Solar-powered bioremediation by bacterial transporters
Project/Area Number |
16K14044
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Green/Environmental chemistry
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Research Institution | Hokkaido University |
Principal Investigator |
Demura Makoto 北海道大学, 先端生命科学研究院, 教授 (70188704)
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Research Collaborator |
KIKUKAWA takashi
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | イオンポンプ / プロトンポンプ / ナトリウムポンプ / 多剤排出 / 微生物ロドプシン / エネルギー共役 / 環境技術 / 生物物理 / 応用微生物 / 蛋白質 |
Outline of Final Research Achievements |
We aimed to make an energy-coupling system between light-driven ion-pumping microbial rhodopsins and ion-coupled multidrug transporters for future development of a solar-powered bioremediation by bacterial cells. The results could be summarized as follows: 1) Compared to H+ pump rhodopsin, Na+ pump rhodopsin was revealed to efficiently drive the drug transporter, 2) From a Gram-positive bacterium inhabiting Antarctica, we found a strong inward H+ pump rhodopsin, which might be utilized to drive the inward multidrug transport.
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Academic Significance and Societal Importance of the Research Achievements |
細菌の細胞膜には種々の物質輸送蛋白質が存在する。本研究では、微生物型ロドプシンと多剤排出蛋白質の2種の蛋白質に注目した。微生物型ロドプシンは、光をエネルギー源として細胞膜を隔てたH+やNa+の濃度勾配を作り出すことができる。一方、多剤排出蛋白質は、これらのイオン濃度勾配を利用して、様々な薬物を膜輸送できる。これらを組み合わせることで、太陽光をエネルギー源として、環境中の様々な薬物を細胞内へ蓄積する「環境浄化細菌」を作製できる可能性がある。この作製のために、1)薬物輸送の効率を高める方法の検討、2)既存の微生物型ロドプシンの機能強化、3)高い機能を持つ微生物型ロドプシンの探索を行った。
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Photochemical characterization of actinorhodopsin and its functional existence in the natural host2016
Author(s)
Nakamura, S., Kikukawa, T., Tamogami, J., Kamiya, M., Aizawa, T., Hahn, M., Ihara, K., Kamo, N., Demura, M.
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Journal Title
BBA-BIOENERGETICS
Volume: 1857
Issue: 12
Pages: 1900-1908
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Functional analyses of Na+-pumping rhodopsin focusing on acidic residues on the extracellular surface2017
Author(s)
Okamura, A., Kikukawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Demura, M.
Organizer
第55回日本生物物理学会年会
Related Report
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[Presentation] Analysis of Na+ transfer reactions of Na+-pumping rhodopsin2017
Author(s)
Murabe, K., Kikukawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Demura, M.
Organizer
第55回日本生物物理学会年会
Related Report
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[Presentation] Functional importance of trimer formation of light-driven H+ pump Gloeobacter rhodopsin2017
Author(s)
Iizuka, A., Kikukawa, T., Kajimoto, K., Fujisawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Unno, M., Demura, M.
Organizer
第55回日本生物物理学会年会
Related Report
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[Presentation] Functional analysis of T126E mutant of Natronomonas pharaonis halorhodopsin2017
Author(s)
Abe, S., Kikukawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Demura, M.
Organizer
第55回日本生物物理学会年会
Related Report
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[Presentation] Solid-state NMR analysis of sodium ion pump rhodopsin and its Na+-binding2016
Author(s)
Tamaki, H., Saito, Y., Egawa, A., Kikukawa, T., Fujiwara, T., Demura, M.
Organizer
17th International Conference on Retinal Proteins
Place of Presentation
ポツダム(ドイツ)
Year and Date
2016-10-03
Related Report
Int'l Joint Research
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[Presentation] Replacements of “donor” residues in the light- driven H+-pump rhodopsins2016
Author(s)
Nishiya, K., Sasaki, S., Tamogami, J., Kikukawa, T., Aizawa, T., Kamo, N., Demura, M.
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県・筑波)
Related Report
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