Establishment of Analysis of Genetic Diversity and Mutations and Method for Diagnosing Malignancy of Salivary Gland Tumor using Salivary Exosomes
Project/Area Number |
16K11524
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pathobiological dentistry/Dental radiology
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Research Institution | Tokyo Dental College |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | エクソソーム / バイオマーカー / DNA / 腫瘍 / exosome / 唾液 / DNA解析 |
Outline of Final Research Achievements |
This study explored the nature of dsDNA contained in the exosomes of cancer cells. Ultracentrifugation was used to purify the exosomes, and the expression of the exosome markers was confirmed by FACS and Western blot analysis. DNA was extracted from the exosomes, and the size of the DNA was evaluated by Bioanalyzer. TEM observation confirmed the expression of dsDNA in the exosomes. Analysis of dsDNA in the exosome revealed that it reflects a tumor specific gene mutation possessed by the parent cell. In addition, whole exome sequencing indicated that the DNA of the parent cell matches the DNA in the exosomes.
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Academic Significance and Societal Importance of the Research Achievements |
本研究により、癌細胞由来のエクソソームを解析することで、癌細胞の遺伝子情報を得ることができ、診断やその後の治療方針に大変有効な情報を得ることができることがわかった。これにより、血液や唾液といった患者の体液中のエクソソームを解析するだけで、癌の遺伝子情報を踏まえた診断が可能となることも期待できる。
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Platelets Enhance Multiple Myeloma Progression via IL-1β Upregulation2018
Author(s)
Takagi S, Tsukamoto S, Park J, Johnson KE, Kawano Y, Moschetta M, Liu CJ, Mishima Y, Kokubun K, Manier S, Salem KZ, Huynh D, Sacco A, Forward J, Roccaro AM, Battinelli EM, Ghobrial IM.
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Journal Title
Clinical Cancer Research
Volume: 24
Issue: 10
Pages: 2430-2439
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Blocking IFNAR1 inhibits multiple myeloma?driven Treg expansion and immunosuppression2018
Author(s)
Kawano Y, Zavidij O, Park J, Moschetta M, Kokubun K, Mouhieddine TH, Manier S, Mishima Y, Murakami N, Bustoros M, Pistofidis RS, Reidy M, Shen YJ, Rahmat M, Lukyanchykov P, Karreci ES, Tsukamoto S, Shi J, Takagi S, Huynh D, Sacco A, Tai YT, Chesi M, Bergsagel PL, Roccaro AM, Azzi J, Ghobrial IM.
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Journal Title
Journal of Clinical Investigation
Volume: 128
Issue: 6
Pages: 2487-2499
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Inhibition of microRNA-138 enhances bone formation in multiple myeloma bone marrow niche2018
Author(s)
Tsukamoto S, Lovendorf MB, Park J, Salem KZ, Reagan MR, Manier S, Zavidij O, Rahmat M, Huynh D, Takagi S, Kawano Y, Kokubun K, Thrue CA, Nagano K, Petri A, Roccaro AM, Capelletti M, Baron R, Kauppinen S, Ghobrial IM.
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Journal Title
Leukemia
Volume: 32
Issue: 8
Pages: 1739-1750
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Profiling of circulating exosomal miRNAs in patients with Waldenstr?m Macroglobulinemia2018
Author(s)
Bouyssou JM, Liu CJ, Bustoros M, Sklavenitis-Pistofidis R, Aljawai Y, Manier S, Yosef A, Sacco A, Kokubun K, Tsukamoto S, Perilla Glen A, Huynh D, Castillo JJ, Treon SP, Leblond V, Hermine O, Roccaro AM, Ghobrial IM, Capelletti M.
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Journal Title
PLOS ONE
Volume: 13
Issue: 10
Pages: e0204589-e0204589
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] ラット唾液腺創傷部に入れたマトリゲルの影響 in vivoからin vitroでの検討2018
Author(s)
金内 洋光, 小林 史卓, 遠藤 多加史, 外村 岳央, 明石 良彦, 鷲見 正美, 根本 淳, 中條 貴俊, 矢野 尚, 中島 啓, 國分 克寿, 村上 聡, 松坂 賢一, 井上 孝
Organizer
第305回東京歯科大学学会
Related Report
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[Presentation] FGF7はラット唾液腺創傷治癒における幹細胞の細胞増殖と分化を促進させる2018
Author(s)
遠藤 多加史, 小林 史卓, 金内 洋光, 外村 岳央, 明石 良彦, 鷲見 正美, 根本 淳, 中條 貴俊, 中島 啓, 矢野 尚, 國分 克寿, 村上 聡, 松坂 賢一, 井上 孝
Organizer
第305回東京歯科大学学会
Related Report