Evakuation of molecular target on circulationg tumor cells in gastrointestinal cancer patients

• Project/Area Number: 16K10509
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kagoshima University
• Principal Investigator: UENOSONO YOSHIKAZU 鹿児島大学, 医歯学総合研究科, 客員研究員 (60398279)
• Co-Investigator(Kenkyū-buntansha): 有上 貴明 鹿児島大学, 医歯学総合研究科, 特任准教授 (40527058) ; 夏越 祥次 鹿児島大学, 医歯学域医学系, 教授 (70237577)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: CTC / 消化器癌

Outline of Final Research Achievements

We compared Isoflux system with CellSearch system, but was not able to prove the usefulness of Isoflux system.Trastuzumab which was a molecular target drug was effective in the patient with HER2 expression of Circulating tumor cells was confirmed in gastric cancer patients. Furthermore, we evaluated the mRNA by the RT-PCR method with the blood of gastric cancer patients about PDL-1. Expression of PDL-1 was significantly high in the advanced cancer patients in comparison with early cancer patient. In addition, in the patients whom mRNA expression of PDL-1 was high in, the thing that had a poor prognosis was confirmed in intentionality in comparison with a low patient.

Academic Significance and Societal Importance of the Research Achievements

胃癌治療における血中の循環癌細胞の蛋白発現および血液中のmRNAを評価することにより、効率的な分子標的薬剤の適応を非侵襲的にCTCによる判別する方法を確立するために有益な知見が得られたものと考えられ、効果的な治療対象となる患者の選択が可能となるかもしれない。

Research Products

• [Journal Article] The association of human endogenous retrovirus-H long terminal repeat-associating protein 2 (HHLA2) expression with gastric cancer prognosis (2018)
  • Author(s): Shimonosono Masataka、Arigami Takaaki、Yanagita Shigehiro、Matsushita Daisuke、Uchikado Yasuto、Kijima Yuko、Kurahara Hiroshi、Kita Yoshiaki、Mori Shinichiro、Sasaki Ken、Omoto Itaru、Maemura Kosei、Uenosono Yoshikazu、Ishigami Sumiya、Natsugoe Shoji
  • Journal Title: Oncotarget Volume: 9 Issue: 31 Pages: 22069-22078
  • DOI: 10.18632/oncotarget.25179
  • Peer Reviewed / Open Access
• [Journal Article] Programmed death-ligand 1 is a promising blood marker for predicting tumor progression and prognosis in patients with gastric cancer. (2018)
  • Author(s): Amatatsu M, Arigami T, Uenosono Y, Yanagita S, Uchikado Y, Kijima Y, Kurahara H, Kita Y, Mori S, Sasaki K, Omoto I, Maemura K, Ishigami S, Natsugoe S.
  • Journal Title: Cancer Sci. Volume: 109 Issue: 3 Pages: 814-820
  • DOI: 10.1111/cas.13508
  • Peer Reviewed / Open Access
• [Journal Article] Clinical significance of altering epithelial-mesenchymal transition in metastatic lymph nodes of gastric cancer. (2017)
  • Author(s): Okubo K, Uenosono Y, Arigami T, Yanagita S, Matsushita D, Kijima T, Amatatsu M, Uchikado Y, Kijima Y, Maemura K, Natsugoe S.
  • Journal Title: Gastric Cancer Volume: － Issue: 5 Pages: 802-810
  • DOI: 10.1007/s10120-017-0705-x
  • NAID: 40021337917
  • Peer Reviewed
• [Presentation] 胃癌における末梢血および門脈血中の循環癌細胞の臨床的意義の検討 (2017)
  • Author(s): 1.上之園芳一, 有上貴明, 大久保啓史, 貴島孝, 柳田茂寛, 松下大輔, 小園勉, 天辰仁彦, 川越浩輔, 奥村浩, 石神純也, 帆北修一, 夏越祥次
  • Organizer: 第89回日本胃癌学会総会
• [Presentation] 切除不能・再発胃癌における化学療法によるHER2陽性の血中循環癌細胞の変化に関する検討 (2017)
  • Author(s): 3.上之園芳一, 有上貴明, 大久保啓史, 貴島孝, 柳田茂寛, 天辰仁彦, 川越浩輔,松下大輔, 奥村浩, 石神純也, 夏越祥次
  • Organizer: 第117回日本外科学会定期学術集会
• [Presentation] Utility of intraperitoneal paclitaxel chemotherapy for gastric cancer with peritoneal dissemination (2017)
  • Author(s): 7.Uenosono Y., Arigami T., Okubo K., Yanagita S., Kijima T., Amatatsu M, Matsushita D., Okumura H, Ishigami S, Natsugoe S.
  • Organizer: 47th World Congress of Surgery