Detection and application of morphologically disease-specific markers in autoimmune salivary gland inflammation

• Project/Area Number: 16K11463
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tsurumi University
• Principal Investigator: Ito Yumi 鶴見大学, 歯学部, 講師 (00176372)
• Co-Investigator(Kenkyū-buntansha): 梁 洪淵 鶴見大学, 歯学部, 講師 (10298268) ; 美島 健二 昭和大学, 歯学部, 教授 (50275343) ; 斎藤 一郎 鶴見大学, 歯学部, 教授 (60147634)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: シェーグレン症候群 / 診断基準 / 腺組織障害 / 唾液分泌量 / 血清検査 / 組織検査 / 口唇腺生検 / 脂肪変性 / 自己免疫疾患 / 組織障害 / focus score / 自己抗体 / アポトーシス / 病理学 / 細胞・組織、 / 歯学

Outline of Final Research Achievements

Autoimmune sialadenitis (Sjögren's syndrome:SS) is an incurable disease, and there are a considerable number of patients including potential ones. However, we haven’t obtained unified diagnostic criteria and treatment policy. In this study, we focused on the importance of clarifying the pathogenesis of SS, and in order to understand the stage and causes of SS, we reevaluated all of 343 cases which a biopsy of the labial salivary gland had been performed. Then we digitized the degree of the lesion of labial salivary gland tissue, using clinical data including autoantibodies and saliva flow. The labial salivary gland biopsies which are used in all diagnostic criteria are highly estimated for their usefulness in understanding pathological conditions and stage of disease. SS is a disease for which understanding the pathogenesis is important. Therefore, we should consider that we’ll find less-invasive diagnostic method.

Academic Significance and Societal Importance of the Research Achievements

SSの診断基準の改定が進められる中，多数の生検材料と臨床データを用いた病態解析に基づく診断基準の検討が急務となっている．研究代表者らの施設ではドライマウス外来患者数が6,000人にのぼり，300人を超えるSS患者を経験し，国内の歯科大学附属病院の中では極めて豊富な臨床データを有している．この環境はSSに関する学術的な臨床研究を行うのに適していると言える．また歯科大学という特色からドライマウス外来担当の専門医と口腔病理医という専門性の高い視点から診断を行っているため臨床データの正確性は高い．この豊富な臨床データの解析は，病態解明や診断基準の策定に大いに貢献できる極めて社会的意義の高い研究である．

Research Products

• [Journal Article] Clinical practice guideline for Sj?gren’s syndrome 2017 (2018)
  • Author(s): Sumida Takayuki、Azuma Naoto、Moriyama Masafumi、Takahashi Hiroyuki、Asashima Hiromitsu、Honda Fumika、Abe Saori、Ono Yuko、Hirota Tomoya、Hirata Shintaro、Tanaka Yoshiya、Shimizu Toshimasa、Nakamura Hideki、Kawakami Atsushi、Sano Hajime、Ogawa Yoko、Tsubota Kazuo、Ryo Koufuchi、Saito Ichiro、Tsuboi Hiroto
  • Journal Title: Modern Rheumatology Volume: 28 Issue: 3 Pages: 383-408
  • DOI: 10.1080/14397595.2018.1438093
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of performance of the 2016 ACR-EULAR classification criteria for primary Sjogren’s syndrome with other sets of criteria in Japanese patients. (2017)
  • Author(s): Tsuboi H., Hagiwara S., Asashima H., Takahashi H., Harota T., Noma H., Umehara H., Kawakami A., Nakamura H., Sano H., Tsubota K., Ozawa Y., Takamura E., Saito I., Inoue H., Nakamura S., Moriyama M., Takeuchi T., Tanaka Y., Hirata S., Mimori T., Matsumoto I., Sumida T.
  • Journal Title: Ann. Rheum. Dis. Volume: 3 Issue: 12 Pages: 2016-210758
  • DOI: 10.1136/annrheumdis-2016-210758
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Sjogren’s syndrome in SATB1 conditional knockout mice (2019)
  • Author(s): Ujjal K Bhawal, Fengzhu Zhang, Yuriko Tanaka, Yumi Ito, Motonari Kondo, Kazumune Arikawa, Koichi Hiratsuka
  • Organizer: 第68回日本口腔衛生学会・総会