Establishment of rapid detection and epidemiological method of Multi-drug resistant bacteria in urinary tract infection
| Project/Area Number |
16K11047
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大澤 佳代 神戸大学, 保健学研究科, 准教授 (50324942)
荒川 創一 神戸大学, 医学研究科, 客員教授 (70159490)
白川 利朗 神戸大学, 科学技術イノベーション研究科, 教授 (70335446)
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| Research Collaborator |
Kitagawa Koichi
Yamamichi Fukashi
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 尿路感染症 / 薬剤耐性菌 / 迅速診断法 / 泌尿器科学 |
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| Outline of Final Research Achievements |
We studied MALDI-TOF-MS using the principle of Mass spectrometry for the purpose of rapid diagnosis of urinary tract infections, and demonstrated improved method for gram-positive bacteria where we had troubles for their detection, and presented the paper. Next, we performed the basic experiment Multiple-Locus Variable Number Tandem-Repeat Analysis: MLVA for rapid epidemiological diagnosis of Escherichia coli and showed the efficacy on that purpose. That is, using the carbapenem-resistant E. coli (n=23) and extended- spectrum beta- lactamase ESBL producing E. coli (n=67) and we extracted those bacteria with different repeat number in every region and decided repeat number. Then, it was diagnosed as closely related bacteria from tree diagram.
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| Academic Significance and Societal Importance of the Research Achievements |
近年の薬剤耐性細菌の増加に伴い、かつ国家レベルでの抗菌薬使用制限、適正使用指導の徹底が求められている。本MALDI-TOF MSの研究では上記の2方法にて尿路感染症の原因菌の迅速診断法の確立し、従来の尿培養検査よりは数日以上早く適切な治療を開始できることを提言できた。またMLVA法においては、特に疫学調査としての菌の伝播形式を迅速に視覚的に診断できる方法を尿路感染症の主要な原因菌である、それも薬剤耐性の大腸菌を用いて確立した。これにより特に院内感染の早期診断に役立ち、一刻も早い解決策の立案に貢献できる。
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Molecular characteristics of carbapenem-resistant Pseudomonas aeruginosa isolated from urine in Hyogo, Japan.2019
Author(s)
Osawa K, Shigemura K, Kitagawa K, Fukuda T, Takasaka A, Wakabayashi S, Sato K, Yamamichi F, Shirakawa T, Fujisawa M.
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Journal Title
Int J Urol.
Volume: 26
Issue: 1
Pages: 127-133
DOI
Related Report
Peer Reviewed
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[Journal Article] Acquisition of antimicrobial-resistant variants in repeated infections caused by Pseudomonas aeruginosa revealed by whole genome sequencing.2019
Author(s)
Nakanishi N, Nomoto R, Sato K, Koike C, Kusuki M, Nakamura T, Shigemura K, Shirakawa T, Fujisawa M, Tokimatsu I, Osawa K.
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Journal Title
J Infect Chemother.
Volume: 25
Issue: 2
Pages: 154-156
DOI
Related Report
Peer Reviewed
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[Journal Article] Occurrence and characterization of carbapenem-resistant Gram-negative bacilli: A collaborative study of antibiotic-resistant bacteria between Indonesia and Japan.2018
Author(s)
Kuntaman K, Shigemura K, Osawa K, Kitagawa K, Sato K, Yamada N, Nishimoto K, Yamamichi F, Rahardjo D, Hadi U, Mertaniasih NM, Kinoshita S, Fujisawa M, Shirakawa T.
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Journal Title
Int J Urol.
Volume: 25
Issue: 11
Pages: 966-972
DOI
Related Report
Peer Reviewed
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