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Novel mechanism how hypothalamus senses the lipid of the blood.

Research Project

Project/Area Number 16K12735
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionTohoku University

Principal Investigator

Owda Yuji  東北大学, 医学系研究科, 教授 (20292211)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsマウス / 視床下部 / 弓状核 / グリア細胞 / 脂質 / レプチン / 高脂肪食 / アストロサイト / オリゴデンドロサイト
Outline of Final Research Achievements

The hypothalamus is involved in the regulation of food intake and energy homeostasis. However, the molecular mechanisms involved in these activities remain largely unknown. In this study, we examined in detail the localization of fatty acid-binding protein 7 (FABP7) in the hypothalamic ARC. Immunohistochemistry revealed that FABP7+ cells are NG2+ or GFAP+ in the ARC. In mice fed a high-fat diet, weight gain and food intake were lower in FABP7 KO mice than in wild-type (WT) mice. FABP7 KO mice also had lower food intake and weight gain after a single injection of leptin, and we consistently confirmed that the number of pSTAT3+ cells in the ARC indicated that the leptin-induced activation of neurons was significantly more frequent in FABP7 KO mice than in WT mice.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Glial Fatty Acid-Binding Protein 7 (FABP7) Regulates Neuronal Leptin Sensitivity in the Hypothalamic Arcuate Nucleus2018

    • Author(s)
      Yasumoto Yuki、Miyazaki Hirofumi、Ogata Masaki、Kagawa Yoshiteru、Yamamoto Yui、Islam Ariful、Yamada Tetsuya、Katagiri Hideki、Owada Yuji
    • Journal Title

      Molecular Neurobiology

      Volume: - Issue: 12 Pages: 9016-9028

    • DOI

      10.1007/s12035-018-1033-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Normal sleep requires the astrocyte brain-type fatty acid binding protein FABP7.2017

    • Author(s)
      Gerstner JR, Perron IJ, Riedy SM, Yoshikawa T, Kadotani H, Owada, Y, Van Dongen HPA, Galante RJ, Dickinson K, Yin JCP, Pack AI, Frank MG.
    • Journal Title

      Science Adv

      Volume: 3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases2017

    • Author(s)
      Tetsuro Matsuhashi, Takeya Sato,(間30名),Yuji Owada, 他9名
    • Journal Title

      EBioMedicine

      Volume: 20 Pages: 27

    • DOI

      10.1016/j.ebiom.2017.05.016

    • NAID

      120007134536

    • URL

      https://pure.teikyo.jp/en/publications/b6fee86c-4659-43f6-98ca-a57629957a0b

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microglia-derived neuregulin expression in psychiatric disorders.2017

    • Author(s)
      Ikawa D, Makinodan M, Iwata K, Ohgidani M, Kato TA, Yamashita Y, Yamamuro K, Kimoto S, Toritsuka M, Yamauchi T, Fukami SI, Yoshino H, Okumura K, Tanaka T, Wanaka A, Owada Y, Tsujii M, Sugiyama T, Tsuchiya K, Mori N, Hashimoto R, Matsuzaki H, Kanba S, Kishimoto T.
    • Journal Title

      Brain Behav Immun.

      Volume: 61 Pages: 375-385

    • DOI

      10.1016/j.bbi.2017.01.003

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultrastructural histomeric evidence for expansion of sustentacular cell envelop in response to hypersecretion of adrenal chromaffin cells in mice.2017

    • Author(s)
      Pakkarato S, Thoungseabyoun W, Tachow A, Rawangwong A, Kagawa Y, Owada Y, Kondo H, Hipkaeo W.
    • Journal Title

      Anat Sci Int

      Volume: 印刷中 Issue: 1 Pages: 75-81

    • DOI

      10.1007/s12565-016-0370-x

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Motor training promotes both synaptic and intrinsic plasticity of layer II/III pyramidal neurons in the primary motor cortex.2016

    • Author(s)
      Kida H, Tsuda Y, Ito N, Yamamoto Y, Owada Y, Kamiya Y, Mitsushima D.
    • Journal Title

      Cerebral Cortex

      Volume: in press Issue: 8 Pages: 3494-507

    • DOI

      10.1093/cercor/bhw134

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Fatty Acid-Binding Protein 5 at the Blood-Brain Barrier Regulates Endogenous Brain Docosahexaenoic Acid Levels and Cognitive Function.2016

    • Author(s)
      Pan Y, Short JL, Choy KH, Zeng AX, Marriott PJ, Owada Y, Scanlon MJ, Porter CJ, Nicolazzo JA.
    • Journal Title

      J Neurosci.

      Volume: 36 Issue: 46 Pages: 11755-11767

    • DOI

      10.1523/jneurosci.1583-16.2016

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] FABP7は視床下部アストロサイトにおけるレプチン感受性を調節し、高脂肪食摂取量を抑制する2017

    • Author(s)
      安本有希, 宮崎啓史, 大和田祐二
    • Organizer
      第122回 日本解剖学会総会
    • Place of Presentation
      長崎市 長崎大学
    • Year and Date
      2017-03-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 細胞核内FABP7はcaveolin-1遺伝子発現をエピジェネティックに制御する2017

    • Author(s)
      香川慶輝, Ariful Islam, 尾形雅君, 大和田祐二
    • Organizer
      第122回 日本解剖学会総会
    • Place of Presentation
      長崎市 長崎大学
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] マクロファージの抗炎症性機能分化における細胞内代謝の変化とFABP7の関与2017

    • Author(s)
      宮崎啓史, 宍戸愛, 香川慶輝, 安本有希, 南都文香, 大和田祐二
    • Organizer
      日本解剖学会 第63回東北・北海道連合支部学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ミトコンドリア呼吸鎖複合体I構成蛋白質Ndufs4がアストロサイトのミトコンドリア機能におよぼす動的変化および影響2017

    • Author(s)
      南都文香, Angelina Misiou, Subrata Kumar Shil, 阿部高明, 大和田祐二
    • Organizer
      日本解剖学会 第63回東北・北海道連合支部学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Role of FABP7 in astrocytes and its possible association with human psychiatric diseases2017

    • Author(s)
      Yuji Owada
    • Organizer
      Protein Misfolding Diseases and Therapy 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] FABP3 in the anterior cingulate cortex mediates methylation status of GAD67 promotor region2017

    • Author(s)
      Yuji Owada, Yui Yamamoto, Hiroyuki Kida, Yuchio Yanagawa, Dai Mitsushima, Kohji Fukunaga
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 核内FABP7はエピジェネティックなcaveolin-1の発現制御に関与する2016

    • Author(s)
      香川慶輝, Ariful Islam, 尾形雅君, 大和田祐二
    • Organizer
      第39回 日本分子生物学会年会
    • Place of Presentation
      横浜市 パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 視床下部グリアに発現するFABP7のレプチン感受性と摂食行動における機能2016

    • Author(s)
      安本有希, 大和田祐二
    • Organizer
      第62回 日本解剖学会 東北・北海道連合支部学術集会
    • Place of Presentation
      帯広市 帯広畜産大学
    • Year and Date
      2016-09-03
    • Related Report
      2016 Research-status Report
  • [Presentation] Possible role of hypothalamic FABP7 in the cotrol of glial cell proliferation and leptin sensitivity2016

    • Author(s)
      Yasumoto Y and Owada Y
    • Organizer
      Cold Spring Harbor Laboratory Meeting - Glia in Health and Disease
    • Place of Presentation
      Cold Spring Harbor Laboratoy, NY, USA
    • Year and Date
      2016-07-30
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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