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Retinal protection by changing gene expressions for glaucoma therapy

Research Project

Project/Area Number 16K11308
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

HARADA Chikako  公益財団法人東京都医学総合研究所, 運動・感覚システム研究分野, 研究員 (20435720)

Research Collaborator HARADA takayuki  
NAMEKATA kazuhiko  
KAKU gyorei  
KIMURA atsuko  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords緑内障 / 神経保護 / 軸索再生 / 遺伝子発現 / 視神経外傷 / 既存薬 / 神経栄養因子
Outline of Final Research Achievements

We demonstrated the neuroprotective effects of existing drugs, such as Rho/ROCK inhibitor eye drops, edaravone used for cerebral infarction, and N-acetylcysteine used as expectorant, and published these findings. In addition, we found that loss of neuritin, one of the trophic factors, increases neural cell death after optic nerve injury. We also found that loss of TrkB, one of the major neurotrophin receptors, induces normal tension glaucoma-like retinal degeneration after 6 months of age.

Academic Significance and Societal Importance of the Research Achievements

緑内障は我が国における最大の失明原因であり、眼圧降下以外の手法による神経保護療法の開発が期待されている。そこで本研究では複数の緑内障モデル動物を用いて、安全性が確立した既存薬による細胞保護療法の可能性を示した。また特定の遺伝子発現の変化が神経細胞の耐性に与える影響を検討して、将来の薬物開発や新たな疾患モデルの開発に道をつけた。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (35 results)

All 2019 2018 2017 2016 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 9 results,  Acknowledgement Compliant: 5 results) Presentation (17 results) (of which Int'l Joint Research: 11 results) Remarks (6 results)

  • [Journal Article] Differential effects of N-acetylcysteine on retinal degeneration in two mouse models of normal tension glaucoma2019

    • Author(s)
      Sano Hiroki、Namekata Kazuhiko、Kimura Atsuko、Shitara Hiroshi、Guo Xiaoli、Harada Chikako、Mitamura Yoshinori、Harada Takayuki
    • Journal Title

      Cell Death & Disease

      Volume: 10 Issue: 2 Pages: 75-75

    • DOI

      10.1038/s41419-019-1365-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent advances in genetically modified animal models of glaucoma and their roles in drug repositioning2018

    • Author(s)
      Harada Chikako、Kimura Atsuko、Guo Xiaoli、Namekata Kazuhiko、Harada Takayuki
    • Journal Title

      British Journal of Ophthalmology

      Volume: 103 Issue: 2 Pages: 161-166

    • DOI

      10.1136/bjophthalmol-2018-312724

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma2018

    • Author(s)
      Akaiwa Kei、Namekata Kazuhiko、Azuchi Yuriko、Sano Hiroki、Guo Xiaoli、Kimura Atsuko、Harada Chikako、Mitamura Yoshinori、Harada Takayuki
    • Journal Title

      Investigative Opthalmology & Visual Science

      Volume: 59 Issue: 5 Pages: 2080-2089

    • DOI

      10.1167/iovs.17-23276

    • NAID

      120006956850

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Edaravone prevents retinal degeneration in adult mice following optic nerve injury.2017

    • Author(s)
      Akiyama G, Azuchi Y, Guo X, Noro T, Kimura A, Harada C, *Namekata K, Harada T
    • Journal Title

      Investigative Ophthalmology & Visual Science

      Volume: 58 Issue: 11 Pages: 4908-4914

    • DOI

      10.1167/iovs.17-22250

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] The renin-angiotensin system regulates neurodegeneration in a mouse model of optic neuritis2017

    • Author(s)
      Guo X., Namekata K., Kimura A., Harada C. & Harada T
    • Journal Title

      American journal of pathology

      Volume: 187 Issue: 12 Pages: 2876-2885

    • DOI

      10.1016/j.ajpath.2017.08.012

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Edaravone suppresses retinal ganglion cell death in a mouse model of normal tension glaucoma2017

    • Author(s)
      Akaiwa K., Namekata K., Azuchi Y., Guo X., Kimura A., Harada C., Mitamura Y. & Harada T
    • Journal Title

      Cell Death and Disease

      Volume: 8 Issue: 7 Pages: e2934-e2934

    • DOI

      10.1038/cddis.2017.341

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ASK1 in neurodegeneration2017

