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Prevention of rotator cuff tear via supression of glycation and oxidantative stress

Research Project

Project/Area Number 16K10859
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionKobe University

Principal Investigator

Mifune Yutaka  神戸大学, 医学部附属病院, 助教 (80608464)

Co-Investigator(Kenkyū-buntansha) 国分 毅  神戸大学, 医学研究科, 医学研究員 (40403266)
乾 淳幸  神戸大学, 医学部附属病院, 特命助教 (70457092)
Research Collaborator NISHIMOTO hanako  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords糖化最終生成物 / 活性酸素 / 肩腱板断裂 / 終末糖化産物 / アポシニン / 反復性低血糖モデル / 糖尿病モデル / 腱板変性断裂
Outline of Final Research Achievements

We successfully developed a mouse model with enhanced expression of AGEs/ROS. Then, in the in vitro study, it was confirmed that apocynin could decrease the expressions of NOX-1、NOX-4 and IL-6 in a high glucose condition, and we also proved that apocynin could inhibit an enhancement of ROS accumulation and apoptosis induced by high glucose. In the in vivo study, we injected collagenase in the Achilles tendon of DM rats to make an acute inflammation tendinitis model. Then, apocynin was injected to the rats in the treated group, resulting that the expressions of NOX、TNF-α、IL-6 were significantly decreased in the treated group when compared with the control group.

Academic Significance and Societal Importance of the Research Achievements

現在、肩腱板断裂における研究はバイオメカニカルな研究が主眼であることが多く、修復に難渋するような広範囲な断裂に対しては、人工材料の研究や幹細胞を用いた腱板再生医療など、バイオロジカルなアプローチが検討されている。しかし、これらは全て断裂した腱板をどのように修復するかという観点でしか検討されていない。今回の我々の研究は、肩腱板の断裂をいかにして予防するか、という予防医学的な観点での発想であり、これまでの研究とは全く異っており、今回の研究結果も踏まえて、将来的にAGEs/ROS阻害薬により加齢による肩腱板組織の変性断裂を予防できる新しい治療法の開発につながることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2019 2018

All Presentation (6 results) (of which Int'l Joint Research: 3 results)

  • [Presentation] Apocynin can exert inhibitory effects for AGEs-induced oxidative stress in rotator cuff derived cells2019

    • Author(s)
      Takashi Kurosawa, Yutaka Mifune
    • Organizer
      Orthopardic Reseach Society Annual Meeting 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of apocynin in vitro for high glucose-induced oxidative stress on tenocytes2018

    • Author(s)
      Takashi Kurosawa, Yutaka Mifune
    • Organizer
      Orthopardic Reseach Society Annual Meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高血糖ストレスモデルを用いたラット腱細胞におけるアポシニンの抗酸化作用の検討2018

    • Author(s)
      黒澤堯、美舩泰
    • Organizer
      第61回日本手外科学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アポシニンは肩腱板由来細胞において終末糖化産物による酸化ストレスに対して抑制効果を示す2018

    • Author(s)
      黒澤堯、美舩泰
    • Organizer
      第33回日本整形外科学会基礎学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Evaluation of apocynin in vitro for high glucose-induced oxidative stress on tenocytes2018

    • Author(s)
      黒澤 堯
    • Organizer
      Orthopaedic research society 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 高血糖ストレスモデルを用いたラット腱細胞におけるアポシニンの抗酸化作用の検討2018

    • Author(s)
      黒澤 堯
    • Organizer
      第61回日本手外科学会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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