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Development of a microchip for capture and disruption of extracellular vesicles toward cancer point-of-care diagnosis

Research Project

Project/Area Number 16K12901
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTokyo University of Science

Principal Investigator

Ishihara Ryo  東京理科大学, 基礎工学部材料工学科, 助教 (30633507)

Co-Investigator(Kenkyū-buntansha) 中島 忠章  東京理科大学, 基礎工学部生物工学科, 助教 (40631213)
Co-Investigator(Renkei-kenkyūsha) KIKUCHI Akihiko  東京理科大学, 基礎工学部 材料工学科, 教授 (40266820)
TOMOOKA Yasuhiro  東京理科大学, 基礎工学部 生物工学科, 教授 (10197949)
MAEDA Mizuo  理化学研究所, 前田バイオ工学研究室, 主任研究員 (10165657)
HOSOKAWA Kazuo  理化学研究所, 前田バイオ工学研究室, 専任研究員 (00373366)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords表面機能化自律駆動マイクロチップ / 放射線グラフト重合法 / 捕捉 / その場診断 / がん / 細胞外ベシクル / 放射線グラフと重合法 / グラフト重合法 / マイクロチップ
Outline of Final Research Achievements

Extracellular vesicles (EVs), which are secreted from cells to body fluids, are expected as new biomarkers because they have information of the parent cells. In this study, to use the information of the EVs for a cancer point-of-care diagnosis, we have been developed microchips for capture and disruption of the EVs. We immobilized antibody, which specifically binds to membrane protein on EVs, to inner surfaces of microchannels of a microchip modified by radiation-induced graft polymerization. Thus, a surface-functionalized power-free (SF-PF) microchip for EV capture was obtained. On the SF-PF microchip, easy and rapid EV capture was demonstrated. These results were published in journals and newspapers. In addition, we also received conference awards. Furthermore, EV disruption on a polymer chain grafted microchannel inner surfaces was also demonstrated. Improvements and integration of the above technologies would contribute to the establishment of a cancer point-of-care diagnosis.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (27 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (22 results) (of which Int'l Joint Research: 7 results,  Invited: 1 results)

  • [Journal Article] High-resolution separation of neodymium and dysprosium ions utilizeing extractant-impregnated graft-type particles2018

    • Author(s)
      S. Uchiyama, T. Sasaki, R. Ishihara, K. Fujiwara, T. Sugo, D. Umeno, K. Saito
    • Journal Title

      J. Chromatogr. A

      Volume: 1533 Pages: 10-16

    • DOI

      10.1016/j.chroma.2017.11.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ポリジメチルシロキサンの気体溶解性を利用した自律駆動型マイクロ流体チップ2018

    • Author(s)
      石原量,猪股祥子,菊池明彦
    • Journal Title

      ぶんせき

      Volume: 1 Pages: 21-27

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of a Surface-functionalized Power-free PDMS Microchip for MicroRNA Detection Utilizing Electron Beam-induced Graft Polymerization2017

    • Author(s)
      R. Ishihara, Y. Uchino, K. Hosokawa, M. Maeda, A. Kikuchi
    • Journal Title

      Analytical Sciences

      Volume: 33 Issue: 2 Pages: 197-202

    • DOI

      10.2116/analsci.33.197

    • NAID

      130005316213

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Rapid and Easy Extracellular Vesicle Detection on a Surface-Functionalized Power-Free Microchip toward Point-of-Care Diagnostics2017

    • Author(s)
      R. Ishihara, T. Nakajima, Y. Uchino, A. Katagiri, K. Hosokawa, M. Maeda, Y. Tomooka, A. Kikuchi
    • Journal Title

      ACS Omega

      Volume: 2 Issue: 10 Pages: 6703-6707

    • DOI

      10.1021/acsomega.7b01147

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] その場検査のための表面機能化自律駆動マイクロチップによるバイオマーカーの検出2017

    • Author(s)
      石原量,猪股祥子,菊池明彦
    • Journal Title

      Colloid & Interface Communication

      Volume: 42 Pages: 30-33

    • NAID

      40021626102

    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞外ベシクル高感度検出のための表面機能化自律駆動マイクロチップの設計2018