    • Author(s)
      Guo X., Namekata K., Kimura A., Harada C. & Harada T
    • Journal Title

      Advances in Biological Regulation

      Volume: 66 Pages: 63-71

    • DOI

      10.1016/j.jbior.2017.08.003

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Valproic acid and ASK1 deficiency ameliorate optic neuritis and neurodegeneration in an animal model of multiple sclerosis.2017

    • Author(s)
      Azuchi, Y., Kimura, A., Guo, X., Akiyama, G., Noro, T., Harada, C., Nishigaki, A., Namekata, K., Harada, T.
    • Journal Title

      Neuroscience Letters

      Volume: 639 Pages: 82-87

    • DOI

      10.1016/j.neulet.2016.12.057

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Targeting oxidative stress for treatment of glaucoma and optic neuritis.2017

    • Author(s)
      Kimura, A., Namekata, K., Guo, X., Noro, T., Harada, C., Harada, T.
    • Journal Title

      Oxidative Medicine and Cellular Longevity

      Volume: 2017 Issue: 1 Pages: 2817252-2817252

    • DOI

      10.1155/2017/2817252

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Dock3-NMDA receptor interaction as a target for glaucoma therapy.2017

    • Author(s)
      Kimura, A., Namekata, K., Guo, X., Harada, C., Harada, T.
    • Journal Title

      Histology and Histopathology

      Volume: 32 Pages: 215-221

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Neuroprotection, growth factors and BDNF-TrkB signaling in retinal degeneration.2016

    • Author(s)
      Kimura, A., Namekata, K., Guo, X., Harada, C., Harada, T.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 17 Issue: 9 Pages: 1584-1584

    • DOI

      10.3390/ijms17091584

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Caloric restriction promotes cell survival in a mouse model of normal tension glaucoma.2016

    • Author(s)
      Guo, X., Kimura, A., Azuchi, Y., Akiyama, G., Noro, T., Harada, C., Namekata, K., Harada, T.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 33950-33950

    • DOI

      10.1038/srep33950

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Dock10 deficiency reduces neuroinflammation in optic neuritis.2018

    • Author(s)
      Xiaoli Guo, Kazuhiko Namekata, Yuriko Azuchi, Atsuko Kimura, Takahiko Noro, Chikako Harada, Takayuki Harada.
    • Organizer
      ARVO Annual Meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Topical ripasudil prevents retinal ganglion cell death in a mouse model of normal tension glaucoma.2018

    • Author(s)
      Takayuki Harada, Kei Akaiwa, Kazuhiko Namekata, Yuriko Azuchi, Xiaoli Guo, Atsuko Kimura, Chikako Harada, Yoshinori Mitamura.
    • Organizer
      13th European Glaucoma Society Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Manipulation of retinal glutamate levels by overexpression of GLAST reduces retinal ganglion cell death in an experimental model of glaucoma.2018

    • Author(s)
      Atsuko Kimura, Kazuhiko Namekata, Xiaoli Guo, Yuriko Azuchi, Chikako Harada, Takayuki Harada.
    • Organizer
      19th International Congress of Neuropathology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DOCK8 regulates microglial activity in neuroinflammation.2018

    • Author(s)
      Kazuhiko Namekata, Xiaoli Guo, Atsuko Kimura, Nobutaka Arai, Chikako Harada, Takayuki Harada.
    • Organizer
      19th International Congress of Neuropathology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Can valproic acid treat neurodegenerative diseases?2018

    • Author(s)
      Atsuko Kimura, Kazuhiko Namekata, Xiaoli Guo, Yuriko Azuchi, Chikako Harada, Takayuki Harada.
    • Organizer
      International Conference on Neurovascular and Neurodegenerative Diseases
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Loss of neuritin accelerates RGC loss and retinal degeneration in adult mice following optic nerve injury.2018

    • Author(s)
      Yuriko Azuchi, Kazuhiko Namekata, Tadayuki Shimada, Xiaoli Guo, Atsuko Kimura, Chikako Harada, Atsuko Nishigaki, Kanato Yamagata, Takayuki Harada.
    • Organizer
      48th Annual Meeting of the Society for Neuroscience
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spontaneous normal tension glaucoma in aged marmosets.2018

