• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Drug screening using a newly developed cell assay

Research Project

Project/Area Number 16K12872
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionOsaka University

Principal Investigator

Deguchi Shinji  大阪大学, 基礎工学研究科, 教授 (30379713)

Research Collaborator Matsui Tsubasa S.  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords細胞バイオメカニクス / 細胞収縮力 / メカノミクス / ドラッグリポジショニング / ドラッグ・リポジショニング / 創薬 / メカノーム
Outline of Final Research Achievements

Cells regulate their function according to the contractile force that the individual cells generate. However, it remains unclear regarding the detailed relationship between the cell functions and contractile force because of the lack of methodologies allowing comprehensive analysis on the relationship. Here, we have developed a new method that allows us to quantitatively evaluate the cellular contractile force with a high-throughput analysis capability. We demonstrated with the new system that some molecular regulators are found to be effective for modulating the cellular contractile force.

Academic Significance and Societal Importance of the Research Achievements

細胞の「収縮」に関する研究と言えばこれまで主に筋肉(骨格筋、心筋、平滑筋)に関するものが主なものでした。しかし昨今、「非筋」細胞における微小な収縮が、広範な細胞機能の調節に重要な役割を果たしていることが明らかにされています。本研究では非筋細胞の収縮の程度を高い実験効率で評価できる新しい装置の開発を行いました。この装置を用いた実験により、非筋細胞の収縮を制御する薬剤の同定を行いました。これにより非筋細胞内の収縮能の調節およびそれに依存した諸機能の分子機構の解明につながることが期待されます。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (28 results)

All 2019 2018 2017 2016 Other

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) (of which Int'l Joint Research: 9 results,  Invited: 3 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Keratin-binding ability of the N-terminal Solo domain of Solo is critical for its function in cellular mechanotransduction2019

    • Author(s)
      Fujiwara, S., Matsui, T. S., Ohashi, K., Mizuno, K., and Deguchi, S.
    • Journal Title

      Genes Cells

      Volume: 印刷中 Issue: 5 Pages: 390-402

    • DOI

      10.1111/gtc.12682

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatially selective MRLC regulation is absent in dedifferentiated vascular smooth muscle cells but is partially induced by fibronectin and Klf42019

    • Author(s)
      Matsui, T.S., Deguchi, S.
    • Journal Title

      American Journal of Physiology Cell Physiology

      Volume: 316 Issue: 4 Pages: C509-C521

    • DOI

      10.1152/ajpcell.00251.2017

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transgelin-1 (SM22α) interacts with actin stress fibers and podosomes in smooth muscle cells without using its actin binding site2018

    • Author(s)
      Matsui, T.S., Ishikawa, A., Deguchi, S.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 505 Issue: 3 Pages: 879-884

    • DOI

      10.1016/j.bbrc.2018.09.176

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deformable 96-well cell culture plate compatible with high-throughput screening platforms2018

    • Author(s)
      Matsui, T.S., Wu, H., Deguchi, S.
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 9 Pages: e0203448-e0203448

    • DOI

      10.1371/journal.pone.0203448

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Solo, a RhoA-targeting guanine nucleotide exchange factor, is critical for hemidesmosome formation and acinar development in epithelial cells2018

    • Author(s)
      Sachiko Fujiwara, Tsubasa S. Matsui, Kazumasa Ohashi, Shinji Deguchi, and Kensaku Mizuno
    • Journal Title

      PLOS ONE

      Volume: 印刷中 Issue: 4 Pages: e0195124-e0195124

    • DOI

      10.1371/journal.pone.0195124

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation2017

    • Author(s)
      Ichikawa, T., Kita, M., Matsui, T.S., Ichikawa-Nagasato, A., Araki, T., Chiang, S.H., Sezaki, T., Kimura, Y., Ueda, K., Deguchi, S., Saltiel, A.R., Kioka, N.
    • Journal Title

      Journal of Cell Science

      Volume: 130 Issue: 20 Pages: 3517-3531

    • DOI

      10.1242/jcs.200691

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cellular force assay detects altered contractility caused by a nephritis-associated mutation in nonmuscle myosin IIA2017

    • Author(s)
      Fukuda, S.P., Matsui, T.S., Ichikawa, T., Furukawa, T., Kioka, N., Fukushima, S., Deguchi, S.
    • Journal Title

      Development, Growth & Differentiation

      Volume: 59 Issue: 5 Pages: 423-433

    • DOI

      10.1111/dgd.12379

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] New wrinkling substrate assay reveals traction force fields of leader and follower cells undergoing collective migration2017

    • Author(s)
      Yokoyama, S., Matsui, T.S., Deguchi, S.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 482 Issue: 4 Pages: 975-979

    • DOI

      10.1016/j.bbrc.2016.11.142

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Microcontact Peeling: A Cell Micropatterning Technique for Circumventing Direct Adsorption of Proteins to Hydrophobic PDMS2017

    • Author(s)
      Yokoyama, S., Matsui, T.S., Deguchi, S.
    • Journal Title

      Current Protocols in Cell Biology

      Volume: 75

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tensile strength of oxygen plasma-created surface layer of PDMS2017

