Development of new treatment targeting thyroid cancer stem cells
| Project/Area Number |
16K10467
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
Hayase Suguru 福島県立医科大学, 医学部, 学内講師 (00583387)
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| Co-Investigator(Kenkyū-buntansha) |
岩舘 学 福島県立医科大学, 医学部, 講師 (00381393)
鈴木 眞一 福島県立医科大学, 医学部, 教授 (70235951)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 甲状腺癌 / 幹細胞 / 分化誘導 / Nkx2-1 / 未分化癌 / Side population cell / 甲状腺 / 癌幹細胞 |
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| Outline of Final Research Achievements |
In this study, we established Side Population Cell-derived thyroid cell line (SPTL), cell line of mouse thyroid Side Population (SP) cells, to analyze the mechanism of differentiation induction of anaplastic thyroid cancer. By analyzing the gene expression profiles of this SPTL and anaplastic thyroid cancer, we found the group of genes whose expression is increased or decreased. We constructed a doxycycline-inducible Nkx2-1 expressing cell line, and confirmed that Nkx2-1 induced phosphorylation of AKT and that the pathway of AKT is essential in thyroid differentiation.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の学術的意義は甲状腺未分化癌を分化誘導することにより癌の浸潤および転移を制御することにある。さらに、甲状腺幹細胞との比較検討をすることにより甲状腺未分化癌は甲状腺幹細胞と似たような遺伝子発現プロファイルをもつことが確認された。甲状腺幹細胞と癌の分化のメカニズムを解析することにより、新たな癌治療の概念を生み出す大きな意義があると考える。
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Report
(5 results)
Research Products
(13 results)
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[Journal Article] Identification of Three Novel Fusion Oncogenes, SQSTM1/NTRK3, AFAP1L2/RET, and PPFIBP2/RET, in Thyroid Cancers of Young Patients in Fukushima.2017
Author(s)
Iyama K, Matsuse M, Mitsutake N, Rogounovitch T, Saenko V, Suzuki K, Ashizawa M, Ookouchi C, Suzuki S, Mizunuma H, Fukushima T, Suzuki S, Yamashita S.
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Journal Title
Thyroid
Volume: 印刷中
Issue: 6
Pages: 811-818
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] An Adult Mouse Thyroid Side Population Cell Line that Exhibits Enriched Epithelial-Mesenchymal Transition.2017
Author(s)
Murata T, Iwadate M, Takizawa Y, Miyakoshi M, Hayase S, Yang W, Cai Y, Yokoyama S, Nagashima K, Wakabayashi Y, Zhu J, Kimura S.
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Journal Title
Thyroid
Volume: 27
Issue: 3
Pages: 460-474
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The protocol and preliminary baseline survey results of the thyroid ultrasound examination in Fukushima [Rapid Communication]2016
Author(s)
Suzuki S, Yamashita S, Fukushima T, Nakano K, Midorikawa S, Ohtsuru A, Yasumura S, Hosoya M, Kamiya K, Shimura H, Suzuki S, Nakamura I, Abe M.
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Journal Title
Endocrine Journal
Volume: 63
Issue: 3
Pages: 315-321
DOI
NAID
ISSN
0918-8959, 1348-4540
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Inappropriate Suppression of Thyrotropin Cocentrations in Young Patients with Thyroid Nodules Including Throid Cancer: The Fukushima Health Management Survey.2016
Author(s)
Suzuki S, Nakamura I, Suzuki S, Ohkouchi C, Mizunuma H, Midorikawa S, Fukushima T, Ito Y, Shimura H, Ohira T, Matsuzaka T Ohtsuru A, Abe M Yamashita S, Suzuki S.
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Journal Title
Thyroid
Volume: 26(5)
Issue: 5
Pages: 717-725
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Comprehensive survey results of childhood thyroid ultrasound examinations in Fukushima in the first four years after the Fukushima Daiichi Nuclear Power Plant accident.2016
Author(s)
Suzuki S, Suzuki S, Fukushima T, Midorikawa S, Shimura H, Matsuzaka T, Ishikawa T, Takahashi H, Ohtsuru A, Sakai A, Hosoya M, Yasumura S, Nollet KE, Ohira T, Ohto H, Abe M, Kamiya K, Yamashita S.
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Journal Title
Thyroid.
Volume: 26(6)
Issue: 6
Pages: 843-851
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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