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Prevention of surgical ileus with intraperitoneal administration of hydrogen-rich saline

Research Project

Project/Area Number 16K11430
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Emergency medicine
Research InstitutionOkayama University

Principal Investigator

NAKAO ATSUNORI  岡山大学, 医歯薬学総合研究科, 教授 (40648169)

Co-Investigator(Kenkyū-buntansha) 石川 倫子  兵庫医科大学, 医学部, 非常勤講師 (40566121)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords水素 / イレウス / 腹腔内投与 / 腸管 / 炎症 / 水素ガス / 水素豊富食塩水 / 水素豊富生理食塩水 / 術後腸管麻痺 / 腸管輸送能 / 炎症細胞 / 外科
Outline of Final Research Achievements

Ileus was induced via surgical manipulation (SM) of male C57BL/6 mice. After SM, the peritoneal cavity was filled with 1.0 mL of either physiological saline or hydrogen-saturated physiological saline. SM resulted in a marked delay in intestinal transit associated with proinflammatory cytokine expression compared with sham controls. Bowel dysmotility induced by SM was attenuated by intraabdominal administration of hydrogen-saturated saline. Hydrogen-saline significantly reduced the upregulation of mRNAs of inflammatory markers. SM was associated with extensive leukocyte infiltration 24 hrs after SM when treated with saline; however, this extravasation was significantly decreased when hydrogen-saline was i.p. administered. While NO production increased after SM, hydrogen significantly reduced NO levels in organ culture six hours after SM.
A single i.p. dose of hydrogen-saturated physiological saline ameliorates postoperative ileus by inhibiting the inflammatory response and NO production.

Academic Significance and Societal Importance of the Research Achievements

術後イレウスは重要な術後合併症であり、入院期間の延長と医療費の増加につながり、臨床上の大きな問題である。術後イレウスの患者は、経管栄養がすすまず、また嘔吐に続発する誤嚥性肺炎の合併など治療に難渋することをしばしば経験し、現行の治療の限界を感じてきた。水素を水溶液として用いることで、水素の起爆性の問題がクリアでき医療の現場では受け入れられやすい。また、開腹手術では生理食塩水で腹腔内を洗浄する操作も日常的に行われるため、開腹手術のストラテジーを大きく変える必要がなく、臨床応用が容易である。本方法の有効性が実証されれば、医療経済的観点からも意義が大きいと思われる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] inhaled hydrogen ameliorates endotoxin-induced bowel dysfunction2017

    • Author(s)
      Hiroyuki Sakata, Ayana Okamoto, Michiko Aoyama-Ishikawa,Hayato Yamashita, Keisuke Kohama, Noritomo Fujisaki,Taihei Yamada,Joji Kotani, Kohei Tsukahara, Atsuyoshi Iida and Atsunori Nakao
    • Journal Title

      Acute Medicine & Surgery

      Volume: 4 Issue: 1 Pages: 38-45

    • DOI

      10.1002/ams2.218

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intraperitoneally administered, hydrogen-rich physiologic solution protects against postoperative ileus and is associated with reduced nitric oxide production2016

    • Author(s)
      Okamoto A, et al.
    • Journal Title

      Surgery

      Volume: 160 Issue: 3 Pages: 623-631

    • DOI

      10.1016/j.surg.2016.05.026

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Pneumoperitoneum with hydrogen-containing carbon dioxide ameliorates bowel inflammation2018

    • Author(s)
      Yamamoto H, Yamada T, Yumoto T, Osako T, Naito H, Nakao A
    • Organizer
      International Conference on Emergency Medicine
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pneumoperitoneum with hydrogen-contained carbon dioxidce ameliorates bowel inflammation2017

    • Author(s)
      Taihei Yamada, Tetsuya Yumoto, Takaaki Osako, Hiromichi Naito, Atsunori Nakao
    • Organizer
      Asian Conference on Emergency Medicine
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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