    • Author(s)
      石原 量, 片桐明日香,中島忠章,細川和生, 前田瑞夫, 友岡康弘,菊池明彦
    • Organizer
      第67回高分子学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 表面機能化自律駆動マイクロチップを用いた細胞外ベシクルの高感度検出2018

    • Author(s)
      片桐明日香, 石原量, 中島忠章,細川和生, 前田瑞夫, 友岡康弘, 菊池明彦
    • Organizer
      第35回高分子学会千葉地域活動若手セミナー
    • Related Report
      2017 Annual Research Report
  • [Presentation] UVグラフト重合を利用した表面機能化自律駆動マイクロチップによるmiRNAの特異検出2018

    • Author(s)
      猪股祥子, 石原量,細川和生, 前田瑞夫, 菊池明彦
    • Organizer
      第35回高分子学会千葉地域活動若手セミナー
    • Related Report
      2017 Annual Research Report
  • [Presentation] がん特異的な細胞外ベシクル検出のための表面機能化自律駆動マイクロチップの作製2018

    • Author(s)
      松井崚,石原量, 中島忠章, 片桐明日香,細川和生, 前田瑞夫, 友岡康弘, 菊池明彦
    • Organizer
      UVグラフト重合を利用した表面機能化自律駆動マイクロチップによるmiRNAの特異検出
    • Related Report
      2017 Annual Research Report
  • [Presentation] 表面機能化自律駆動マイクロ流体チップを用いたMicroRNAの複数種類同時検出2018

    • Author(s)
      喜種椋一,石原 量,秋山好嗣,猪股祥子,細川和生, 前田瑞夫,菊池明彦
    • Organizer
      第35回高分子学会千葉地域活動若手セミナー
    • Related Report
      2017 Annual Research Report
  • [Presentation] がん特異的な細胞外ベシクルの検出をめざしたマイクロチップの作製2018

    • Author(s)
      松井崚,石原 量, 片桐明日香,中島忠章,細川和生, 前田瑞夫, 友岡康弘,菊池明彦
    • Organizer
      第67回高分子学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] がんのその場診断のためのバイオマーカーの検出2017

    • Author(s)
      石原 量
    • Organizer
      セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Simple Extracellular Vesicle Detection on a Portable Microfluidic Chip toward Point-of-Care Diagnosis2017

    • Author(s)
      R. Ishihara, T. Nakajima, A. Katagiri, Y. Uchino, K. Hosokawa, M. Maeda, Y. Tomooka, A. Kikuchi
    • Organizer
      The 15th International Conference on Advanced Materials (IUMRS-ICAM 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞外ベシクルの高感度検出のための表面機能化自律駆動マイクロチップの作製2017

    • Author(s)
      石原 量, 中島忠章,片桐明日香,細川和生, 前田瑞夫, 友岡康弘,菊池明彦,
    • Organizer
      第39回日本バイオマテリアル学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] UVグラフト重合法を利用して機能化した自律駆動マイクロチップによる細胞外ベシクルの高感度検出2017

    • Author(s)
      石原 量, 中島忠章,片桐明日香,細川和生, 前田瑞夫, 友岡康弘,菊池明彦
    • Organizer
      第27回日本MRS年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Easy Extracellular Vesicle Detection on a Surface-Functionalized Power-Free Microchip2017

    • Author(s)
      R. Ishihara, T. Nakajima, A. Katagiri, Y. Uchino, K. Hosokawa, M. Maeda, Y. Tomooka, A. Kikuchi
    • Organizer
      International Society for Extracellular Vesicles (ISEV2017-Annual Meeting)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Preparation of a Surface-Functionalized Power-Free Microchip for Extracellular Vesicle Detection,2017

    • Author(s)
      A. Katagiri, R. Ishihara, T. Nakajima, Y. Uchino, K. Hosokawa, M. Maeda, A. Kikuchi
    • Organizer
      The 15th International Conference on Advanced Materials (IUMRS-ICAM 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sensitive MicroRNA Detection on a Power-Free Microfluidic Chip Prepared by UV-Induced Graft Polymerization2017

