A feasibility study for 3D neuropathology
Project/Area Number |
16K14571
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Nerve anatomy/Neuropathology
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Research Institution | Niigata University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
北浦 弘樹 新潟大学, 脳研究所, 助教 (80401769)
清水 宏 新潟大学, 脳研究所, 助教 (40608767)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 神経細胞病理学 / 脳透明化技術 / てんかん / 神経病理学 / 透明化 / 3次元解析 / 神経細胞配列 |
Outline of Final Research Achievements |
To investigate the 3-dimensional cytoarchitecture of the cerebral cortex taken from patients with epilepsy, we developed new chemical protocols for clearing human brain tissue and driving methodology of the sheet-scanning fluorescence microscopy. By the methods, we were able to observe fine features of neurons and glia distributed widely within extremely thick brain blocks. The methods created by this feasibility study would open an innovative era for the neuropathology field.
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Report
(3 results)
Research Products
(36 results)
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[Journal Article] Biallelic variants in CNPY3, which encodes an endoplasmic reticulum chaperone, cause early-onset epileptic encephalaopathy.2018
Author(s)
Mutoh H, Kato M, Akita T, Shibata T, Wakamoto H, Ikeda H, Kitaura H, Aoto K, Nakashima M, Wang T, Ohba C, Miyatake S, Miyake N, Kakita A, Miyake K, Fukuda A, Matsumoto N, Saitsu H
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Journal Title
Am J Hum Genet
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Intractable epilepsy due to a rosette-forming glioneuronal tumor with a dysembryoplastic neuroepithelial background.2017
Author(s)
Sumitomo N, Ishiyama A, Shibuya M, Nakagawa E, Kaneko Y, Takahashi A, Otsuki T, Kakita A, Saito Y, Sato N, Sugai K, Sasaki M.
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Journal Title
Neuropathology
Volume: 12
Issue: 3
Pages: 300-304
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Comprehensive genetic characterizeation of rosette-forming glioneuronal tumors: independent component analysis by tissue microdissection.2017
Author(s)
Kitamura Y, Komori T, Shibuya M, Ohara K, Saito Y, Hayashi S, Sasaki A, Nakagawa E, Tomio R, Kakita A, Nakatsukasa M, Yoshida K, Sasaki H.
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Journal Title
Brain Pathology
Volume: 印刷中
Issue: 1
Pages: 87-93
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Ca2+-permiable AMPA receptors associated with epileptogenesis of hypothalamic hamartoma.2017
Author(s)
Kitaura H, Sonoda M, Teramoto S, Shirozu H, Shimizu H, Kimura M, Masuda H, Ito Y, Takahashi H, Kwak S, Kameyama S, Kakita A.
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Journal Title
Epilepsia
Volume: 58
Issue: 4
Pages: 59-63
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] MRI病変の指摘がないが機能画像等により焦点切除術を行った小児てんかん患者の臨床的特徴.2018
Author(s)
斎藤良彦, 須貝研司, 竹下絵里, 本橋裕子, 石山昭彦, 齋藤貴志, 小牧宏文, 中川栄二, 佐藤典子, 柿田明美, 齊藤祐子, 大槻泰介, 岩崎真樹, 佐々木征行.
Organizer
第60回 小児神経学会
Related Report
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[Presentation] Biallelic variants in CNPY3 cause West syndrome with hippocampal malrotation and characteristic fast waves.2018
Author(s)
Kato M, Mutoh H, Akita T, Shibata T, Wakamoto H, Ikeda H, Kitaura H, Aoto K, Nakashima M, Wang T, Ohba C, Miyatake S, Miyake N, Kakita A, Miyake K, Fukuda A, Matsumoto N, Saitsu H.
Organizer
13th European Congress on Epileptology
Related Report
Int'l Joint Research
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