| Project/Area Number |
16K11497
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
菅原 俊二 東北大学, 歯学研究科, 教授 (10241639)
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Yukinori
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 金属アレルギー / 樹状細胞 / ニッケルアレルギー / ニッケルイオン結合性細胞 |
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| Outline of Final Research Achievements |
Metal allergies have been classified as Th1-dependent type-IV allergies. The most frequent metal allergen is nickel (Ni). In this study, we analyzed the Ni-binding ability of DCs using the fluorescent metal indicator.
Classical DCs (cDCs) and plasmacytoid DCs were detected in the draining LNs. cDCs were further classified into MHC class II hi CD11c int migratory cDCs and MHC class II int CD11c hi resident cDCs. The Ni-binding ability of the migratory cDCs was significantly higher in skin-draining and submandibular LNs than mesenteric LNs. On the other hand, the Ni-binding ability of the resident cDCs was significantly lower than that of the migratory cDCs, but no significant differences were detected between LNs. Moreover, subcutaneous injection of Ni-treated DCs purified from the skin-draining LNs induced Ni-allergic inflammation in Ni-sensitized mice. These results demonstrate that migratory cDCs in the skin-draining LNs have Ni-binding capability and elicit Ni allergy.
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| Academic Significance and Societal Importance of the Research Achievements |
現在、金属アレルギーに対する対処法としては原因金属の曝露を回避すること以外に確立されていない。しかしながら、日用品から医療器具に至るまで、多種多様な金属製品に囲まれている現在社会において、この対処法を完遂することは困難であり、金属アレルギー患者のQOLを低下させることにもつながる。
本研究で明らかしたNi結合性樹状細胞は、金属アレルギーの発症メカニズムの解明において重要な知見であり、より根本的な治療法・対処法のターゲットにもなり得ると考えられる。
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