Development of specific molecular target therapy for oral cancer using new drug delivery system
| Project/Area Number |
16K11698
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tohoku University (2017-2018)
Kyushu Dental College (2016) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
西原 達次 九州歯科大学, 歯学部, 特任教授 (80192251)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 癌 / ソノポレーション / マイクロバブル / ナノバブル / ドラッグデリバリーシステム / 遺伝子導入 |
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| Outline of Final Research Achievements |
Sonoporation is a drug and gene delivery system using ultrasonication that allows the intracellular delivery of foreign molecules that cannot enter cells under normal conditions.In this study, we developed anti-epidermal growth factor receptor (EGFR) antibody-conjugated Microbubbles (EGFR-MBs) and evaluated their capacity to enhance anti-cancer drug toxicity in vitro and in vivo. Bleomycin delivery using sonoporation with EGFR-MBs was significantly more toxic to the cells compared with the other groups.We next examined the effect of EGFR-MBs in a murine squamous cell carcinoma model. Bleomycin delivery by sonoporation with EGFR-MBs exhibited remarkable antitumor activity.
We focused novel platinum nanoparticles-conjugated latex beads, platinum nanocomposite(PtNCP) beads, and investigated the possibility to incorporate novel anti-cancer effect of these combined nanoparticles. PtNCP beads treatment suppressed tumor growth and identified increasing pathological necrotic areas, in vivo.
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| Academic Significance and Societal Importance of the Research Achievements |
舌癌などは、表層から超音波を当てることができ，ソノポレーション法を用いる部位として適している．抗EGFR抗体修飾マイクロバブルは表面が水溶性ポリエチレングリコールで修飾されているため，肝臓などを中心とする細網内皮系組織の貪食細胞に処理されず，血中に長時間循環滞留することができる．バブルに抗EGFR抗体を結合させることにより，より選択的に癌局所へ抗癌物質を集積することができる．これらのバブルを用いれば，ターゲットとなる癌組織にバブルを集積させ，体外からの超音波診断とともに，治療用超音波エネルギーで，非侵襲的に抗癌剤を導入できる．口腔癌の治療を始め，様々な難治性疾患の治療に応用可能である．
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Anticancer effect of novel platinum nanocomposite beads on oral squamous cell carcinoma cells2019
Author(s)
Tanaka M., Okinaga T., Iwanaga K., Matsuo K., Toyono T., Sasaguri M., Ariyoshi W., Tominaga K., Enomoto Y., Matsumura Y., Nishihara, T.
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Journal Title
J Biomed Mater Res B Appl Biomater
Volume: 9999B
Issue: 7
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Application of combination bubble liposomal amphotericin B and sonication has the dramatic effect on oral candidiasis.2017
Author(s)
Yamamoto, M., Iwanaga, K., Okinaga, T., Ariyoshi, W., Tominaga, K., Nishihara, T.
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Journal Title
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology.
Volume: 29
Issue: 3
Pages: 193-197
DOI
Related Report
Peer Reviewed
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[Presentation] Ten-year change of social capital status of oral health in local residents2018
Author(s)
Syungo Watanabe, Kenjiro Iwanaga, Toru Tamahara, Mina Dodo, Wakana Iijima, Mana Igari, Tsubasa Kato, Atsushi Tanaka, Hitoshi Kiyonobu, Naoko Tanda, Takeyoshi Koseki
Organizer
International Symposium for Multimodal Research and Education in IOHS-Liaison 2018
Related Report
Int'l Joint Research
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