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Determination of causative drugs using ex-vivo expanded skin infiltrating T cells in drug eruptions

Research Project

Project/Area Number 16K10151
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dermatology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

Fujiyama Toshiharu  浜松医科大学, 医学部附属病院, 講師 (60402301)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords薬疹 / 原因薬剤 / MDR1 / 重症薬疹
Outline of Final Research Achievements

It is not always easy to determine the causative drugs in drug eruption cases. We usually perform DLST, a conventional method to determine the causative drug using peripheral blood mononuclear cells. Suspicious drugs are added to the cells to measure the uptake of 3H-thymidine. However, the detection rate is not satisfactory. Therefor we tried to establish new methods to determine the causative drugs by using ex-vivo expanded skin infiltrating T cells.
We successfully determine the causative drugs in more than half of our cases of drug eruption.

Academic Significance and Societal Importance of the Research Achievements

再発予防の観点から、薬疹の原因薬剤の同定は非常に重要だが、既存の検査方法ではその陽性率が高くないことから、同定できないケースも多数存在する。既存の方法では同定できなくても、今回我々が開発した、皮膚浸潤T細胞を用いた手法により、同定できたケースも存在し、調べた過半数のケースで同定できたことから、有用な手法と考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2018 2016

All Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] Skin-Infiltrating MDR1-Expressing T Cells Are Associated With Therapeutic Responsiveness To Corticosteroids In Severe Cutaneous Adverse Reactions Of Drugs2018

    • Author(s)
      Toshiharu Fujiyama
    • Organizer
      Drug Hypersensitivity Meeting
    • Related Report
      2018 Annual Research Report
  • [Presentation] 皮膚湿潤T細胞を用いたELISpot法で原因薬剤同定を試みたSJS/TEN2016

    • Author(s)
      藤山俊晴
    • Organizer
      第46回日本皮膚アレルギー・接触皮膚炎学会総会学術大会.
    • Place of Presentation
      東京
    • Year and Date
      2016-11-05
    • Related Report
      2016 Research-status Report
  • [Presentation] Novel lymphocyte stimulation tests using ex-vivo expanded skin-infiltrating T cells from severe drug eruptions.2016

    • Author(s)
      藤山俊晴
    • Organizer
      46th Annual ESDR Meeting in 2016
    • Place of Presentation
      Munich
    • Year and Date
      2016-09-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] A novel method to determine causative drugs using ex-vivo expanded skin-infiltrating T cells from severe drug eruptions.2016

    • Author(s)
      藤山俊晴
    • Organizer
      第65回日本アレルギー学会学術大会.
    • Place of Presentation
      東京
    • Year and Date
      2016-06-18
    • Related Report
      2016 Research-status Report
  • [Presentation] Ex-Vivo Expanded Skin-Infiltrating T Cells From Severe Drug Eruptions Are Reactive With Causative Drugs: A Possible Novel Method For Determination Of Causative Drugs.2016

    • Author(s)
      藤山俊晴
    • Organizer
      7th Drug Hypersensitivity Meeting
    • Place of Presentation
      Malaga
    • Year and Date
      2016-04-20
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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