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A feasibility study for 3D neuropathology

Research Project

Project/Area Number 16K14571
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Nerve anatomy/Neuropathology
Research InstitutionNiigata University

Principal Investigator

Kakita Akiyoshi  新潟大学, 脳研究所, 教授 (80281012)

Co-Investigator(Kenkyū-buntansha) 北浦 弘樹  新潟大学, 脳研究所, 助教 (80401769)
清水 宏  新潟大学, 脳研究所, 助教 (40608767)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords神経細胞病理学 / 脳透明化技術 / てんかん / 神経病理学 / 透明化 / 3次元解析 / 神経細胞配列
Outline of Final Research Achievements

To investigate the 3-dimensional cytoarchitecture of the cerebral cortex taken from patients with epilepsy, we developed new chemical protocols for clearing human brain tissue and driving methodology of the sheet-scanning fluorescence microscopy. By the methods, we were able to observe fine features of neurons and glia distributed widely within extremely thick brain blocks. The methods created by this feasibility study would open an innovative era for the neuropathology field.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (36 results)

All 2018 2017 2016

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (25 results) (of which Int'l Joint Research: 8 results,  Invited: 10 results) Book (2 results)

  • [Journal Article] フラビン蛍光イメージングによるてんかん原性の解析.2018

    • Author(s)
      北浦弘樹、柿田明美.
    • Journal Title

      Clin Neurosci

      Volume: 36

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Biallelic variants in CNPY3, which encodes an endoplasmic reticulum chaperone, cause early-onset epileptic encephalaopathy.2018

    • Author(s)
      Mutoh H, Kato M, Akita T, Shibata T, Wakamoto H, Ikeda H, Kitaura H, Aoto K, Nakashima M, Wang T, Ohba C, Miyatake S, Miyake N, Kakita A, Miyake K, Fukuda A, Matsumoto N, Saitsu H
    • Journal Title

      Am J Hum Genet

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expansions of intronic TTTCA and TTTTA repeats in benign adult familial myoclonic epilepsy2018

    • Author(s)
      Ishiura Hiroyuki、Doi Koichiro、Mitsui Jun、Yoshimura Jun、Matsukawa Miho Kawabe、Takahashi Yuji、Date Hidetoshi、Matsukawa Takashi、Shimizu Jun、Koh Kishin、Takiyama Yoshihisa、Goto Jun、Morishita Shinichi、Tsuji Shoji
    • Journal Title

      Nature Genetics

      Volume: 50 Issue: 4 Pages: 581-590

    • DOI

      10.1038/s41588-018-0067-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pathophysiological characteristics associated with epileptogenesis in Human Hippocampal sclerosis.2018

    • Author(s)
      Kitaura H, Shirozu H, Masuda H, Fukuda M, Fujii Y, Kakita A
    • Journal Title

      EBioMedicine

      Volume: 29 Pages: 38-46

    • DOI

      10.1016/j.ebiom.2018.02.013

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intractable epilepsy due to a rosette-forming glioneuronal tumor with a dysembryoplastic neuroepithelial background.2017

    • Author(s)
      Sumitomo N, Ishiyama A, Shibuya M, Nakagawa E, Kaneko Y, Takahashi A, Otsuki T, Kakita A, Saito Y, Sato N, Sugai K, Sasaki M.
    • Journal Title

      Neuropathology

      Volume: 12 Issue: 3 Pages: 300-304

    • DOI

      10.1111/neup.12450

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comprehensive genetic characterizeation of rosette-forming glioneuronal tumors: independent component analysis by tissue microdissection.2017

    • Author(s)
      Kitamura Y, Komori T, Shibuya M, Ohara K, Saito Y, Hayashi S, Sasaki A, Nakagawa E, Tomio R, Kakita A, Nakatsukasa M, Yoshida K, Sasaki H.
    • Journal Title

      Brain Pathology

      Volume: 印刷中 Issue: 1 Pages: 87-93

    • DOI

      10.1111/bpa.12468

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Features of amygdala in patients with mesial temporal lobe epilepsy and hippocampal sclerosis: An MRI volumetric and histopathological study.2017

    • Author(s)
      Nakayama Y, Masuda H, Shirozu H, Ito Y, Higashijima T, Kitaura H, Fujii Y, Kakita A, Fukuda M.
    • Journal Title

      Epilepsy Res

      Volume: 135 Pages: 50-55

    • DOI

      10.1016/j.eplepsyres.2017.05.010

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ca2+-permiable AMPA receptors associated with epileptogenesis of hypothalamic hamartoma.2017

