルなどの研究はあるが、肝炎発症から慢性肝炎を経てがん化するまでの長期観察が必要な多段階発がんモデルを生体イメージング技術により評価した研究はなく、本試みは学術的な意義が大きい。本研究は、先行研究の知見を補完し、さらに多段階発がんの過程を可視化することにより発がん過程の連続的評価を可能とする新しい試みである。

Research Products

• [Journal Article] Detection of increased intracerebral lactate in a mouse model of Leigh syndrome using proton MR spectroscopy (2019)
  • Author(s): Takahashi Yusuke、Kioka Hidetaka、Shintani Yasunori、Ohki Akiko、Takashima Seiji、Sakata Yasushi、Higuchi Takahiro、Saito Shigeyoshi
  • Journal Title: Magnetic Resonance Imaging Volume: 58 Pages: 38-43
  • DOI: 10.1016/j.mri.2019.01.010
  • Peer Reviewed / Open Access
• [Journal Article] Early detection of elevated lactate levels in a mitochondrial disease model using chemical exchange saturation transfer (CEST) and magnetic resonance spectroscopy (MRS) at 7T-MRI (2018)
  • Author(s): Saito Shigeyoshi、Takahashi Yusuke、Ohki Akiko、Shintani Yasunori、Higuchi Takahiro
  • Journal Title: Radiological Physics and Technology Volume: 12 Issue: 1 Pages: 46-54
  • DOI: 10.1007/s12194-018-0490-1
  • Peer Reviewed
• [Journal Article] Prenatal Irradiation-Induced Hippocampal Abnormalities in Rats Evaluated Using Manganese-Enhanced MRI (2018)
  • Author(s): Saito Shigeyoshi、Sawada Kazuhiko、Aoki Ichio
  • Journal Title: Frontiers in Neural Circuits Volume: 12 Pages: 1-7
  • DOI: 10.3389/fncir.2018.00112
  • Peer Reviewed
• [Journal Article] Longitudinal observations of cardiomyopathy model hamster using high-filed 11.7T-MRI (2017)
  • Author(s): 1.Shigeyoshi Saito*, Minori Tanoue, Kasumi Masuda, Yuki Mori, Satoshi Nakatani, Yoshichika Yoshioka and Kenya Murase.
  • Journal Title: Scientific Reports Volume: ７ Pages: 9106-9106
  • Peer Reviewed / Open Access
• [Journal Article] Mapping of left ventricle wall thickness in mice using 11.7-T magnetic resonance imaging (2017)
  • Author(s): 3.Shigeyoshi Saito*, Kasumi Masuda, Yuki Mori, Satoshi Nakatani, Yoshichika Yoshioka and Kenya Murase.
  • Journal Title: Magnetic Resonance Imaging Volume: 36 Pages: 128-134
  • Peer Reviewed