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Complete analysis of the composition and biosynthesis mechanism of specific O-polysaccharides present in a periodontal pathogen's LPS

Research Project

Project/Area Number 16K11451
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionNagasaki University

Principal Investigator

SHOJI Mikio  長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10336175)

Co-Investigator(Kenkyū-buntansha) 橋本 雅仁  鹿児島大学, 理工学域工学系, 教授 (30333537)
Research Collaborator NAKAYAMA Koji  
YUKITAKE Hideharu  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords歯周病関連細菌 / リポ多糖 / 歯周病細菌
Outline of Final Research Achievements

Porphyromonas gingivalis is known as a keystone pathogen of chronic periodontitis. This bacterium secretes many virulence proteins via the type 9 secretion mechanism (T9SS). Some of the T9SS cargo proteins are covalently bound to A-LPS and are anchored on the cell surface. This bacterium has two kinds of LPSs, A-LPS and O-LPS, however, their biosynthesis mechanism is unknown. In this study, we analyzed the biosynthesis mechanism of O polysaccharide of both LPSs.
About 14 glycosyltransferases that have not been characterized from genomic information, we tried to create their gene mutants. From the mutant study analysis, some genes involved in O-polysaccharide biosynthesis in A-LPS and O-LPS were identified, and the order of their biosynthesis was clarified.

Academic Significance and Societal Importance of the Research Achievements

我々は、歯周病細菌Porphyromonas gingivalisの病原性プロテアーゼなどの分泌タンパク質が新規の分泌装置である9型分泌機構によって菌体表面に輸送されること、さらに、菌体表面に出たこれらのタンパク質は本菌特異的な病原性LPSのO多糖鎖に結合することで局在化すること、を見出していた。この病原性LPSのO多糖鎖については我々の知見と相反する報告があり、問題となっていた。本研究はその問題に取り組み、O多糖鎖の生合成機構を新規に提唱した。本研究成果は、将来の応用研究に展開させるうえで重要な基盤となる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (25 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] The Scripps Research Institute/SLAC National Accelerator Laboratory(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] PGN_0297 is an essential component of the type IX secretion system (T9SS) in Porphyromonas gingivalis: Tn-seq analysis for exhaustive identification of T9SS-related genes.2019

    • Author(s)
      Naito M, Tominaga T, Shoji M, Nakayama K.
    • Journal Title

      Microbiol Immunol.

      Volume: 63(1) Issue: 1 Pages: 11-20

    • DOI

      10.1111/1348-0421.12665

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Immunoglobulin-like domains of the cargo proteins are essential for protein stability during secretion by the type IX secretion system.2018

    • Author(s)
      Sato K, Kakuda S, Yukitake H, Kondo Y, Shoji M, Takebe K, Narita Y, Naito M, Nakane D, Abiko Y, Hiratsuka K, Suzuki M, Nakayama K.
    • Journal Title

      Mol Microbiol.

      Volume: 110(1) Issue: 1 Pages: 64-81

    • DOI

      10.1111/mmi.14083

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis triggers NLRP3-mediated inflammasome activation of macrophages in a bacterial gingipains-independent manner.2018

    • Author(s)
      Tokuju Okano, Hiroshi Ashida, Shiho Suzuki, Mikio Shoji, Koji Nakayama, Toshihiko Suzuki,
    • Journal Title

      Eur. J. Immunol.

      Volume: 48 Issue: 12 Pages: 1965-1974

    • DOI

      10.1002/eji.201847658

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of genes encoding glycosyltransferases involved in lipopolysaccharide synthesis in Porphyromonas gingivalis.2018

    • Author(s)
      Shoji M, Sato K, Yukitake H, Kamaguchi A, Sasaki Y, Naito M, Nakayama K.
    • Journal Title

      Mol Oral Microbiol.

      Volume: 33(1) Issue: 1 Pages: 68-80

    • DOI

      10.1111/omi.12200

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 歯周病制圧に向けた最新の歯周病細菌の病原機構について2017

    • Author(s)
      庄子幹郎
    • Journal Title

      月刊細胞

      Volume: 49 (11) Pages: 538-541

    • Related Report
      2017 Research-status Report
  • [Journal Article] A Distinct Type of Pilus from the Human Microbiome.2016

    • Author(s)
      Xu Q, Shoji M, Shibata S, Naito M, Sato K, Elsliger MA, Grant JC, Axelrod HL, Chiu HJ, Farr CL, Jaroszewski L, Knuth MW, Deacon AM, Godzik A, Lesley SA, Curtis MA, Nakayama K, Wilson IA
    • Journal Title

      Cell

      Volume: 165 Issue: 3 Pages: 690-703

    • DOI

      10.1016/j.cell.2016.03.016

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Glycobiology of the oral pathogen Porphyromonas gingivalis and related species.2016

