Iron-induced calcification in human aortic vascular smooth muscle cells
Project/Area Number |
16K09661
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Kidney internal medicine
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Research Institution | Hyogo Medical University |
Principal Investigator |
Kida Aritoshi 兵庫医科大学, 医学部, 助教 (30647516)
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Co-Investigator(Kenkyū-buntansha) |
長澤 康行 兵庫医科大学, 医学部, 講師 (10379167)
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Project Period (FY) |
2016-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 石灰化 / 大動脈 / 透析 / 鉄 / IL-24 / 腎性貧血 / メンケベルグ型動脈硬化 / 酸化的ストレス / 血管中膜細胞 / 動脈硬化 |
Outline of Final Research Achievements |
We established cultured human aortic medial cells which can demonstrate the calcification process induced by the stimulation of iron. We evaluated the gene expression comprehensively by microarray. The Microarray analysis detected that the IL-24 gene expression was enhanced by iron stimulation and TGF-beta stimulation. Moreover, we confirmed time course of gene expression change and protein level change. We reported these results as article(Iron-induced calcification in human aortic vascular smooth muscle cells through interleukin-24 (IL-24), with/without TNF-alpha. Kawada S, Nagasawa Y, Kawabe M, Ohyama H, Kida A, Kato-Kogoe N, Nanami M, Hasuike Y, Kuragano T, Kishimoto H, Nakasho K, Nakanishi T. Sci Rep. 2018 Jan 12;8(1):658. doi: 10.1038/s41598-017-19092-1) and international meetings.
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Academic Significance and Societal Importance of the Research Achievements |
透析患者において腎性貧血の管理において鉄の投与は主要な治療法のひとつであるが、同時に酸化的ストレスを増強することなどから、動脈硬化を促進する可能性が従来から指摘されている。慢性腎不全における石灰化の主要な場は血管中膜細胞(メンケベルグ型動脈硬化)である。鉄負荷が血管中膜細胞の石灰化を増強し、そのプロセスにTGF-βに代表される炎症とIL-24が関わることを明らかにした。さらに、このプロセスは抗IL-24抗体で中和されることも見出した。この知見は、新規の動脈硬化抑制の治療法を確立に繋がっていくことが期待される。
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Report
(5 results)
Research Products
(13 results)
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[Journal Article] The association of alcohol and smoking with CKD in a Japanese nationwide cross-sectional survey.2017
Author(s)
Matsumoto A, Nagasawa Y, Yamamoto R, Shinzawa M, Hasuike Y, Kuragano T, Isaka Y, Nakanishi T, Iseki K, Yamagata K, Tsuruya K, Yoshida H, Fujimoto S, Asahi K, Moriyama T, Watanabe T.
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Journal Title
Hypertens Res
Volume: Epub
Issue: 8
Pages: 771-778
DOI
Related Report
Peer Reviewed
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[Journal Article] Serum Uric Acid Level Predicts Progression of IgA Nephropathy in Females but Not in Males2016
Author(s)
Nagasawa Y, Yamamoto R, Shoji T, Shinzawa M, Hasuike Y, Nagatoya K, Yamauchi A, Hayashi T, Kuragano T, Moriyama T, Isaka Y and Nakanishi T
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Journal Title
PLoS One
Volume: EPub
Issue: 8
Pages: e0160828-e0160828
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Body Mass Index Modifies an Association between Self-Reported Regular Exercise and Proteinuria2016
Author(s)
Yasuyuki Nagasawa, Ryouhei Yamamoto, Maki Shinzawa, Yukiko Hasuike, Takahiro Kuragano, Yoshitaka Isaka, Takeshi Nakanishi, Kunitoshi Iseki, Kunihiro Yamagata, Kazuhiko Tsuruya, Hideaki Yoshida, Shouichi Fujimoto, Koichi Asahi, Toshiki Moriyama, Tsuyoshi Watanabe
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Journal Title
Journal of Atherosclerosis and Thrombosis
Volume: 23
Issue: 4
Pages: 402-412
DOI
NAID
ISSN
1340-3478, 1880-3873
Related Report
Peer Reviewed / Open Access
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[Presentation] Iron induced calcification in human aortic vascular smooth muscle cells through interleukin-24 (IL-24)2018
Author(s)
Yasuyuki Nagasawa, Sayuri Kawada, Mutsuki Kawabe, Aritoshi Kida, Mana Yahiro, Masayoshi Nanami, Yukiko Hasuike, Takahiro Kuragano, Keiji Nakasho, Hideki Ohyama,Takeshi Nakanishi
Organizer
55th ERA-EDTA Congress
Related Report
Int'l Joint Research
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[Presentation] IFI27/ ISG12A in peripheral white blood cells is a useful marker for IgA nephropathy2016
Author(s)
Yasuyuki Nagasawa1, Eri Muso, Ryohei Yamamoto, Maki Shinzawa, Yukako Iwasaki, Hirotsugu Iwatani, Takeshi Nakanishi, Yoshitaka Isaka1
Organizer
Annual Meeting of American Society of Nephrology, Nov,19, 2016 Chicago, USA
Place of Presentation
Chicago, USA
Year and Date
2016-11-15
Related Report
Int'l Joint Research