    • Author(s)
      Kitaura H, Sonoda M, Teramoto S, Shirozu H, Shimizu H, Kimura M, Masuda H, Ito Y, Takahashi H, Kwak S, Kameyama S, Kakita A.
    • Journal Title

      Epilepsia

      Volume: 58 Issue: 4 Pages: 59-63

    • DOI

      10.1111/epi.13700

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] mTORとてんかん.2016

    • Author(s)
      北浦弘樹、武井延之、中島光子、松本直通、柿田明美.
    • Journal Title

      Epilepsy

      Volume: 10 Pages: 97-102

    • Related Report
      2016 Research-status Report
  • [Presentation] MRI病変の指摘がないが機能画像等により焦点切除術を行った小児てんかん患者の臨床的特徴.2018

    • Author(s)
      斎藤良彦, 須貝研司, 竹下絵里, 本橋裕子, 石山昭彦, 齋藤貴志, 小牧宏文, 中川栄二, 佐藤典子, 柿田明美, 齊藤祐子, 大槻泰介, 岩崎真樹, 佐々木征行.
    • Organizer
      第60回 小児神経学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 限局性皮質異形成でT1強調高信号を呈した症例の検討.2018

    • Author(s)
      老谷嘉樹、木村有喜男、須貝研司、齊藤祐子、池谷直樹、岩崎 真樹、竹下絵里、本橋裕子、石山昭彦、齋藤貴志、小牧宏文、中川栄二、柿田明美、佐藤典子、佐々木征行.
    • Organizer
      第60回 小児神経学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Biallelic variants in CNPY3 cause West syndrome with hippocampal malrotation and characteristic fast waves.2018

    • Author(s)
      Kato M, Mutoh H, Akita T, Shibata T, Wakamoto H, Ikeda H, Kitaura H, Aoto K, Nakashima M, Wang T, Ohba C, Miyatake S, Miyake N, Kakita A, Miyake K, Fukuda A, Matsumoto N, Saitsu H.
    • Organizer
      13th European Congress on Epileptology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of 3D neuropathology based on tissue clearing technique.2018

    • Author(s)
      Tainaka K, Saito R, Kakita A.
    • Organizer
      International Congress of Neuropathology 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comprehensive analysis of protein expression profiles in sclerotic hippocampus from patients with mesial temporal lobe epilepsy.2018

    • Author(s)
      Furukawa A, Kakita A, Chiba Y, Kameyama S, Shimada A.
    • Organizer
      International Congress of Neuropathology 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Epileptogenesis of the subiculum associated with hippocampal sclerosis in patients with MTLE.2018

    • Author(s)
      Kitaura H, Shiroku H, Masuda H, Fukuda M, Fujii Y, Kakita A.
    • Organizer
      International Congress of Neuropathology 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] てんかん原性脳病巣の病態病理2018

    • Author(s)
      柿田明美
    • Organizer
      第3回 東海生理懇談会/名古屋
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] てんかん原性脳病巣の病態病理:臨床に役立つ知見2018

    • Author(s)
      柿田明美
    • Organizer
      第59回 京滋奈良てんかん懇話会/京都
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 海馬硬化症における海馬支脚のてんかん原性.2017

    • Author(s)
      北浦弘樹、白水洋史、福多真史、増田浩、高橋均、柿田明美.
    • Organizer
      第58回日本神経病理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TBCD mutations cause autosomal recessive inherited early childhood-onset neurodegenerative encephalopathy.2017

    • Author(s)
      Miyake N, Chihara T, Miura M, Shizmizu H, Kakita A, Matsumoto N.
    • Organizer
      European Society of Human Genetics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multi-institutional study of epilepsy and glia in patients with medication-resistant focal epilepsy.2017

    • Author(s)
      Maehara T, Ina M, Hashimoto S, Kakita A, Ikeda A.
    • Organizer
      The 10th Epilepsy Colloquium: Miami, USA
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] グリア細胞のてんかん原性:病態病理学的解析. シンポジウム:グリアとてんかん:基礎と臨床のアップデート・インフォメーション.2017

    • Author(s)
      北浦弘樹、福多真史、藤井幸彦、柿田明美.
    • Organizer
      第51回 日本てんかん学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 基礎と臨床の包括的研究の重要性. シンポジウム:グリアとてんかん:基礎と臨床のアップデート・インフォメーション.2017

