Devising a novel light-regulated channel based on minimum unit of voltage sensor domain
Project/Area Number |
16K14555
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Osaka University |
Principal Investigator |
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Research Collaborator |
Fujiwara Yuichiro
Kawai Takafumi
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | イオンチャネル / 光遺伝学 / 電位センサー / 膜電位 / LOVドメイン / 電位依存性プロトンチャネル / 精子特異的カチオンチャネル / カルシウムイオン / 生理学 / 脳・神経 / 蛋白質 / シグナル伝達 / 酵素 |
Outline of Final Research Achievements |
This project aims at devising a novel light-regulated ion channel based on the minimum ion channel unit derived from voltage-gated proton channel, VSOP/Hv1. LOV domain changes its structure upon reception of light by flavin molecule. Light-sensitivity will be conferred to the minimum unit of ion channel of Hv1 and other VSD-derived polypeptide that have ion channel activities by fusion with LOV domain. In the long term, establishment of platform for developing useful molecular tools for neuroscience will be constructed.
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Academic Significance and Societal Importance of the Research Achievements |
近年の遺伝学的技術の進歩により、従来破壊実験などに頼ってきた実験医学の手法が革新され、生体内の特定の細胞機能を一過的に攪乱させ解析することが可能になった。そうした技術の中でも光遺伝学は神経科学において常套手段に至ったものの、分子ツールは自然界に存在する光感受性分子そのもの、または微調整した分子に限定されてきた。本研究はイオンチャネルの仕組みが詳細に理解されているHv1に着目し、動作原理に基づいて光感受性タンパク質との融合分子を構築することで柔軟に分子ツールを構築するプラットフォームを目指す。本研究で期待される成果は脳の神経回路の動作原理解明や治療に用いる代替え組織の創製技術などに資する。
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Report
(4 results)
Research Products
(103 results)
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[Journal Article] Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging.2017
Author(s)
Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels M, Okamura Y, Matsuda M, and Nagai T
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 42398-42398
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] A new mechanism of Ca2+-independent voltage-dependent secretion in dorsal root ganglion neurons2019
Author(s)
Zhuan Zhou, Yuan Wang, Hiroki Arima, Rong Huang, Yuqi Hang, Xingyu Du, Feipeng Zhu, Zuying Chai, Changhe Wang, Yasushi Okamura
Organizer
The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
Related Report
Int'l Joint Research
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[Presentation] Functional analysis of voltage-sensing phosphatase in mouse sperm2019
Author(s)
Takafumi Kawai, Haruhiko Miyata, Hiroki Nakanishi, Souhei Sakata, Yoshifumi Okochi, Masahiko Watanabe, Kenji Sakimura, Takehiko Sasaki, Masahito Ikawa, Yasushi Okamura
Organizer
The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
Related Report
Int'l Joint Research
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[Presentation] Development of a chemiluminescent voltage indicator applicable to brain activity recording in freely moving multiple mice2017
Author(s)
Shigenori Inagaki, Masakazu Agetsuma, Hidekazu Tsutsui, Shinya Ohara, Yoshiyuki Arai, Kazushi Suzuki, Yuka Jinno, Tomoki Matsuda, Toshio Iijima, Yasushi Okamura, Takeharu Nagai
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] Molecular dynamics study of kinetics of the voltage-gated proton channel VSOP/Hv1 under electric fields2017
Author(s)
Hiroko X. Kondo, Yasushige Yonezawa, Naoyuki Miyashita, Masayo Iwaki,Kohei Takeshita, Yuichiro Fujiwara, Matsuyuki Shirota, Kengo Kinoshita, Yasushi Okamura, Atsushi Nakagawa, Hideki Kandori, Yu Takano
Organizer
第54回日本生物物理学会年会
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Invited
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[Book] ガイトン生理学 原著第13版2018
Author(s)
石川義弘、岡村康司(総監訳)、尾仲達史、河野憲二
Total Pages
1100
Publisher
エルゼビア・ジャパン株式会社
ISBN
9784860347741
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