    • Author(s)
      Takayuki Harada, Takahiko Noro, Kazuhiko Namekata, Atsuko Kimura, Nanako Hashimoto, Keiko Moriya-Ito, Xiaoli Guo, Chikako Harada, Tadashi Nakano, Takashi Inoue, Erika Sasaki.
    • Organizer
      8th World Glaucoma Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 正常眼圧緑内障モデルマウスを用いたEdaravoneによる網膜神経節細胞保護効果の検討2018

    • Author(s)
      赤岩慶、行方和彦、安土ゆり子、郭 暁麗、木村敦子、原田知加子、三田村佳典、原田高幸.
    • Organizer
      第122回 日本眼科学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カロリー制限による正常眼圧緑内障モデルにおける網膜神経節細胞及び視機能の保護2017

    • Author(s)
      郭 暁麗、木村 敦子、安土 ゆり子、秋山 悟一、野呂 隆彦、原田 知加子、行方 和彦、原田 高幸.
    • Organizer
      第121回 日本眼科学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] 細胞種特異的なASK1遺伝子の欠損が神経炎症に与える影響の検討2017

    • Author(s)
      郭 暁麗、行方和彦、木村敦子、原田知加子、新井信隆、原田高幸.
    • Organizer
      第58回 日本神経病理学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] 神経炎症におけるDock8の機能解析2017

    • Author(s)
      行方和彦、郭 暁麗、木村敦子、原田知加子、新井信隆、原田高幸.
    • Organizer
      第58回 日本神経病理学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Dock8 deficiency suppresses microglial migration and neuroinflammation.2016

    • Author(s)
      Kazuhiko Namekata, Xiaoli Guo, Atsuko Kimura, Chikako Harada, Takayuki Harada.
    • Organizer
      46th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Dietary restriction promotes cell survival in a mouse model of normal tension glaucoma.2016

    • Author(s)
      Xiaoli Guo, Kazuhiko Namekata, Atsuko Kimura, Takahiko Noro, Goichi Akiyama, Yuriko Azuchi, Chikako Harada, Takayuki Harada.
    • Organizer
      46th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Neuroprotection and optic nerve regeneration for glaucoma therapy: contribution of oxidative stress in animal models of normal tension glaucoma2016

    • Author(s)
      Takayuki Harada, Kazuhiko Namekata, Atsuko Kimura, Takahiko Noro, Chikako Harada.
    • Organizer
      XXII International Congress of Eye Research (ISER 2016)
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Overexpression of GLAST protects retinal ganglion cells following optic nerve injury.2016

    • Author(s)
      Atsuko Kimura, Kazuhiko Namekata, Xiaoli Guo, Yuriko Azuchi, Takahiko Noro, Goichi Akiyama, Chikako Harada, Takayuki Harada.
    • Organizer
      XXII International Congress of Eye Research (ISER 2016)
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ASK1 deficiency and valproic acid synergistically reduce demyelination and visual impairment in experimental autoimmune encephalomyelitis.2016

    • Author(s)
      Yuriko Azuchi, Atsuko Kimura, Xiaoli Guo, Takahiko Noro, Goichi Akiyama, Magdalena Droztz, Chikako Harada, Atsuko Saito, Kazuhiko Namekata, Takayuki Harada.
    • Organizer
      The 39th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] レニンーアンジオテンシン系は正常眼圧緑内障モデルマウスにおける神経 変性を制御する2016

    • Author(s)
      仙波賢太郎、行方和彦、郭 暁麗、原田知加子、原田高幸.
    • Organizer
      第120回 日本眼科学会総会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-04-07
    • Related Report
      2016 Research-status Report
  • [Remarks] 未来を話そう! プロジェクト研究の紹介

    • URL

      http://www.igakuken.or.jp/project/to-tomin/to-pro26.html

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Remarks] プロジェクト研究等 医学研で行われている研究活動

    • URL

      http://www.igakuken.or.jp/project/detail/retina.html

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Remarks] 網膜・視神経変性疾患の病態解明と治療法

    • URL

      http://www.igakuken.or.jp/retina/index.html

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Remarks]

    • URL

      http://www.igakuken.or.jp/retina/index.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 視覚病態プロジェクト

    • URL

      http://www.igakuken.or.jp/project/detail/retina.html

    • Related Report
      2016 Research-status Report
  • [Remarks] Visual Research Porject

    • URL

      http://www.igakuken.or.jp/english/project/detail/retina.html

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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