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Construction of Artificial Light Harvesting System Using Hemoprotein Assemblies

Research Project

Project/Area Number 16K14036
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Research InstitutionOsaka University

Principal Investigator

Hayashi Takashi  大阪大学, 工学研究科, 教授 (20222226)

Research Collaborator Oohora Koji  大阪大学, 大学院工学研究科, 助教 (10631202)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords人工光合成 / 光捕集 / ヘムタンパク質 / 触媒 / 補因子 / 色素
Outline of Final Research Achievements

This study has focused on generation of artificial photosynthesis by the combination of protein-based artificial light harvesting systems and catalysts. To achieve this goal, we especially demonstrated the construction of the new-types of hemoprotein assemblies and an artificial light harvesting system. In the former, micelle-like, heroical and ring-shaped assemblies were prepared and characterized as pigment-containing protein assemblies as scaffolds of light harvesting systems. In the latter, multiple photosensitizers were introduced to hexameric hemoprotein and its light harvesting function was evaluated. Furthermore, modification of the artificial light harvesting system by synthesized catalysts was attempted for the photo-induced catalysis.

Academic Significance and Societal Importance of the Research Achievements

本研究では、人工合成を最終ゴールとして、新規なタンパク質を基盤とする光捕集系と触媒の導入を研究した。近年のエネルギー問題を考える上で、人工合成の実現は非常に重要な課題であり、天然光合成系でも利用されているタンパク質は光捕集系の足場として、有用であることが本研究の学術的知見として得られた。今後、導入する触媒の検討や最適化により太陽光の効率的な利用によるエネルギー生産や物質変換への応用が期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (27 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 12 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 6 results,  Invited: 6 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] Technological University of Eindhoven(オランダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Technological University of Eindhoven(オランダ)

    • Related Report
      2017 Research-status Report
  • [Journal Article] A ring-shaped hemoprotein trimer thermodynamically controlled by the supramolecular heme-heme pocket interaction2019

    • Author(s)
      Oohora Koji、Kajihara Ryota、Fujimaki Nishiki、Uchihashi Takayuki、Hayashi Takashi
    • Journal Title

      Chemical Communications

      Volume: 55 Issue: 11 Pages: 1544-1547

    • DOI

      10.1039/c8cc09314h

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced electron transfer within supramolecular hemoprotein co-assemblies and heterodimers containing Fe and Zn porphyrins2019

    • Author(s)
      Kajihara Ryota、Oohora Koji、Hayashi Takashi
    • Journal Title

      Journal of Inorganic Biochemistry

      Volume: 193 Pages: 42-51

    • DOI

      10.1016/j.jinorgbio.2019.01.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arginine Residues Provide a Multivalent Effect for Cellular Uptake of a Hemoprotein Assembly2019

    • Author(s)
      Oohora Koji、Kajihara Ryota、Jiromaru Misa、Kitagishi Hiroaki、Hayashi Takashi
    • Journal Title

      Chemistry Letters

      Volume: 48 Issue: 3 Pages: 295-298

    • DOI

      10.1246/cl.180897

    • NAID

      130007607775

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Supramolecular Hemoprotein Assembly with a Periodic Structure Showing Heme-Heme Exciton Coupling2018

    • Author(s)
      Oohora Koji、Fujimaki Nishiki、Kajihara Ryota、Watanabe Hiroki、Uchihashi Takayuki、Hayashi Takashi
    • Journal Title

      Journal of the American Chemical Society

      Volume: 140 Issue: 32 Pages: 10145-10148

    • DOI

      10.1021/jacs.8b06690

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successive energy transfer within multiple photosensitizers assembled in a hexameric hemoprotein scaffold2018

    • Author(s)
      Mashima Tsuyoshi、Oohora Koji、Hayashi Takashi
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 20 Issue: 5 Pages: 3200-3209

    • DOI

      10.1039/c7cp05257j

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] A supramolecular assembly based on an engineered hemoprotein exhibiting a thermal stimulus-driven conversion to a new distinct supramolecular structure2017

    • Author(s)
      Oohora Koji、Onuma Yoshitaka、Tanaka Yuta、Onoda Akira、Hayashi Takashi
    • Journal Title

      Chemical Communications

      Volume: 53 Issue: 51 Pages: 6879-6882

    • DOI

      10.1039/c7cc02678a

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Intraprotein transmethylation via a CH3–Co(III) species in myoglobin reconstituted with a cobalt corrinoid complex2016

    • Author(s)
      Y. Morita, K. Oohora, A. Sawada, K. Doitomi, J. Ohbayashi, T. Kamachi, K. Yoshizawa, Y. Hisaeda, T. Hayashi
    • Journal Title

      Dalton Transactions

      Volume: 45 Issue: 8 Pages: 3277-3284

    • DOI

      10.1039/c5dt04109k

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Construction of a hybrid biocatalyst containing a covalently-linked terpyridine metal complex within a cavity of aponitrobindin2016

    • Author(s)
      Tomoki Himiyama, Daniel F. Sauer, Akira Onoda, Thomas P. Spaniol, Jun Okuda*, and Takashi Hayashi
    • Journal Title

