Proteomic study using clinical materials for metastatic bone tumor toward the understanding of molecular backgrounds and the development of novel therapy
Project/Area Number |
16K10880
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | National Cancer Center Japan |
Principal Investigator |
Kondo Tadashi 国立研究開発法人国立がん研究センター, 研究所, 分野長 (30284061)
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Co-Investigator(Kenkyū-buntansha) |
穴澤 卯圭 東京歯科大学, 歯学部, 教授 (20245525)
菊田 一貴 東京歯科大学, 歯学部, 非常勤講師 (30383798)
多田 裕司 千葉大学, 大学院医学研究院, 特任教授 (50344990)
川井 章 国立研究開発法人国立がん研究センター, 中央病院, 科長 (90252965)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 転移性骨腫瘍 / バイオマーカー / プロテオーム解析 / プロテオーム / PDX |
Outline of Final Research Achievements |
This study was conducted to understanding of disease mechanisms of metastatic bone tumors, and to develop biomarkers. This study was characterized by the use of clinical specimens and proteomics data. Large-format two-dimensional difference gel electrophoresis and mass spectrometry were employed in this study. We created the protein expression profiles of metastatic tumors, identifying the proteins unique to tumors, and found biomarker candidates as proteins which could be released from the tumors and exist in the blood stream. Using the plasma samples, we validated the presence of identified proteins in the patients with or without bone metastasis. In addition, we investigated the protein expression differences among the metastatic bone tumors with different original sites. Those proteins could be candidates of biomarkers for differential diagnosis of malignancies of unknown origin.
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Academic Significance and Societal Importance of the Research Achievements |
骨は肺に次いで転移の多い臓器であり、多くの悪性腫瘍は骨に転移する。骨転移は予後不良因子であり、病的骨折を来すなどして患者のQOLを著しく損なう。しかしながら、転移性骨腫瘍の早期診断や有効な治療法は限られている。高齢化に伴うがん患者の増加および治療法の進歩により、骨に転移をもって長期生存する症例は増えており、転移性骨腫瘍の治療法の開発はますます重要になっている。その原発腫瘍の性状や分子背景により、転移性骨腫瘍は複雑な病態を示すが、適切な実験モデルが得難い。臨床検体を用いたオミクス解析のデータによって分子背景を網羅的に理解することで、有用なバイオマーカーなど新しい発見を期待することができる。
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] A serum microRNA classifier for the diagnosis of sarcomas of various histological subtypes.2019
Author(s)
Asano N, Matsuzaki J, Ichikawa M, Kawauchi J, Takizawa S, Aoki Y, Sakamoto H, Yoshida A, Kobayashi E, Tanzawa Y, Nakayama R, Morioka H, Matsumoto M, Nakamura M, Kondo T, Kato K, Tsuchiya N, Kawai A, Ochiya T
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Journal Title
Nature Communications
Volume: 10
Issue: 1
Pages: 1299-1299
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] HDAC2 regulates site-specific acetylation of MDM2 and its ubiquitination signaling in tumor suppression.2019
Author(s)
Patel N, Wang J, Shiozawa K, Jones KB, Zhang Y, Prokop JW, Davenport GG, Nihira NT, Hao Z, Wong D, Brandsmeier L, Meadows SK, Sampaio AV, Werff RV, Endo M, Capecchi MR, McNagny KM, Mak TW, Nielsen TO, Underhill TM, Myers RM, Kondo T, Su L.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Establishment and proteomic characterization of patient-derived clear cell sarcoma xenografts and cell lines2018
Author(s)
Sakumoto M, Oyama R, Takahashi M, Takai Y, Kito F, Shiozawa K, Qiao Z, Endo M, Yoshida A, Kawai A, Kondo T
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Journal Title
In Vitro Cell Dev Biol Anim
Volume: 54
Issue: 2
Pages: 163-176
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Establishment and characterization of cell line of undifferentiated pleomorphic sarcoma2017
Author(s)
Takai Y, Oyama R, Kito F, Sakumoto M, Shiozawa K, Qiao Z, Nakajima K, Takahashi M, Yoshida A, Setsu N, Kobayashi E, Kawai A, Kondo T
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Journal Title
Tiss Cult Res Commun
Volume: 36
Pages: 41-48
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] A novel p53 target gene FUCA1 encodes a fucosidase and regulates growth and survival of cancer cells.2016
Author(s)
Ezawa I, Sawai Y, Kawase T, Okabe A, Tsutsumi S, Ichikawa H, Kobayashi Y, Tashiro F, Namiki H, Kondo T, Semba K, Aburatani H, Taya Y, Nakagama H, Ohki R.
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Journal Title
Cancer Sci.
Volume: 印刷中
Issue: 6
Pages: 734-745
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Sacral Tuberculosis2016
Author(s)
0kuyama Y, Baba A, Kobashi Y, Anazawa U
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Journal Title
Internal Medicine
Volume: 55
Issue: 14
Pages: 1939-1940
DOI
NAID
ISSN
0918-2918, 1349-7235
Related Report
Peer Reviewed / Open Access
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[Journal Article] Diagnosis and treatment of bone metastasis: comprehensive guideline of the Japanese Society of Medical Oncology, Japanese Orthopedic Association, Japanese Urological Association, and Japanese Society for Radiation Oncology2016
Author(s)
Shibata H, Kato S, Sekine I, Abe K, Araki N, Iguchi H, Izumi T, Inaba Y, Osaka I, Kato S, Kawai A, Kinuya S, Kodaira M, Kobayashi E, Kobayashi T, Sato J, Shinohara N, Takahashi S, Takamatsu Y, Takayama K, Takayama K, Tateishi U, Nagakura H, Hosaka M, Morioka H, Moriya T, Yuasa T, Yurikusa T, Yomiya K, Yoshida M
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Journal Title
ESMO Open
Volume: 1
Issue: 2
Pages: e000037-e000037
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 肉腫のプロテオーム解析2016
Author(s)
近藤格
Organizer
第89回日本整形外科学会学術総会
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2016-05-12
Related Report
Invited
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