| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We established four types of 5-FU resistant colon cancer cell line in vitro and in vivo. We then analyzed miRNA expression profile by miRNA array in these 4 cell lines, and identified the drug resistance-related miRNAs. We identified possible miRNAs to be involved in 5-FU resistance by miRNA arrays. We then examined the relationship between miR-31, which was most promising among them, and drug resistance. When we exposed high dose of 5-FU to parent or 5-FU resistant cells, the expression levels of miR-31 were raised higher than those of controls. Notably, the expression levels of miR-31 were positively correlated with the grade of clinical stages of colorectal tumors. The protein expression levels of factor inhibiting hypoxia-inducible factor 1 (FHI-1) were downregulated by transfection of mimic miR-31 into DLD-1 cells. This study provides evidence supporting the association of miR-31 with 5-FU drug resistance and clinical stages of colorectal tumors.
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