Selective Cell Targeting by High and Low Affinity Ligands on Cell Surface
| Project/Area Number |
16K13104
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
TANAKA Katsunori 国立研究開発法人理化学研究所, 田中生体機能合成化学研究室, 主任研究員 (00403098)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生体分子 / 糖鎖 / 細胞 / 認識 / 有機化学 |
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| Outline of Final Research Achievements |
In the field of molecular imaging, the target selectivity is one of the most important factors in determining the degree of imaging contrast. We developed an entirely unexplored idea that is based on a pre-targeting strategy by a) simultaneously recognizing two receptors using high- and low-affinity ligands and b) ligating them directly on the target cell surface. Glycan ligands with fluorescent label bound to the cell surface, even with mM binding affinity levels, can be tightly anchored by the pre-targeted high-affinity peptide ligand on the cell surface. The advantage of using low-affinity glycan ligands is that excess of the labeled glycans can be washed off from the cells. Through this approach, unspecific fluorescence background can be minimized. In this work, we demonstrated this strategy to differentiate various cancer cell lines by utilizing peptide and various different glycan structures.
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Report
(3 results)
Research Products
(56 results)
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[Journal Article] Reactivity of anti-HNK-1 antibodies to branched O-mannose glycans associated with demyelination2017
Author(s)
K. Sakuda, Y. Kizuka, Y. Yamaguchi, K. Tanaka, K. Ogiwara, T. Segawa, Y. Hagiwara, I. Matsuo, H. Ogawa, N. Taniguchi, S. Kitazume
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 487
Issue: 2
Pages: 450-456
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] In vivo gold complex catalysis within live mice2017
Author(s)
K. Tsubokura, K. K. H. Vong, A. R. Pradipta, A. Ogura, S. Urano, T. Tahara, S. Nozaki, H. Onoe, Y. Nakao, R. Sibgatullina, A. Kurbangalieva, Y. Watanabe, K. Tanaka
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Journal Title
Angew. Chem. Int. Ed.
Volume: 56
Issue: 13
Pages: 3579-3584
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Sequential double “clicks” toward structurally well-defined heterogeneous N-glycoclusters: The importance of cluster heterogeneity on pattern recognition in vivo2017
Author(s)
L. Latypova, R. Sibgatullina, A. Ogura, K. Fujiki, A. Khabibrakhmanova, T. Tahara, S. Nozaki, S. Urano, K, Tsubokura, H. Onoe, Y. Watanabe, A. Kurbangalieva, K. Tanaka
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Journal Title
Adv. Sci.
Volume: 4
Issue: 2
Pages: 1600394-1600394
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Therapeutic in vivo synthetic chemistry2017
Author(s)
Katsunori Tanaka
Organizer
International Symposium, “Systems Glycobiology and Beyond” -Toward a bridge between fundamental research and applied science-, RIKEN Wako campus, Suzuki Umetaro Hall(Wako, Saitama)
Related Report
Int'l Joint Research / Invited
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[Presentation] Theranostic Glycoconjugates2016
Author(s)
Katsunori Tanaka
Organizer
Glycoscience Japan-Netherlands Joint Seminar 2016
Place of Presentation
Leiden University Medial Center(Leiden, Netherlands)
Related Report
Int'l Joint Research / Invited
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