    • Author(s)
      Ohishi, T., Noda, H., Matsui, T.S., Jile, H., Deguchi, S.
    • Journal Title

      Journal of Micromechanics and Microengineering

      Volume: 27 Pages: 015015-015015

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Conformational Plasticity of JRAB/MICAL-L2 Provides 'Law and Order'2016

    • Author(s)
      A. Sakane, S. Yoshizawa, M. Nishimura, Y. Tsuchiya, N. Matsushita, K. Miyake, K. Horikawa, I. Imoto, C. Mizuguchi, H. Saito, T. Ueno, S. Matsushita, H. Haga, S. Deguchi, K. Mizuguchi, H.
    • Journal Title

      Molecular Biology of the Cell

      Volume: 27 Issue: 20 Pages: 3095-3108

    • DOI

      10.1091/mbc.e16-05-0332

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] A cellular contractility monitoring system allowing for high- throughput and long-term analysis2018

    • Author(s)
      T.S. Matsui, S. Deguchi
    • Organizer
      MHS 2018 (International Symposium on Micro-NanoMechatronics and Human Science)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rho-signaling organizes hemidesmosomal cell-substrate adhesion and acinar morphogenesis of epithelial cells through controlling mechanotransduction2018

    • Author(s)
      S. Fujiwara, T.S. Matsui, K. Ohashi, T.M. Magin, K. Mizuno, S. Deguchi
    • Organizer
      International Meeting of the German Society for Cell Biology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High-throughput detection of cellular traction forces for screening of drugs and regulatory genes2018

    • Author(s)
      Deguchi, S.
    • Organizer
      Emerging Technologies in Mechanical Engineering 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Stress fibers exhibit unique contractile properties distinct from those of myofibrils2018

    • Author(s)
      Deguchi, S., Matsui, T.S
    • Organizer
      8th World Congress of Biomechanics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hemidesmosomal cell-substrate adhesion and acinar morphogenesis of epithelial cells are organized by Rho-signaling through controlling mechanotransduction2018

    • Author(s)
      S. Fujiwara, T. S. Matsui, K. Ohashi, K. Mizuno, S. Deguchi
    • Organizer
      8th World Congress of Biomechanics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rho-signaling plays crucial roles for cellular mechanotransduction and regulates actin and intermediate filament cytoskeletal reorganization2018

    • Author(s)
      S. Fujiwara, H. Abiko, T. S. Matsui, K. Ohashi, K. Mizuno, S. Deguchi
    • Organizer
      8th World Congress of Biomechanics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Isometric contractile properties of individual stress fibers2018

    • Author(s)
      Deguchi, S.
    • Organizer
      8th World Congress of Biomechanics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] スループットの高い細胞トラクションフォースアッセイの開発2017

    • Author(s)
      出口 真次,松井 翼,田中 裕一郎,呼格吉楽
    • Organizer
      第29回バイオエンジニアリング講演会
    • Place of Presentation
      ウインクあいち
    • Year and Date
      2017-01-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Detecting endogenous forces of cells subjected to mutations and drugs2017

    • Author(s)
      S. Deguchi
    • Organizer
      第76回 日本癌学会学術総会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Local geometry sensing by individual focal adhesions2017

    • Author(s)
      Shinji Deguchi, Sho Yokoyama, Tsubasa S. Matsui, Taiki Ohishi
    • Organizer
      2017 Cellular and Molecular Bioengineering Annual Conference
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 個々の細胞が発生する力を高効率に定量化するシステムの開発2016

    • Author(s)
      松井 翼, 出口 真次
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] ミオシン調節軽鎖のリン酸化は個々の細胞が発生する収縮力の大きさに相関はあるか?2016

    • Author(s)
      出口 真次, 松井 翼, 倉賀野 正弘, 高橋 正行
    • Organizer
      第68回日本細胞生物学会大会
    • Place of Presentation
      京都テルサ
    • Year and Date
      2016-06-15
    • Related Report
      2016 Research-status Report
  • [Book] 細胞収縮力アッセイ in 月刊「細胞」2019

    • Author(s)
      出口 真次,松井 翼,市川 尚文,木岡 紀幸
    • Total Pages
      4
    • Publisher
      北隆館 ニューサイエンス社
    • Related Report
      2018 Annual Research Report
  • [Book] 細胞収縮力のアッセイ - メカノバイオロジー研究のツール in Precision Medicine プレシジョンメディシンと創薬2018

    • Author(s)
      出口 真次,松井 翼,市川 尚文,木岡 紀幸
    • Total Pages
      4
    • Publisher
      北隆館 ニューサイエンス社
    • Related Report
      2018 Annual Research Report
  • [Remarks] 大阪大学・出口研

    • URL

      http://mbm.me.es.osaka-u.ac.jp

    • Related Report
      2018 Annual Research Report
  • [Remarks] 大阪大学大学院基礎工学研究科・出口研HP

    • URL

      http://mbm.me.es.osaka-u.ac.jp

    • Related Report
      2017 Research-status Report
  • [Remarks] 大阪大学・出口研究室ホームページ

    • URL

      http://mbm.me.es.osaka-u.ac.jp

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi