Does lactate facilitate regeneration of injured skeletal muscle?

• Project/Area Number: 16K12942
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Toyohashi Sozo University
• Principal Investigator: OHNO Yoshitaka 豊橋創造大学, 保健医療学部, 講師 (80440808)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 骨格筋 / 細胞・組織 / リハビリテーション

Outline of Final Research Achievements

The purpose of this study was to investigate the effects of lactate on the regeneration of injured skeletal muscle. Cardiotoxin (CTX) was injected into mouse tibialis anterior (TA) muscle. Oral administration of sodium lactate increased the muscle weight and number of Pax7-positive nuclei in CTX-injected TA muscle. Mouse myoblast C2C12 cells were differentiated to form myotubes. Lactate was administered to the differentiation medium. Lactate administration increased the diameter of C2C12 myotubes in a dose-dependent manner. Lactate administration also increased myonuclei number and upregulated the p42/44 extracellular signal-regulated kinase-1/2 (Erk1/2) signaling. These observations suggest that lactate may have a positive effect on the regeneration of injured skeletal muscle.

Research Products

• [Journal Article] Potential involvement of dietary advanced glycation end products in impairment of skeletal muscle growth and muscle contractile function in mice. (2017)
  • Author(s): Egawa T, Tsuda S, Goto A, Yokoyama S, Ohno Y, Goto K, Hayashi T.
  • Journal Title: Br J Nutr. Volume: 117 Issue: 1 Pages: 21-29
  • DOI: 10.1017/s0007114516004591
  • NAID: 120006305928
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Suppression of myostatin stimulates regenerative potential of injured antigravitational soleus muscle in mice under unloading condition (2016)
  • Author(s): Ohno, Y., Matsuba, Y., Hashimoto, N., Sugiura, T., Ohira, Y., Yoshioka, T., Goto, K.
  • Journal Title: International Journal of Medical Sciences Volume: 13 Issue: 9 Pages: 680-685
  • DOI: 10.7150/ijms.16267
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Heat shock transcription factor 1-associated expression of slow myosin heavy chain in mouse soleus muscle in response to unloading with or without reloading (2016)
  • Author(s): Yokoyama S, Ohno Y, Egawa T, Yasuhara K, Nakai A, Sugiura T, Ohira Y, Yoshioka T, Okita M, Origuchi T, Goto K
  • Journal Title: Acta Physiol (Oxf) Volume: 217 Issue: 4 Pages: 325-337
  • DOI: 10.1111/apha.12692
  • Peer Reviewed / Open Access
• [Journal Article] Caffeine affects myotube size as well as regulates protein degradation and protein synthesis pathways in C2C12 skeletal muscle cells. (2016)
  • Author(s): Egawa T, Ohno Y, Goto A, Sugiura T, Ohira Y, Yoshioka T, Hayashi T, Goto K.
  • Journal Title: J Caffeine Res. Volume: 6 Issue: 2 Pages: 88-96
  • DOI: 10.1089/jcr.2015.0034
  • NAID: 120006305927
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 乳酸刺激によるErk1/2の活性化が骨格筋肥大に及ぼす影響 (2017)
  • Author(s): 大野善隆，横山真吾，後藤勝正
  • Organizer: 第52回日本理学療法学術大会
• [Presentation] 筋衛星細胞の活性化に乳酸が及ぼす影響 (2017)
  • Author(s): 大野善隆，安藤孝輝，伊藤貴史，須田陽平，松井佑樹，横山真吾，江川達郎，後藤勝正
  • Organizer: 第72回日本体力医学会大会
• [Presentation] 骨格筋量の調節における乳酸受容体の役割 (2016)
  • Author(s): 大野善隆，横山真吾，江川達郎，杉浦崇夫，大平充宣，吉岡利忠，後藤勝正
  • Organizer: 第71回日本体力医学会大会
  • Place of Presentation: マリオス，アイーナ（岩手県・盛岡市）
  • Year and Date: 2016-09-23
• [Presentation] 微弱電流刺激が筋再成長に伴う細胞膜タンパク質の変化に及ぼす影響 (2016)
  • Author(s): 大野善隆，後藤勝正
  • Organizer: 第51回日本理学療法学術大会
  • Place of Presentation: 札幌コンベンションセンター，札幌市産業振興センター（北海道・札幌市）
  • Year and Date: 2016-05-27
• [Remarks] 豊橋創造大学生理学研究室ホームページ
  • URL: http://www.sozo.ac.jp/professor/goto_katsumasa/index.html