| Project/Area Number |
16K13092
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 一酸化炭素 / ヘム / シクロデキストリン / ポルフィリン / モデル錯体 / ガスバイオロジー / シグナル伝達 / 生理機能探索 |
|---|
| Outline of Final Research Achievements |
Carbon monoxide (CO) is continuously produced in mammalian cells during heme metabolic reaction. In this study, we investigated the biological function of the endogenous CO using a selective CO removal agent hemoCD. In order to introduce hemoCD into the cells, we conjugated hemoCD with octaarginine peptide. The new hemoCD derivative (R8-hemoCD) was taken by living cells and captured CO in cells, in which reactive oxygen species level was significantly enhanced in the CO-removed cells. In addition, we newly synthesized a water-soluble biomimetic model for cytochrome c oxidase to investigate the reactivity of CO with a Fe/Cu hetero-binuclear structure of CcO.
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