| Project/Area Number |
16K13093
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ケミカルバイオロジー / ライブセルイメージング / 細胞内分子送達 / 蛍光イメージング / 植物生理学 / タンパク質 / 植物 / 細胞・組織 / バイオイメージング |
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| Outline of Final Research Achievements |
In plant biology, calcium ions are involved in a variety of intriguing biological phenomena as a secondary messenger. However, most of conventional calcium indicators are not applicable for plant cells partially due to the cell wall and vacuole as the specific organelle of plant cells. Therefore, for broad application with various plants in which genetic transformation is presently infeasible, useful synthetic indicators that localize in the cytosol are highly desirable.
In this research, we developed a ligand-tethered calcium indicator suitable for applications in plant cell biology by using a natural product-based ligand of cytosolic protein. This probe showed efficient cell permeability and localized within the cytosol and nucleus by means of ligand-protein interaction, and thus enabled spatiotemporal monitoring of calcium ions in the model plant cells.
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