| Project/Area Number |
16K11658
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Meikai University (2017-2018)
Tokyo Medical University (2016) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
米山 勇哉 杏林大学, 医学部, 助教 (10759799)
近津 大地 東京医科大学, 医学部, 主任教授 (30343122)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 骨再生 / 再生医療 / Tissue Engineering / Jaw Reconstrustion / 骨形成 / 未分化間葉系細胞 / 骨誘導因子 / 生体吸収性材料 / PLGA / ドラックデリバリー / デキサメタゾン / アスコルビン酸 / ベータグリセロリン酸 / プロスタグランジン / 顎骨再建 |
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| Outline of Final Research Achievements |
A biodegradable material with controlled release of growth factors for jaw reconstructions was invented. PLGA, biodegradable polymer, was used as a scaffold material. Dexamethasone, ascorbic acid, and beta-glycerophosphate were dissolved in saline for analysis. Control release of the avobe factors was detected. Dexamethasone was released for 3 hours. Ascorbic acid was released for 3, 12, and 24 hours. Hoewver local concentration was gradually decreased. Beta-glycerophosphate was released for 3, 12, and 24 hours. Hoewver local concentration was gradually decreased.
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| Academic Significance and Societal Importance of the Research Achievements |
顎骨再建において, 多くの骨伝導能を有する材料が使われている. しかし骨誘導能を有し, かつ副作用のない材料は少ない. 本研究では骨が必要な場所へ骨形成を誘導するために, 時間経過とともに適量の骨誘導因子をcontrolled releaseするscaffoldを開発し, その放出局所濃度を測定した. 未分化細胞は骨誘導因子のDex(10-100 nM), AsAc(50 μg/mL), bGP(10 mM)のもとで効率的に骨芽細胞へ分化し増殖するが, 本研究では, PLGAから3種類の骨誘導因子を持続的に放出させることが可能となり, 骨芽細胞の分化・増殖に対し有効であることが示唆された.
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