Determination of cellular factors involved in chikungunya virus infection

• Project/Area Number: 16K09934
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Osaka University
• Principal Investigator: Tanaka Atsushi 大阪大学, 微生物病研究所, 特任講師(常勤) (20321953)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: チクングニアウイルス / ゲノムワイド分析 / ゲノムワイドスクリーニング / シュードタイプウイルスベクター / 細胞性因子

Outline of Final Research Achievements

Genome-wide analysis of virus-resistant surviving cells from a gene knockout haploid cell library inoculated with the chikungunya virus (CHIKV) pseudotype suggested the one of membrane protein candidate which related to CHIKV infection. We could clarify that this novel membrane protein is significantly involved in the infection of CHIKV Thai # 16856 clinical isolates. Elucidation of the mechanism of action of this new membrane protein during CHIKV infection would be an important finding for the development of a preventive/therapeutic medicine for CHIKV infection.

Academic Significance and Societal Importance of the Research Achievements

チクングニアウイルスのヒト細胞への結合機構ならびに侵入機構といった一連の感染機構の詳細は明らかにされておらず、またチクングニアウイルスに対する予防薬や治療薬、ワクチンも開発されていないという状況である。本研究ではチクングニアウイルスのヒト細胞への感染増殖機構を明らかにするため、ヒト細胞におけるチクングニアウイルス感染関連細胞性因子の同定を試みた。今回得られたチクングニアウイルス感染関連細胞性因子は将来、チクングニアウイルス感染の予防薬や治療薬の標的となる可能性がある。

Research Products

• [Int'l Joint Research] RCC-ERI/NIH, DMSC, MOPH, Thailand(タイ)
• [Journal Article] The Use of Green Fluorescent Protein-Tagged Virus-Like Particles as a Tracer in the Early Phase of Chikungunya Infection (2019)
  • Author(s): Tumkosit, U., Maeda, Y., Kishishita, N., Siripanyaphinyo, U., Omori, H., Chetanachan, P., Sittisaman, P., Jityam, C., Priengprom, T., Mizushima, H., Wongjaroen, P., Mekada, E., Tatsumi, M., Takeda, N., and Tanaka, A.
  • Journal Title: Virus Research Volume: 272 Pages: 197732-197732
  • DOI: 10.1016/j.virusres.2019.197732
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genome-Wide Screening Uncovers the Significance of N-sulfation of Heparan Sulfate as a Host Cell Factor for Chikungunya Virus Infection (2017)
  • Author(s): Tanaka, A. Tumkosit, U. Nakamura, S. Motooka, D. Kishishita, N. Priengprom, T. Sa-Ngasang, A. Kinoshita, T. Takeda, N. Maeda, Y.
  • Journal Title: Journal of virology Volume: Volume 91(13) Issue: 13
  • DOI: 10.1128/jvi.00432-17
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] The use of green fluorescent protein-tagged virus-like particles as a tracer in the early phase of chikungunya infection. (2019)
  • Author(s): Tumkosit, U. Maeda, Y., Kishishita, N., Tatsumi, M., Takeda, N., and Tanaka, A.
  • Organizer: 第67回日本ウイルス学会学術集会