2017 Fiscal Year Final Research Report
Development of two-dimensional DNA origami structure for construction of molecular delivery system
Project/Area Number |
16K14033
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
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Research Institution | Kyoto University |
Principal Investigator |
Endo Masayuki 京都大学, 理学研究科, 特定准教授 (70335389)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Keywords | DNAナノテクノロジー / DNAオリガミ / リポソーム / 脂質膜 / 原子間力顕微鏡 / デリバリーシステム / 細胞機能制御 |
Outline of Final Research Achievements |
In this study, we aimed to develop new strategy by constructing DNA nanostructures which can penetrate the cell membrane and application to molecular delivery system. We designed and constructed a DNA origami structure having a needle-like portion that can penetrate the lipid membrane, and observed that it interacts with the lipid bilayer membrane and forms a lattice assembly by high-speed AFM. By using liposome which is a model system of the cell, the target molecule was successfully introduced into the liposome and biochemical reaction was initiated by penetrating the lipid membrane. Using this system, we develop a new system that can introduce the target molecule from the cell surface into the cell.
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Free Research Field |
生物有機化学
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