| Project/Area Number |
16K11428
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
九田 裕一 金沢医科大学, 医学部, 助教 (50566916)
谷田 守 金沢医科大学, 医学部, 准教授 (70512309)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Angiopoietin 2 / アナフィラキシーショック / マウス / ラット / 血管透過性亢進 / ヘマトクリット / angiopoietin 2 / 血管透過性 / anaphylactic shock / angipoietin-2 / vascular permeability / edema / Anaphylactic shock / Mouse / Rat / Vascular permeability / 動物実験 / 生理学 / アレルギー / アナフィラキシー |
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| Outline of Final Research Achievements |
Angiopoietin (Angpt)-2, an endothelium derived permeability-increasing factor, is involved in vascular leakage of sepsis, but its role remains unknown in anaphylactic hyperpermeability. We determined plasma Angpt-2 levels and hematocrit (Hct) as a measure of vascular leak during anaphylactic hypotension in ovalbumin-sensitized mice and rats. In both animals, following antigen injection, vascular permeability increased at 20 min, and Hct increased reaching the peak within 20 min, followed by a progressive decrease in rats. The plasma Angpt-2 levels did not change significantly within 20 min and until 60 min in mice and rats, respectively. Thereafter, in awake rats, they reached the peak at 6 h after antigen, and returned to the baseline at 24 h. In addition, in mice, western blot analysis revealed no increase in tissue Angpt-2 within 20 min after antigen. In conclusion, Angpt-2 is not released at early phase when vascular leak occurs during anaphylactic hypotension in mice and rats.
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| Academic Significance and Societal Importance of the Research Achievements |
アナフィラキシーショックは致死的となりうる救急疾患で、更なる病態解明が求められている。その病態の特徴として血管透過性亢進がある。本研究の目的は敗血症などの血管透過性亢進に関わるAngiopoietin(Angpt)-2がアナフィラキシーへの関与を動物実験で解明することであった。その結果、ラットとマウスともにアナフィラキシー低血圧時に血管透過性は抗原投与20分以内の早期に亢進したが、その時にはAngpt-2の血中と組織中の濃度増加はなく、血管透過性亢進への関与は低いことが明らかとなった。本研究成果は致死的病態であるアナフィラキシー低血圧時の血管透過性亢進の病態の解明に貢献するものと考えられる。
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