    • Author(s)
      S. Inomata, R. Ishihara, Y. Uchino, K. Hosokawa, M. Maeda, A. Kikuchi
    • Organizer
      The 15th International Conference on Advanced Materials (IUMRS-ICAM 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] UVグラフト重合によって機能化した自律駆動マイクロチップによるmicroRNAの検出2017

    • Author(s)
      猪股祥子,石原 量,内野斐隆,細川和生,前田瑞夫,菊池明彦
    • Organizer
      第66回高分子学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 表面機能化自律駆動マイクロチップを用いた細胞外ベシクルの簡易検出2017

    • Author(s)
      片桐明日香,石原 量,中島忠章,内野斐隆,細川和生,前田瑞夫,友岡康弘,菊池明彦
    • Organizer
      第66回高分子学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞外ベシクル検出用の表面機能化自律駆動マイクロチップの高感度化2017

    • Author(s)
      片桐明日香,石原 量, 中島忠章,細川和生, 前田瑞夫, 友岡康弘,菊池明彦
    • Organizer
      第27回日本MRS年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] miRNA検出のためのUVグラフト重合を利用した表面機能化自律駆動マイクロチップの作製2017

    • Author(s)
      猪股祥子,石原 量,内野斐隆,細川和生, 前田瑞夫,菊池明彦
    • Organizer
      第27回日本MRS年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 表面機能化自律駆動マイクロチップを用いた細胞外ベシクルの簡易検出2017

    • Author(s)
      片桐明日香, 石原量, 中島忠章, 内野斐隆, 細川和生, 前田瑞夫, 友岡康弘, 菊池明彦
    • Organizer
      第34回高分子学会千葉地域活動若手セミナー
    • Place of Presentation
      東京・東京理科大学葛飾キャンパス
    • Related Report
      2016 Research-status Report
  • [Presentation] Extracellular Vesicle Detection on a Power-Free Microchip Functionalized by Electron Beam Grafting2016

    • Author(s)
      Y. Uchino, R. Ishihara, T. Nakajima, K. Hosokawa, M. Maeda, Y. Tomooka, A. Kikuchi
    • Organizer
      3rd International Conference on Biomaterials Science in Tokyo (ICBS2016)
    • Place of Presentation
      Ito Hall at The University of Tokyo, Tokyo, Japan
    • Year and Date
      2016-11-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] その場診断のための表面機能化自律駆動マイクロチップによる細胞外ベシクルの検出2016

    • Author(s)
      石原 量, 中島忠章,片桐明日香,内野斐隆,細川和生, 前田瑞夫, 友岡康弘,菊池明彦
    • Organizer
      日本バイオマテリアル学会 シンポジウム2016
    • Place of Presentation
      福岡・福岡国際会議場
    • Year and Date
      2016-11-21
    • Related Report
      2016 Research-status Report
  • [Presentation] Extracellular Vesicle Detection on a Surface-Functionalized Power-Free Microchip toward Point-of-Care Cancer Diagnosis2016

    • Author(s)
      R. Ishihara, T. Nakajima, Y. Uchino, K. Hosokawa, M. Maeda, Y. Tomooka, A. Kikuchi
    • Organizer
      Liquid Biopsies and Minimally-Invasive Diagnostics 2016
    • Place of Presentation
      Marriott Mission Valley, San Diego, California, USA
    • Year and Date
      2016-09-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Simple Inner-Surface Modification of a PDMS Microchip by Radiation-Induced Graft Polymerization for Extracellular Vesicle Detection toward Point-of-Care Diagnosis2016

    • Author(s)
      R. Ishihara, T. Nakajima, Y. Uchino, K. Tanabe, K. Hosokawa, M. Maeda, Y. Tomooka, A. kikuchi
    • Organizer
      World Biomaterials Congress (WBC 2016)
    • Place of Presentation
      Palais des Congres de Montreal, Montreal, Canada
    • Year and Date
      2016-05-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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