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Large-scale synthesis technology of pathogen detection gold nanoparticles using sugar-chain-reduction method

Research Project

Project/Area Number 16K13621
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Nanomaterials chemistry
Research InstitutionSaitama University

Principal Investigator

Koyama Tetsuo  埼玉大学, 研究機構, 技師 (20375588)

Co-Investigator(Kenkyū-buntansha) 松岡 浩司  埼玉大学, 理工学研究科, 教授 (40272281)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords金ナノ微粒子 / 病原体検査キット / 糖鎖工学 / 病原性大腸菌O157 / 糖鎖 / O157:H7 / ナノ材料 / 検査薬
Outline of Final Research Achievements

This study was to synthesize gold nanoparticles in which the sugar chain derivative is introduced on the surface. As a sugar chain derivative "Gb3" was used to confirm that gold nano particles were formed. At first, the aim of this study was the construction of fine particles that "the presence of the pathogen can be seen in a visible manner". But the color of the particles was not changed scarce than expected.
Synthesizing a polymer using the same sugar chain derivative as a raw material and dropping it into a solution together with gold nanoparticles, it became clear that the color tone change appears clearly.
Although the results were different from our initial expectations, we were able to achieve similar results.

Academic Significance and Societal Importance of the Research Achievements

本研究は『病原体の存在を色調変化で確認できる』ナノサイズの金微粒子を大量合成することを目的とした。これが実用化できれば、食品工場・学校などでも手軽に病原体の存在を確認できるはずである。
当該金微粒子は病原体が認識する『糖鎖』化合物と塩化金酸水溶液を溶液中で混合し、アルカリ条件にすることで簡便に合成できた。実際に合成した金微粒子と糖鎖を認識する分子(レクチン)を溶液中で混合した結果、色の変化は当初想定したほど明瞭で無いことが明らかとなった。しかし同じ糖を持つポリマーを金微粒子と混合したところ、目で見える色の変化が現れた。本研究で見いだした手法は病原体の検出に幅広く応用可能である。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2018 2017

All Presentation (5 results)

  • [Presentation] Synthesis of gold nanoparticles modified with sugar(III):Investigation of aggregative conditions2018

    • Author(s)
      YONAIYAMA, Tomoki; MATSUSHITA, Takahiko; KOYAMA, Tetsuo; HATANO, Ken; MATSUOKA, Koji
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シアル酸部位を含む糖修飾型金ナノ微粒子の合成 (Ⅰ): 糖鎖骨格の構築と金ナノ微粒子の作成2018

    • Author(s)
      金島崇之・松岡浩司・松下隆彦・幡野 健・小山哲夫
    • Organizer
      日本化学会第8回CSJ科学フェスタ
    • Related Report
      2018 Annual Research Report
  • [Presentation] 糖修飾型金ナノ微粒子の合成と評価(Ⅳ):糖鎖ポリマーを利用した凝集性の向上2018

    • Author(s)
      米内山友希・松下隆彦・小山哲夫・幡野 健・松岡浩司
    • Organizer
      日本化学会第8回CSJ科学フェスタ
    • Related Report
      2018 Annual Research Report
  • [Presentation] 糖鎖還元法による糖鎖含有金ナノ微粒子の実用的調製2017

    • Author(s)
      小山哲夫・米内山友希・松下隆彦・幡野健・松岡浩司
    • Organizer
      第36階 日本糖質学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 糖修飾型金ナノ微粒子の合成(Ⅰ):糖誘導体を用いた金の還元2017

    • Author(s)
      米内山友希・松下隆彦・小山哲夫・幡野健・松岡浩司
    • Organizer
      日本化学会秋季事業 第7回 CSJ化学フェスタ2017
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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