    • Author(s)
      Shoji M, Nakayama K
    • Journal Title

      Microb Pathog

      Volume: 94 Pages: 35-41

    • DOI

      10.1016/j.micpath.2015.09.012

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bacteroidia綱細菌群に存在するV型線毛の形成機構2016

    • Author(s)
      庄子幹郎, 中山浩次.
    • Journal Title

      臨床免疫・アレルギー科

      Volume: 66(6) Pages: 641-646

    • Related Report
      2016 Research-status Report
  • [Presentation] Porphyromonas gingivalis gingipains による炎症抑制機構2018

    • Author(s)
      岡野徳壽,庄子幹郎,中山浩次,鈴木敏彦
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Glycosyltransferase-encoding genes involved in LPS synthesis in Porphyromonas gingivalis2018

    • Author(s)
      庄子幹郎, 佐藤啓子, 雪竹英治, 内藤真理子, 中山浩次
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] A T9SS cargo protein involved in regulation of the T9SS expression in Porphyromonas gingivalis2018

    • Author(s)
      雪竹英治, 反田祐介, 門脇知子, 佐藤啓子, 庄子幹郎, 内藤真理子, 今田勝巳, 中山浩次
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] V型線毛のクライオ電子顕微鏡を用いた解析2018

    • Author(s)
      柴田敏史, 庄子幹郎, 中山浩次
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] The type V pili in the Bacteroidia class bacteria: novel assembly mechanism2017

    • Author(s)
      庄子幹郎, Xu Q, 柴田敏史, 内藤真理子, 佐藤啓子, Wilson IA, 中山浩次.
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター (宮城県・仙台市)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Porphyromonas ginigvalisのV型線毛の試験管内再構成による構造解析2017

    • Author(s)
      柴田敏史, 庄子幹郎, 中山浩次.
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター (宮城県・仙台市)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Porphyromonas gingivalisのIX型分泌機構の調節メカニズムに関与する分子の解析2017

    • Author(s)
      雪竹英治,門脇知子,内藤真理子,佐藤啓子, 庄子幹郎,菊池有一郎,中山浩次.
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター (宮城県・仙台市)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 高密度Tn-seq法によるIX型分泌機構関連遺伝子の探索2017

    • Author(s)
      内藤真理子,庄子幹郎,中山浩次
    • Organizer
      第70回日本細菌学会九州支部総会・第54回日本ウイルス学会九州支部総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Porphyromonas gingivalis LPSの生合成に関わる4つの糖転移酵素遺伝子の発見2017

    • Author(s)
      庄子幹郎, 佐藤啓子, 雪竹英治, 内藤真理子, 中山浩次
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Bacteroidia綱細菌に存在する線毛の新たな形成機構2016

    • Author(s)
      庄子幹郎, Xu Q, 柴田敏史, 内藤真理子,佐藤啓子, Wilson IA, 中山浩次.
    • Organizer
      第69回日本細菌学会九州支部総会・第53回日本ウイルス学会九州支部総会
    • Place of Presentation
      宮崎市民プラザ (宮崎県・宮崎市)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] Structural and mechanistic into a distinct type of pilus2016

    • Author(s)
      庄子幹郎, Xu Q, 柴田敏史, 内藤真理子, 佐藤啓子, Wilson IA,中山浩次.
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター (北海道・札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Biogenesis mechanism of the Type V pili present in the Bacteroidia class2016

    • Author(s)
      庄子幹郎, Xu Q, 柴田敏史, 内藤真理子, 佐藤啓子, Wilson IA, 中山浩次
    • Organizer
      新学術領域研究「運動超分子が織りなす調和と多様性」第4回領域全体会議
    • Place of Presentation
      長崎大学 (長崎県・長崎市)
    • Year and Date
      2016-06-08
    • Related Report
      2016 Research-status Report
  • [Remarks] 腸内の優勢細菌群の線毛形成機構の解明

    • URL

      http://www.nagasaki-u.ac.jp/ja/about/info/science/science104.html

    • Related Report
      2016 Research-status Report
  • [Remarks] ヒトの腸において優勢なBacteroidetes門細菌に見い出された線毛の新しい形成機構

    • URL

      http://first.lifesciencedb.jp/archives/12343

    • Related Report
      2016 Research-status Report
  • [Remarks] 長崎大学歯学部 歯周病基盤研究センター

    • URL

      http://www.de.nagasaki-u.ac.jp/perio/

    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] メンブレンヴェシクル2019

    • Inventor(s)
      中尾龍馬, 庄子幹郎, 中山浩次
    • Industrial Property Rights Holder
      中尾龍馬, 庄子幹郎, 中山浩次
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-02-16  

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