Development of two-dimensional DNA origami structure for construction of molecular delivery system
Project/Area Number |
16K14033
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
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Research Institution | Kyoto University |
Principal Investigator |
Endo Masayuki 京都大学, 理学研究科, 特定准教授 (70335389)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | DNAナノテクノロジー / DNAオリガミ / リポソーム / 脂質膜 / 原子間力顕微鏡 / デリバリーシステム / 細胞機能制御 / DNAナノ構造体 / 人工受容体 / 細胞 / ドラッグデリバリー |
Outline of Final Research Achievements |
In this study, we aimed to develop new strategy by constructing DNA nanostructures which can penetrate the cell membrane and application to molecular delivery system. We designed and constructed a DNA origami structure having a needle-like portion that can penetrate the lipid membrane, and observed that it interacts with the lipid bilayer membrane and forms a lattice assembly by high-speed AFM. By using liposome which is a model system of the cell, the target molecule was successfully introduced into the liposome and biochemical reaction was initiated by penetrating the lipid membrane. Using this system, we develop a new system that can introduce the target molecule from the cell surface into the cell.
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Report
(3 results)
Research Products
(49 results)
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[Journal Article] Holliday junction resolvases mediate chloroplast nucleoid segregation2017
Author(s)
Kobayashi Y, Misumi O, Odahara M, Ishibashi K, Hirono M, Hidaka K, Endo M, Sugiyama H, Iwasaki H, Kuroiwa T, Shikanai T, Nishimura Y.
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Journal Title
Science
Volume: 356
Issue: 6338
Pages: 631-634
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate.2017
Author(s)
Tomonori Shibata, Yoshihiko Fujita, Hirohisa Ohno, Yuki Suzuki, Karin Hayashi, Kaoru R. Komatsu, Shunsuke Kawasaki, Kumi Hidaka, Shin Yonehara, Hiroshi Sugiyama, Masayuki Endo and Hirohide Saito
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Journal Title
Nature Communications
Volume: 8
Issue: 1
Pages: 540-540
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Self-assembling DNA hydrogel-based delivery of immunoinhibitory nucleic acids to immune cells.2016
Author(s)
Nishida Y, Ohtsuki S, Araie Y, Umeki Y, Endo M, Emura T, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y, Nishikawa M.
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Journal Title
Nanomedicine: Nanotechnology, Biology and Medicine
Volume: 12
Issue: 1
Pages: 123-130
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] DNA鎖の拘束によるCas9切断への影響2017
Author(s)
Michael H. Reaz, 日高 久美, Shana J. Sturla, 杉山 弘, 遠藤 政幸
Organizer
日本化学会第97春季年会(2017)
Place of Presentation
慶応大学日吉キャンパス
Year and Date
2017-03-18
Related Report
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