A time-resolved analysis of intracellular signal transduction in response to non-diffusible factor

• Project/Area Number: 16K14026
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: National Institute for Materials Science
• Principal Investigator: Nakanishi Jun 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, グループリーダー (60360608)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ナノバイオ / シグナル伝達 / 細胞・組織 / 生体材料 / ケージド化合物

Outline of Final Research Achievements

Some diffusible biomolecules change their activities upon their conjugation to materials. In this project, we developed new research tools for cellular responses to material-biomolecule conjugates. We have succeeded in observation dynamic processes of estradiol or epidermal growth factor conjugated to micropatterned substrates and nanoparticles, respectively. These methods will give us useful information for the developments of new biomaterials and drugs.

Research Products

• [Int'l Joint Research] Univ. Montreal(カナダ)
• [Journal Article] Estradiol-tethered micropatterned surfaces for the study of estrogenic non-genomic pathways (2016)
  • Author(s): B. Qi, Y. Shimizu, J. Nakanishi*, and F. M. Winnik
  • Journal Title: Chemical Communications Volume: 52 Issue: 65 Pages: 10056-10059
  • DOI: 10.1039/c6cc03899a
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 金ナノ粒子固定化上皮成長因子の 特異的アポトーシス誘導活性に対する脂質ラフトの役割 (2018)
  • Author(s): 山本翔太、岩丸祥史、清水善久、山口和夫、中西淳
  • Organizer: つくば医工連携フォーラム2018
• [Presentation] A mechanism study of unique apoptosis-inducing activity of epidermal growth factor immobilized on gold nanoparticles (2018)
  • Author(s): S. Yamamoto, Y. Iwamaru, Y. Shimizu, K. Yamaguchi, J. Nakanishi
  • Organizer: MANA Symposium 2018
  • Int'l Joint Research
• [Presentation] 上皮成長因子担持金ナノ粒子が特異的に誘起するアポトーシス活性に対する脂質ラフトの関与 (2017)
  • Author(s): 山本翔太、岩丸祥史、清水善久、山口和夫、中西淳
  • Organizer: 分析化学第66年会
• [Presentation] 上皮成長因子担持金ナノ粒子の特異的アポトーシス誘導活性における脂質ラフトの役割 (2017)
  • Author(s): 山本翔太、岩丸祥史、清水善久、山口和夫、中西淳
  • Organizer: 第39回バイオマテリアル学会大会
• [Presentation] Role of lipid rafts in unique apoptosis inducing activity of epidermal growth factor-gold nanoparticles conjugates (2017)
  • Author(s): S. Yamamoto, Y. Iwamaru, Y. Shimizu, K. Yamaguchi, J. Nakanishi
  • Organizer: 第27回日本MRS
  • Int'l Joint Research
• [Presentation] 上皮成長因子-金ナノ粒子コンジュゲートの特異なアポトーシス誘導活性のメカニズム探究 (2017)
  • Author(s): 中西淳
  • Organizer: RIKEN-iCONM-NIMS医工学ネットワーク
  • Invited