Design of peptide probes capable of recognizing highly-curved membranes for exosome analysis

Research Project

Project/Area Number	16K14013
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Analytical chemistry
Research Institution	Tohoku University
Principal Investigator	Sato yusuke 東北大学, 理学研究科, 助教 (90583039)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	蛍光プローブ / 両親媒性αヘリックス / エクソソーム / 高曲率性 / イメージング / エキソソーム / ペプチド / 高曲率 / 蛍光性プローブ
Outline of Final Research Achievements	We developed fluorescent probes based on amphipathic αhelix peptide (22 residues from C-terminal of ApoA-I) that can selectively recognize highly-curved membranes found in exosomes. Our method allows easier, simpler and more rapid detection of exosomes.