| Project/Area Number |
16K11698
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tohoku University (2017-2018)
Kyushu Dental College (2016) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
西原 達次 九州歯科大学, 歯学部, 特任教授 (80192251)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 癌 / ソノポレーション / マイクロバブル / ナノバブル / ドラッグデリバリーシステム / 遺伝子導入 |
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| Outline of Final Research Achievements |
Sonoporation is a drug and gene delivery system using ultrasonication that allows the intracellular delivery of foreign molecules that cannot enter cells under normal conditions.In this study, we developed anti-epidermal growth factor receptor (EGFR) antibody-conjugated Microbubbles (EGFR-MBs) and evaluated their capacity to enhance anti-cancer drug toxicity in vitro and in vivo. Bleomycin delivery using sonoporation with EGFR-MBs was significantly more toxic to the cells compared with the other groups.We next examined the effect of EGFR-MBs in a murine squamous cell carcinoma model. Bleomycin delivery by sonoporation with EGFR-MBs exhibited remarkable antitumor activity.
We focused novel platinum nanoparticles-conjugated latex beads, platinum nanocomposite(PtNCP) beads, and investigated the possibility to incorporate novel anti-cancer effect of these combined nanoparticles. PtNCP beads treatment suppressed tumor growth and identified increasing pathological necrotic areas, in vivo.
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| Academic Significance and Societal Importance of the Research Achievements |
舌癌などは、表層から超音波を当てることができ，ソノポレーション法を用いる部位として適している．抗EGFR抗体修飾マイクロバブルは表面が水溶性ポリエチレングリコールで修飾されているため，肝臓などを中心とする細網内皮系組織の貪食細胞に処理されず，血中に長時間循環滞留することができる．バブルに抗EGFR抗体を結合させることにより，より選択的に癌局所へ抗癌物質を集積することができる．これらのバブルを用いれば，ターゲットとなる癌組織にバブルを集積させ，体外からの超音波診断とともに，治療用超音波エネルギーで，非侵襲的に抗癌剤を導入できる．口腔癌の治療を始め，様々な難治性疾患の治療に応用可能である．
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