| Project/Area Number |
16K12712
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
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| Research Institution | Kyoto Women's University |
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Principal Investigator |
Hatta Hajime 京都女子大学, 家政学部, 教授 (00309056)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | IgY / 黄色ぶどう球菌 / アトピー性皮膚炎 / 増殖抑制 / 鶏卵抗体 / 黄色ブドウ球菌 / 表皮ブドウ球菌 / λーカライギナン / 免疫注射 / アジュバンド |
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| Outline of Final Research Achievements |
S. aureus (SA) has been considered one of causative bacteria of atopic dermatitis. The purpose of this study is to evaluate the growth inhibitory effect of anti-SA IgY against S. aureus. This study might lead to a novel application of an anti-SA IgY for prevention of atopic dermatitis using a skin care cream containing an anti-SA IgY antibody. Control IgY or anti-SA IgY was adjusted to a concentration of 0-10 mg/ml, and each was mixed with SA solution at a volume ratio of 1: 1. Bacterial growth was monitored by absorbance at 600 nm or by ATP chemiluminescence assay at 0, 4, 6, 8, 12 and 24 hours incubation at 37 ° C. It was found that the growth inhibitory effect of anti-SA IgY showed delaying the lag phase of SA growth curve for 4 hours compared to that of Control IgY. Four hours of bacteriostatic on SA growth could be a prevention measure against atopic dermatitis in using a skin care cream containing an anti-SA IgY antibody.
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| Academic Significance and Societal Importance of the Research Achievements |
アトピー体質の皮膚常在細菌は表皮ブドウ球菌（SE）より黄色ブドウ球菌(Staphylococcus aureus：SA)が優勢であり、それが産生する毒素が皮膚の炎症を誘発する。本研究では産卵鶏をSA菌体抗原で過免疫し、その鶏卵卵黄から精製した抗 SA IgY抗体（濃度10mg/ml ）がSA菌の増殖誘導期を約４時間遅延させることを示した。このことから、さらに研究が必要であるが、抗SA IgY抗体を配合したクリームを皮膚に４時間毎に塗布することで、SA菌の増殖を抑制しアトピー性皮膚炎の予防に利用できる可能性が期待される。
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