      J. Inorg. Biochem.

      Volume: in press Pages: 55-61

    • DOI

      10.1016/j.jinorgbio.2015.12.026

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Crystal Structures and Coordination Behavior of Aqua- and Cyano-Co(III) Tetradehydrocorrins in the Heme Pocket of Myoglobin2016

    • Author(s)
      Y. Morita, K. Oohora, E. Mizohata, A. Sawada, T. Kamachi, K. Yoshizawa, T. Inoue, T. Hayashi
    • Journal Title

      Inorganic Chemistry

      Volume: 55 Issue: 3 Pages: 1287-1295

    • DOI

      10.1021/acs.inorgchem.5b02598

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Oxygen-binding Protein Fiber and Microgel: Supramolecular Myoglobin–Poly(acrylate) Conjugates2016

    • Author(s)
      T. Ono, Y. Hisaoka, A. Onoda, K. Oohora, T. Hayashi
    • Journal Title

      Chem. Asian J.

      Volume: 41 Issue: 7 Pages: 1036-1042

    • DOI

      10.1002/asia.201501415

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Artificial Diels-Alderase Based on the Transmembrane Protein FhuA2016

    • Author(s)
      H. Osseili, D. F. Sauer, K. Beckerle, M. Arlt, T. Himiyama, T. Polen, A. Onoda, U. Schwaneberg, T. Hayashi, J. Okuda*
    • Journal Title

      Beilstein J. Org. Chem.

      Volume: 12 Pages: 1314-1321

    • DOI

      10.3762/bjoc.12.124

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Anchoring Cytochrome b562 on a Gold Nanoparticle by a Heme-Heme Pocket Interaction2016

    • Author(s)
      A. Onoda,* T. Taniguchi, N. Inoue, A. Kamii, T. Hayashi*
    • Journal Title

      Eur. J. Inorg. Chem.

      Volume: 2016 Issue: 21 Pages: 3454-3459

    • DOI

      10.1002/ejic.201600301

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Supramolecular Assembly of Engineered Cytochrome b562 Forming a Thermodynamically Stable Ring-shaped Trimer2019

    • Author(s)
      Koji Oohora, Ryota Kajihara, Takashi Hayashi
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Construction of a Micellar Assembly of Hexameric Hemoprotein and Evaluation of its Photochemical Behavior2019

    • Author(s)
      Shota Hirayama, Koji Oohora, Takashi Hayashi
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞内導入を指向したヘムタンパク質超分子集合体の構築2018

    • Author(s)
      大洞 光司, 梶原 竜太, 林 高史
    • Organizer
      第16回ホスト-ゲスト・超分子化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 機能性分子を化学修飾した六量体ヘムタンパク質の自己集合化2018

    • Author(s)
      平山 翔太, 大洞 光司, 林 高史
    • Organizer
      第7回 JACI/GSCシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 六量体ヘムタンパク質への化学修飾によるミセル様タンパク質集合体の構築およびその光化学的評価2018

    • Author(s)
      平山 翔太, 大洞 光司, 林 高史
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Supramolecular Hemoprotein Assemblies2018

    • Author(s)
      Takashi Hayashi
    • Organizer
      Bionano2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Supramolecular Hemoprotein Assembly toward Photoinduced Catalysts2017

    • Author(s)
      Takashi Hayashi
    • Organizer
      1st Molecular Technology Workshop: “Energy and Electron Transfers in Molecular Engineered Materials”
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] A Biohybrid Catalyst Using a Heme-Pocket with an Artificial Metallocomplex2017

    • Author(s)
      Takashi Hayashi
    • Organizer
      6th Asian Conference of Coordination Chemistry (ACCC6)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supramolecular Hemoprotein Assemblies via Heme-Heme Pocket Interaction2017

    • Author(s)
      Takashi Hayashi
    • Organizer
      11th Japan-China Joint Symposium on Metal Cluster Compounds
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Preparation, Characterization and Photochemical Properties of Supramolecular Hemoprotein Assemblies2016

    • Author(s)
      Takashi Hayashi, Koji Oohora
    • Organizer
      BIONANO2016
    • Place of Presentation
      Krakow, Poland
    • Year and Date
      2016-11-23
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Construction of Hemoprotein Assembly via Heme-heme Pocket Interaction and its Photochemical Property2016

    • Author(s)
      Takaashi Hayashi, Tsuyoshi Mashima, Shota Hirayama, Koji Oohora
    • Organizer
      229th ECS Meeting
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-06-02
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] Artificial Hemoprotein Assemblies in Development of Nanobiomaterials in Bioinspired Chemistry From Enzymes to Synthetic Models2019

    • Author(s)
      Koji Oohora, Takashi Hayashi
    • Total Pages
      18
    • Publisher
      World Scientific
    • ISBN
      9789813274433
    • Related Report
      2018 Annual Research Report
  • [Remarks] 大阪大学大学院工学研究科林研究室HP

    • URL

      http://www.chem.eng.osaka-u.ac.jp/~hayashiken/index.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-02-22  

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