Drug screening using a newly developed cell assay
| Project/Area Number |
16K12872
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Osaka University |
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Principal Investigator |
Deguchi Shinji 大阪大学, 基礎工学研究科, 教授 (30379713)
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| Research Collaborator |
Matsui Tsubasa S.
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細胞バイオメカニクス / 細胞収縮力 / メカノミクス / ドラッグリポジショニング / ドラッグ・リポジショニング / 創薬 / メカノーム |
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| Outline of Final Research Achievements |
Cells regulate their function according to the contractile force that the individual cells generate. However, it remains unclear regarding the detailed relationship between the cell functions and contractile force because of the lack of methodologies allowing comprehensive analysis on the relationship. Here, we have developed a new method that allows us to quantitatively evaluate the cellular contractile force with a high-throughput analysis capability. We demonstrated with the new system that some molecular regulators are found to be effective for modulating the cellular contractile force.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞の「収縮」に関する研究と言えばこれまで主に筋肉（骨格筋、心筋、平滑筋）に関するものが主なものでした。しかし昨今、「非筋」細胞における微小な収縮が、広範な細胞機能の調節に重要な役割を果たしていることが明らかにされています。本研究では非筋細胞の収縮の程度を高い実験効率で評価できる新しい装置の開発を行いました。この装置を用いた実験により、非筋細胞の収縮を制御する薬剤の同定を行いました。これにより非筋細胞内の収縮能の調節およびそれに依存した諸機能の分子機構の解明につながることが期待されます。
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation2017
Author(s)
Ichikawa, T., Kita, M., Matsui, T.S., Ichikawa-Nagasato, A., Araki, T., Chiang, S.H., Sezaki, T., Kimura, Y., Ueda, K., Deguchi, S., Saltiel, A.R., Kioka, N.
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Journal Title
Journal of Cell Science
Volume: 130
Issue: 20
Pages: 3517-3531
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Cellular force assay detects altered contractility caused by a nephritis-associated mutation in nonmuscle myosin IIA2017
Author(s)
Fukuda, S.P., Matsui, T.S., Ichikawa, T., Furukawa, T., Kioka, N., Fukushima, S., Deguchi, S.
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Journal Title
Development, Growth & Differentiation
Volume: 59
Issue: 5
Pages: 423-433
DOI
Related Report
Peer Reviewed
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[Journal Article] Conformational Plasticity of JRAB/MICAL-L2 Provides 'Law and Order'2016
Author(s)
A. Sakane, S. Yoshizawa, M. Nishimura, Y. Tsuchiya, N. Matsushita, K. Miyake, K. Horikawa, I. Imoto, C. Mizuguchi, H. Saito, T. Ueno, S. Matsushita, H. Haga, S. Deguchi, K. Mizuguchi, H.
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Journal Title
Molecular Biology of the Cell
Volume: 27
Issue: 20
Pages: 3095-3108
DOI
Related Report
Peer Reviewed / Open Access
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