Elucidation of the mechanisms of atrial fibrillation with the lifestyle diseases, and the attempt for the prediction of atrial fibrillation
Project/Area Number |
16K09494
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cardiovascular medicine
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
SASANO Tetsuo 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00466898)
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Project Period (FY) |
2016-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 心房細動 / 細胞外核酸 / マイクロRNA / バイオマーカー / cell-free DNA / 心房リモデリング / セルフリーDNA / パネキシン / トランスレーショナルリサーチ / 循環器 / 生活習慣病 |
Outline of Final Research Achievements |
Although it is known that metabolic syndrome and hypertension are the risk factor for atrial fibrillation (AF), the thorough mechanism has been still uncovered. We generated the disease model mice, and performed a comprehensive analysis of microRNA in murine atria. We found that miR-27b was increased in murine atrial with a high-fat diet model, which suppressed the expression of connexin-40, resulting in the conduction disturbance in the atria. We also performed a comprehensive analysis of circulating microRNA in patients with AF, and analyzed them in combination with that of murine atria. We finally identified the novel 4 miRs predicting the AF, and established a diagnostic miR panel to predict AF. These results may contribute to the development of precision medicine and preventive therapy for AF.
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Academic Significance and Societal Importance of the Research Achievements |
心房細動は日本で最も患者数の多い頻脈性不整脈であり、患者数は約100万人と報告されている。しかし、心房細動の40%は無症状であることなどの理由により、診断に至っていない患者数も100万人前後いると考えられている。心房細動の最も重篤な合併症は脳梗塞であり、抗凝固薬による予防が有効だが、心房細動の診断にいたらないまま脳梗塞が初発症状となることが問題となっている。本研究では、心房細動の発症メカニズムとしてのマイクロRNAの寄与と、バイオマーカーとしての血中マイクロRNAについて検討し、血液検査による新たな心房細動診断パネルを確立した。今後、心房細動の早期発見・先制医療への応用が期待される。
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Report
(5 results)
Research Products
(53 results)
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[Journal Article] Combined Analysis of Human and Experimental Murine Samples Identified Novel Circulating MicroRNAs as Biomarkers for Atrial Fibrillation2018
Author(s)
Natsume Y, Oaku K, Takahashi K, Nakamura W, Oono A, Hamada S, Yamazoe M, Ihara K, Sasaki T, Goya M, Hirao K, Furukawa T, Sasano T.
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Journal Title
Circulation Journal
Volume: 82
Issue: 4
Pages: 965-973
DOI
NAID
ISSN
1346-9843, 1347-4820
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genetic defects in a His-Purkinje system transcription factor, IRX3, cause lethal cardiac arrhythmias.2016
Author(s)
Koizumi A, Sasano T, Kimura W, Miyamoto Y, Aiba T, Ishikawa T, Nogami A, Fukamizu S, Sakurada H, Takahashi Y, Nakamura H, Ishikura T, Koseki H, Arimura T, Kimura A, Hirao K, Isobe M, Shimizu W, Miura N, Furukawa T.
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Journal Title
European Heart Journal.
Volume: 37 (18)
Issue: 18
Pages: 1469-1475
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Novel dielectric coagulation identifies hypercoagulability in patients with a high CHADS2 score without atrial fibrillation2016
Author(s)
99. Hasegawa Y, Hamada S, Nishimura T, Sasaki T, Ebana Y, Kawabata M, Goya M, Isobe M, Koyama T, Furukawa T, Hirao K, Sasano T
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Journal Title
PLoS ONE
Volume: 11
Issue: 6
Pages: e0156557-e0156557
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Circulating Cell-free DNA and Cell-free Mitochondrial DNA in Patients with Atrial Fibrillation.2018
Author(s)
Takahashi N, Nakamura W, Yamazoe M, Natsume Y, OonoA, Shiohira S, shira Y, Yagishita A, Sasaki T, Goya M, Hirao K, Furuakwa T, Sasano T
Organizer
Asia-pacific Heart Rhythm Society meeting. Taipei, Taiwan
Related Report
Int'l Joint Research
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[Presentation] Elevated Circulating Cell-free DNA in Subjects with Atrial Fibrillation.2017
Author(s)
Nakamura W, Yamazoe M, Natsume Y, Oono A, Shiohira S, Shirai Y, Yagishita A, Sasaki T, Goya M, Hirao K, Furukawa T, Sasano T
Organizer
Asia-pacific Heart Rhythm Society meeting
Related Report
Int'l Joint Research
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[Presentation] valuation of the Effect of Anticoagulants Using Novel Dielectric Coagulometer.2017
Author(s)
Oono A, Hamada S, Hasegawa Y, Shiohira S, Nishimura T, Shirai Y, Yagishita A, Sasaki T, Goya M, Furukawa T, Hirao K, Sasano T
Organizer
Asia-pacific Heart Rhythm Society meeting
Related Report
Int'l Joint Research
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[Presentation] Transient cryo-stimulation increases whole blood coagulability: Potential risk of thrombosis during cryoablation.2017
Author(s)
Sasano T, Suzuki A, Oono A, Nakamura W, Natsume Y, Hasegawa Y, Hamada S, Yamauchi Y, Okishige K, Isobe M, Hirao K
Organizer
第81回日本循環器学会
Place of Presentation
金沢フォーラス(石川県、金沢市)
Related Report
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[Presentation] Individual evaluation of whole blood coagulability and factor Xa activity by novel dielectric blood coagulometry.2016
Author(s)
Sasano T, Hamada S, Hasegawa Y, Nishimura T, Yoshitake T, Shiohira S, Sasaki T, Kawabata M, Goya M, Isobe M, Furukawa T, Hirao K
Organizer
第63回日本不整脈心電学会
Place of Presentation
札幌コンベンションセンター(北海道、札幌市)
Related Report
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