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Application of novel DDS strategies for induction and regeneration of periodontal tissues

Research Project

Project/Area Number 16K11458
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionJuntendo University

Principal Investigator

Takano Yoshiro  順天堂大学, 医学部, 非常勤講師 (90126425)

Co-Investigator(Kenkyū-buntansha) 大島 勇人  新潟大学, 医歯学系, 教授 (70251824)
田畑 純  東京医科歯科大学, 大学院医歯学総合研究科, 准教授 (20243248)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords歯周組織再生誘導 / ドラッグデリバリーシステム / 歯髄 / 象牙質 / 蛍光トレーサー / FGF2 / FGFR2 / 動物実験 / マウス / ドラッグデリバリー / 歯周組織 / 再生医療 / 動物実験モデル / 歯学 / 再生医学 / 細胞・組織
Outline of Final Research Achievements

To establish an efficient drug delivery system (DDS) applicable for periodontal tissue regeneration, we looked up a tooth remaining in the areas affected by periodontal diseases as a bio-ceramic drug delivery capsule, which should allow consistent delivery of bio-active agents from the pulp chamber to periodontal tissues via dentinal tubules. To explore applicability of novel DDS concept in dental clinics, we constructed an animal experimental model in which the upper molars were utilized as the drug delivery capsules.
After root canal treatment, emptied pulp chamber of the 1st molar or rat or mouse was filled with FGF2 or with normal saline. One to 4 weeks later, histology of periodontal tissues of the respective teeth were examined by various histological methods. The proposed animal experiment model of novel DDS was practical and easy to construct, but shown to require high skills successfully perform canal treatment in the narrow oral cavities of small animals.

Academic Significance and Societal Importance of the Research Achievements

歯周組織再生療法の期待が高まる中生理活性物質を局所に無駄なく長期間維持できる徐放性ドラッグデリバリーシステム(DDS)の開発が望まれている。歯の象牙質は無数の象牙細管で穿たれた多孔性の物質で、歯髄と外界を繋ぐ歯の物質拡散路として機能している可能性が高い。
我々は歯周病の患部に残存する「歯」を薬物チェンバー(歯髄腔)を内包する徐放性カプセルと位置づけ、残存歯の髄腔に再生誘導因子を封入して直近の患部組織へ徐放させる新規のDDSの開発とその応用を目指している。本研究ではラット/マウスの臼歯を徐放カプセルに見立てた動物実験系を構築し、臨床応用に向けて必要となるノウハウと基礎データを得ることができた。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (1 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Stim1 Regulates Enamel Mineralization and Ameloblast Modulation2017

    • Author(s)
      Y. Furukawa, N. Haruyama, M. Nikaido, M. Nakanishi, N. Ryu, M. Oh-Hora, K. Kuremoto, K. Yoshizaki, Y. Takano, I. Takahashi
    • Journal Title

      Journal of Dental Research

      Volume: 96 Issue: 12 Pages: 1422-1429

    • DOI

      10.1177/0022034517719872

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The sp7 gene is required for maturation of osteoblast-lineage cells in medaka (Oryzias latipes) vertebral column development2017

    • Author(s)
      Azetsu Y, Inohaya K, Takano Y, Kinoshita M, Tasaki M, Kudo A.
    • Journal Title

      Dev Biol

      Volume: 431(2) Issue: 2 Pages: 252-262

    • DOI

      10.1016/j.ydbio.2017.09.010

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The positional and ultrastructural changes in peripheral pulp capillaries correlate with the active phase of dentin deposit and mineralization in rat molars2017

    • Author(s)
      Yuta Seino, Yoshiro Takano, Hayato Ohshima
    • Journal Title

      Journal of Oral Biosciences

      Volume: 59(3) Issue: 3 Pages: 163-171

    • DOI

      10.1016/j.job.2017.05.006

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Structure and function of maturation ameloblasts - An oveview -2016

    • Author(s)
      Yoshiro Takano
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
    • Invited
  • [Book] 10万種類のタンパク質(ニュートン別冊)2016

    • Author(s)
      高野吉郎
    • Total Pages
      192
    • Publisher
      ニュートン・プレス
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

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