| Project/Area Number |
16K14598
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Kagoshima University |
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Principal Investigator |
OZAWA Masayuki 鹿児島大学, 医歯学域医学系, 教授 (90136854)
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 正宏 鹿児島大学, 医用ミニブタ・先端医療開発研究センター, 教授 (30287099)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 細胞接着 / カドヘリン / Cre-loxP系 / トランスジェニックマウス / 病態モデル / 細胞質ドメイン / 赤色螢光たんぱく質 / キメラ / 赤色蛍光タンパク質 / 細胞間接着 |
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| Outline of Final Research Achievements |
Expression of a chimeric molecule (DECT) composed of a red fluorescent protein (DsRed) and the cytoplasmic domain of E-cadherin (ECT) inhibits the cell surface transport of endogenous cadherins. Thus, establishment of mouse in which DECT expression can be induced time- and space-specific manner provides an animal model for diseases induced by deficiency in cadherin-mediated cell adhesion. We constructed an expression vector that expresses the lacZ reporter gene before Cre-mediated excision and expresses DECT following Cre excision, which removes the lacZ gene. This vector was introduced into ES cells by transfection. The lacZ gene was flanked by loxP sites. The DECT-coding sequence followed the loxP-flanked region. DECT was expected to not be expressed until after Cre excision of β-geo. These clones were found to be positive for β-galactosidase activity and negative for DsRed fluorescence. These ES cells will be used to establish transgenic mouse.
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