| Project/Area Number |
16K13116
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Brain biometrics
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Hirsae Hajime 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (90392084)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ニューロピル / GCaMP / イメージング / ニューロン・グリア / 脳 / 神経科学 |
|---|
| Outline of Final Research Achievements |
Neuropil Ca2+ dynamics has been imaged using in in vivo two-photon microscopy from the visual cortex of the G7NG817 transgenic mouse, in which astrocytes and a subpopulation of neurons express the Ca2+ sensor G-CAMP7. G7NG817 mice has been crossed with an Alzheimer’s disease model mouse line, from which similar neuropil imaging has been performed. The data were analyzed with a non-negative matrix factorization-assisted algorithm that automatically extracted individual Ca2+ active regions. Post-hoc examination of these regions showed that some e regions contained fast transients and some other regions exhibited slow transients. These are postulated to correspond to synaptopil and gliopil, respectively.
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