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Estimation with high spatio-temporal resolution on abundance of stream fish using environmental DNA analysis

Research Project

Project/Area Number 16K14313
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Hydraulic engineering
Research InstitutionYamaguchi University

Principal Investigator

Akamatsu Yoshihisa  山口大学, 大学院創成科学研究科, 准教授 (30448584)

Co-Investigator(Kenkyū-buntansha) 高原 輝彦  島根大学, 生物資源科学部, 助教 (10536048)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords環境DNA / アユ / カワムツ / バイオマス / 生物量推定 / 生物量 / 佐波川 / 水工水理学 / 環境質定量化・予測 / 環境分析
Outline of Final Research Achievements

In this study, we conducted three experiments using fish and simulation of dynamics of eDNA. They showed that (1) the amount of released eDNA were higher in slow velocity than fast velocity and eDNA concentration and biomass have a strong positive correlation in a running water, (2) the influence distance of eDNA in stream was between 1000m and 2000m, (3) excrement settles faster than mucus in static water and (4) the flow model showed that the distance of all particles containing eDNA settling was shorter than real stream.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2017

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] 流水中におけるカワムツの生物量と環境DNA量の関係性-水路実験と野外への適用-2017

    • Author(s)
      乾 隆帝,赤松良久,高原輝彦,後藤益滋,一松晃弘
    • Journal Title

      河川技術論文集

      Volume: 23 Pages: 651-656

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 江の川における環境 DNA 分析を用いたアユの定量化と生物量に影響を与える環境要因の検討2017

    • Author(s)
      乾隆帝,後藤益滋,河野誉仁,赤松良久,掛波優作,一松晃弘
    • Journal Title

      土木学会論文集B1(水工学)

      Volume: 73 Pages: 1105-1110

    • NAID

      130006406106

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 環境DNAを用いた河川内の魚類現存量推定に関する基礎的検討2017

    • Author(s)
      赤松良久,乾 隆帝,一松晃弘,河野誉仁,土居秀幸
    • Journal Title

      土木学会論文集B1(水工学)

      Volume: 73 Pages: 1111-1116

    • NAID

      130006406105

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Presentation] 流水中におけるカワムツの生物量と環境DNA量の関係性-水路実験と野外への適用-2017

    • Author(s)
      乾 隆帝
    • Organizer
      河川技術シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 江の川における環境 DNA 分析を用いたアユの定量化と生物量に影響を与える環境要因の検討2017

    • Author(s)
      乾隆帝
    • Organizer
      水工学講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 環境DNAを用いた河川内の魚類現存量推定に関する基礎的検討2017

    • Author(s)
      赤松良久
    • Organizer
      水工学講演会
    • Related Report
      2017 Annual Research Report 2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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