MicroRNAs characteristic to the high incidence area of ALS in the Kii Peninsula
| Project/Area Number |
16K09085
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Epidemiology and preventive medicine
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| Research Institution | Kansai University of Health Sciences |
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Principal Investigator |
Kihira Tameko 関西医療大学, 保健医療学部, 教授 (30225015)
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| Co-Investigator(Kenkyū-buntansha) |
伊東 秀文 和歌山県立医科大学, 医学部, 教授 (20250061)
荒川 裕也 関西医療大学, 保健医療学部, 助教 (30733175)
伊藤 俊治 関西医療大学, 保健医療学部, 准教授 (50275351)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 紀伊ALS / 多発地ALS / 多発地住民 / 血清HEL / 血清亜鉛 / 血清microRNA / 酸化ストレス / Cu/Zn SOD / 血清miRNA / Cu/ZnSOD / 紀伊半島多発地ALS / microRNA / 酸化的ストレス / SOD-1 / HEL / 亜鉛 / miRNA / カルシウム |
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| Outline of Final Research Achievements |
The ALS incidence in the southern area of the Kii Peninsula (K-ALS) was previously higher than other areas, but recently it has been decreased to the average level of the world, suggesting that environmental factors play a role in development of the disease. We studied oxidative stress as environmental factors including serum contents of minerals, HEL (an oxidative marker of lipid) and urinary 8-OHdGc (an oxidative marker of DNA) in patients with K-ALS. We found significantly low contents of Zn, high contents of HEL and 8-OHdGc in patients with K-ALS compared to controls.These results may suggest that the increase of oxidative stress is associated with a risk of development of ALS in this area.
MicroRNAs (miRs) have been reported to play a role in neuro-degeneration. We found 16 candidate miRs related to K-ALS by microarray. Several miRs out of the 16 were significantly higher in the K-ALS compared to controls. We consider that these miRs may play a role in the development of K-ALS.
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| Academic Significance and Societal Importance of the Research Achievements |
我々は、1960年代にALSが多発した紀伊半島南部地域で環境要因とALS発症の関連を検討し、環境中及び住民や患者検体からCaやZn等必須元素の低値と有害元素の高値を認め報告してきた。また生活習慣の改善が最近のALS発症頻度の低下と関連する可能性を報告してきた。
本研究では新たに紀伊のALS患者血清からALSに関連する候補microRNA(多疾患で関与が示唆されている)と脂質の酸化ストレスを示すHEL高値を認めた。本地域では、なんらかの環境要因とALS発症との関連が考えられるが、生活スタイルなど環境要因の変化が発症抑制的に作用するならば、その要因の解明は今後の予防や治療法開発に重要と考える。
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Lifestyle Changes and Oxidative Stress in a High-incidence Area of Amyotrophic Lateral Sclerosis in the Southwestern Kii Peninsula, Japan2017
Author(s)
Kihira T, Okamoto K, Sakurai I, Arakawa Y, Wakayama I, Takamiya K, Okumura R, Iinuma Y, Iwai K, Kokubo Y, Yoshida S
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Journal Title
Internal Medicine
Volume: 56
Issue: 12
Pages: 1497-1506
DOI
NAID
ISSN
0918-2918, 1349-7235
Related Report
Peer Reviewed / Open Access
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