Expression of prenyltransferase gene in rubber degrading actinomycete

• Project/Area Number: 16K12631
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental conscious materials and recycle
• Research Institution: Nagaoka University of Technology
• Principal Investigator: Kasai Daisuke 長岡技術科学大学, 工学研究科, 准教授 (80452085)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: ゴム分解細菌 / プレニルトランスフェラーゼ / プレニルトランスフェラーぜ / ポリイソプレン

Outline of Final Research Achievements

Natural rubber from H. brasiliensis is used industrially for tires, seismic isolation rubbers, and medical gloves. The waste of these products have been treated by combustion or landfill processes, which are hazardous to the environment. It is required to find alternative treatment process for rubber waste. In the present study, the characterization of the rubber degradation mechanism of natural rubber degrading actinomycete and the expression of prenyltransferase which has the activity to polymerize isoprene unit, in the rubber degrader were carried out, in order to establish alternative recycle system for rubber waste. When the prenyltransferase gene from Gordonia which was cloned to pNC9503 was introduced into the cells of the rubber degrader, the expression of the gene was found. Further improvement of the gene expression will be needed to develop the recycle system for rubber waste using the rubber degrading actinomycete.

Research Products

• [Journal Article] Identification of Natural Rubber Degradation Gene in Rhizobacter gummiphilus NS21 (2017)
  • Author(s): Kasai, D., Imai, S., Asano, S., Tabata, M., Iijima, S., Kamimura, N., Masai, E., Fukuda, M.
  • Journal Title: Biosci Biotechnol Biochem Volume: 81 Issue: 3 Pages: 614-620
  • DOI: 10.1080/09168451.2016.1263147
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization and functional expression of a rubber degradation gene of a Nocardia degrader from a rubber-processing factory (2017)
  • Author(s): Linh, D.V., Huong, N.L., Tabata, M., Imai, S., Iijima, S., Kasai, D., Anh, T.K., Fukuda, M.
  • Journal Title: J Biosci Bioeng Volume: 123 Issue: 4 Pages: 9-12
  • DOI: 10.1016/j.jbiosc.2016.11.012
  • NAID: 40021178797
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Characterization of natural rubber degradation gene in Rhizobacter gummiphilus NS21 (2016)
  • Author(s): 笠井大輔、今井俊輔、田端理朗、上村直史、政井英司、福田雅夫
  • Organizer: International BioFest 2016
  • Place of Presentation: オーストラリア、メルボルン
  • Year and Date: 2016-10-24
  • Int'l Joint Research
• [Presentation] Characterization of rubber degradation by a Rhodococcus rubber degrader (2016)
  • Author(s): Dao Viet Linh、今井俊輔、田端理朗、笠井大輔、福田雅夫
  • Organizer: International BioFest 2016
  • Place of Presentation: オーストラリア、メルボルン
  • Year and Date: 2016-10-24
  • Int'l Joint Research
• [Presentation] 天然ゴム分解菌Rhizobacter gummiphilus NS21株のポリイソプレン代謝酵素遺伝子群の解明 (2016)
  • Author(s): 笠井大輔、今井俊輔、小黒健太、田端理朗、上村直史、政井英司、福田雅夫
  • Organizer: 環境環境バイオテクノロジー学会2016年度大会
  • Place of Presentation: 広島
  • Year and Date: 2016-06-13