    • Author(s)
      前原健寿、橋本聡華、清水一秀、稲次基希, 池田昭夫, 柿田明美, 井上有史, 渡辺裕貴, 白水洋史, 福田敦夫, 小泉修一, 岡田元宏, 大野行弘
    • Organizer
      第51回 日本てんかん学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 発作時DC電位とその病理組織学的検討.2017

    • Author(s)
      小林環、北浦弘樹、村井智彦、中谷光良、菊池隆幸、人見健文、井内盛遠、松本理器、國枝武治、宮本享、白水洋史、井上有史、前原健寿、池田昭夫、柿田明美.
    • Organizer
      第51回 日本てんかん学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Clinical features and the pathomechanism of early childhood-onset neurodegenerative encephalopathy arising from biallelic TBCD mutations.2017

    • Author(s)
      Miyake N, Chihara T, Miura M, Shimizu H, Kakita A, Matsumoto N.
    • Organizer
      American Society of Human Genetics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spike onset zone detected by gradient magnetic-field topography (GMFT) reflects the epileptogenic zone in neocortical focal cortical dysplasia.2017

    • Author(s)
      Shirozu H, Hashizume A, Masuda H, Kakita A, Otsubo H, Kameyama S.
    • Organizer
      American Epilepsy Society Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] てんかん原性脳病巣の病態病理:臨床に役立つ知見2017

    • Author(s)
      柿田明美
    • Organizer
      熊本神経病理研究会/熊本
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] てんかん原性脳病巣の病態病理:臨床に役立つ知見2017

    • Author(s)
      柿田明美
    • Organizer
      Epilepsy forum in Nagaoka/長岡
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Alterations of glial cells in epileptogenic regions of the brain: a pathophysiological study.2017

    • Author(s)
      Kakita A, Kitaura H.
    • Organizer
      第60回日本神経化学会/仙台
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Histopathologic features indicating possible pathomechanisms underlying neurological disorders of infants and children.2017

    • Author(s)
      Kakita A
    • Organizer
      AOCCN: Asian Oceanian Congress of Child Neurology
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ヒトてんかん原性脳病巣の病態を知るための病理学的アプローチ.2016

    • Author(s)
      柿田明美.
    • Organizer
      第50回日本てんかん学会
    • Place of Presentation
      グランシップ(静岡県静岡市)
    • Year and Date
      2016-10-07
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 内側側頭葉てんかん患者における海馬硬化症と扁桃体の細胞脱落についての検討.2016

    • Author(s)
      中山遥子, 福多真史, 増田浩, 白水洋史, 伊藤陽祐, 東島威史, 北浦弘樹, 柿田明美, 藤井幸彦.
    • Organizer
      第57回日本神経病理学会
    • Place of Presentation
      ホテルニューキャッスル(青森県弘前市)
    • Year and Date
      2016-06-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 内側側頭葉てんかん患者の海馬硬化における酸化損傷タンパク質の同定とタンパク質発現変動解析.2016

    • Author(s)
      古川絢子、柿田明美、千葉陽一、樋口善博、亀山茂樹、島田厚良.
    • Organizer
      第57回日本神経病理学会
    • Place of Presentation
      ホテルニューキャッスル(青森県弘前市)
    • Year and Date
      2016-06-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 海綿状血管腫におけるヘモジデリン沈着とてんかん原性の検討.2016

    • Author(s)
      北浦弘樹、清水宏、福多真史、藤井幸彦、高橋均、柿田明美.
    • Organizer
      第57回日本神経病理学会
    • Place of Presentation
      ホテルニューキャッスル(青森県弘前市)
    • Year and Date
      2016-06-01
    • Related Report
      2016 Research-status Report
  • [Presentation] アストロサイト:病態における形態変化.2016

    • Author(s)
      柿田明美、豊島靖子、高橋均.
    • Organizer
      第57回日本神経病理学会
    • Place of Presentation
      ホテルニューキャッスル(青森県弘前市)
    • Year and Date
      2016-06-01
    • Related Report
      2016 Research-status Report
    • Invited
  • [Book] 稀少てんかんの診療指針.日本てんかん学会(編集).2017

    • Author(s)
      柿田明美(共著)
    • Total Pages
      259
    • Publisher
      診断と治療社
    • Related Report
      2017 Annual Research Report
  • [Book] てんかん学用語事典.日本てんかん学会(編集).2017

    • Author(s)
      柿田明美(共著)
    • Total Pages
      165
    • Publisher
      診断と治療社
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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