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gene introduction using myeloid lineage cells

Research Project

Project/Area Number 16K14634
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor therapeutics
Research InstitutionTeikyo University

Principal Investigator

Shirafuji Naoki  帝京大学, 医学部, 教授 (00206301)

Research Collaborator Tashiro Haruko  帝京大学, 医学部, 講師
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsCD34 / CD47 / 造血幹細胞 / 遺伝子導入 / CAR-T / PBSCT / G-CSF / CLL-1 / CD19 / 遺伝子運搬 / 好中球 / 抗腫瘍効果 / 骨髄芽球
Outline of Final Research Achievements

We constructed shuttle vector that expressed antisense nad miRNA RNA for human CD47. This vector was introduced into CD13 and CD33-positive bone marrow mononuclear cell fractions which was isolated with magnetic beads. The positive cell-fraction was cultured with recombinant human granulocyte colony-stimulating factor. The obtained mature neutrophilic granulocytes expressed anti CD47 antisense RNA and miRNA. Biological antitumor effects were also elevated.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2018 2017 2016

All Journal Article (8 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (16 results) (of which Int'l Joint Research: 8 results)

  • [Journal Article] Direct Factor X sequestration by systemic amyloid lightchain amyloidosis2018

    • Author(s)
      Haruko Tashiro, Ryosuke Shirasaki, Masato Watanabe, Kazuo Kawasugi, Yoshihisa Takahashi, Naoki Shirafuji
    • Journal Title

      Clinical Case Reports

      Volume: 6(3): Issue: 3 Pages: 513-515

    • DOI

      10.1002/ccr3.1398

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Aggressive NK Cell Leukemia with Hemophagocytic Lymphohistiocytosis: A Case Report2017

    • Author(s)
      Kimiko Nogi, Haruko Tashiro, Kazuo Kawasugi, Mayumi Matsuzawa, Takeshi Osawa, Ryohei Toyooka, Taiji Furukawa, Naoki Shirafuji
    • Journal Title

      J Clin Lab Med

      Volume: 2.2 Issue: 2 Pages: 1-4

    • DOI

      10.16966/2572-9578.116

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Immunotherapy against cancer-related viruses2017

    • Author(s)
      Tashiro H, Brenner MK
    • Journal Title

      Cell Res.

      Volume: 27 Issue: 1 Pages: 59

    • DOI

      10.1038/cr.2016.153

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Vaccination targeting native receptors to enhance the function and proliferation of Chimeric Antigen Receptor (CAR)-modified T cells2017

    • Author(s)
      Tanaka M, Tashiro H, Omer B, Lapteva N, Ando J, Ngo M, Mehta B, Dotti G, Kinchington PR, Leen AM, Rossig C, Rooney CM.
    • Journal Title

      Clin Cancer Res.

      Volume: 23 Issue: 14 Pages: 3499

    • DOI

      10.1158/1078-0432.ccr-16-2138

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comprehensive Approach for Identifying the T Cell Subset Origin of CD3 and CD28 Antibody-Activated Chimeric Antigen Receptor-Modified T Cells. J Immunol. 2017 May 26. pii: 1601494. doi: 10.4049/jimmunol.1601494.2017

    • Author(s)
      Schmueck-Henneresse M, Omer B, Shum T, Tashiro H, Mamonkin M, Lapteva N, Sharma S, Rollins L, Dotti G, Reinke P, Volk HD, Rooney CM.
    • Journal Title

      J Immunol. pii: 1601494.

      Volume: 199(1) Issue: 1 Pages: 348-362

    • DOI

      10.4049/jimmunol.1601494

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] CAR T Cells Administered in Combination with Lymphodepletion and PD-1 Inhibition to Patients with Neuroblastoma2017

    • Author(s)
      Heczey A, Louis CU, Savoldo B, Dakhova O, Durett A, Grilley B, Liu H, Wu MF, Mei Z, Gee A, Mehta B, Zhang H, Mahmood N, Tashiro H, Heslop HE, Dotti G, Rooney CM, Brenner MK.
    • Journal Title

      Mol Ther.

      Volume: 25 Issue: 9 Pages: 2214

    • DOI

      10.1016/j.ymthe.2017.05.012

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Treatment of Acute Myeloid Leukemia with T Cells Expressing Chimeric Antigen Receptors Directed to C-type Lectin-like Molecule 12017

    • Author(s)
      Tashiro H, Sauer T, Shum T, Parikh K, Mamonkin M, Omer B, Rouce R, Lulla P, Rooney C, Gottschalk S, Brenner MK. Treatment of acute myeloid leukemia with T cells expressing chimeric antigen receptors directed to C-type lectin-like molecule 1.
    • Journal Title

      Mol Ther.

      Volume: 25 Issue: 9 Pages: 2202

    • DOI

      10.1016/j.ymthe.2017.05.024

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Elevated level of serum antinuclear antibody and hemophagocytic syndrome in Asian-variant intravascular large B-cell lymphoma: a case report with its diagnostic pitfalls.2016

    • Author(s)
      Kikuchi Y, Ota Y, Matsuo T, ShirafujiN, Kondo F, Uozaki H
    • Journal Title

      Int J Clin Exp Pathol.

      Volume: 9 Pages: 7739-7743

    • Related Report
      2016 Research-status Report
  • [Presentation] IL-1-βと電気刺激による成人ヒト皮膚線維芽細胞のhemogenic endotheliumへの変化に関する研究2018

    • Author(s)
      白藤 尚毅,松尾 琢二,住吉 立,田代 晴子
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 赤血球抗原情報検索システムの有用性と問題点について2017

    • Author(s)
      灰野夏澄、前島理恵子、藤原孝記、冨山秀和、金子 強、永友ひとみ、蟹井はるか、笠井英利、難波宏美、大曽根和子、松本謙介、白藤尚毅。
    • Organizer
      第65回日本輸血・細胞治療学会(6月、千葉)
    • Related Report
      2017 Annual Research Report
  • [Presentation] ABO不適合臍帯血移植後のドナー由来赤血球抗原量の解析2017

    • Author(s)
      蟹井はるか、藤原孝記、前島理恵子、冨山秀和、灰野夏澄、金子 強、永友ひとみ、笠井英利、難波宏美、大曽根和子、松本謙介、白藤尚毅。
    • Organizer
      第65回日本輸血・細胞治療学会(6月、千葉)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 好中球増多を伴った多発性骨髄腫の1例。2017

    • Author(s)
      芳賀、幸、松本謙介、住吉 立,松尾 琢二,山本 義,白崎良輔、田代晴子、大井 淳,白藤尚毅、滝川 一。
    • Organizer
      第633回日本内科学会関東地方会 (6月、東京)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 全身性アミロイドーシスによる高度の第X因子欠乏を来したB細胞性リンパ増殖性疾患2017

    • Author(s)
      田代晴子、住吉 立,松尾 琢二,山本 義,松本謙介、大井 淳,秋山 暢,川杉 和夫,白藤尚毅。
    • Organizer
      第7回日本血液学会関東甲信越地方会(7月、松本)
    • Related Report
      2017 Annual Research Report
  • [Presentation] FLT3-ITD mutation in MDS onset is associated with early transformation to AML2017

    • Author(s)
      Ritsu Sumiyoshi, Tadashi Yamamoto, Takuji Matsuo, Yoko Oka-Miura, Haruko Tashiro, Kensuke Matsumoto, Jun Ooi, Naoki Shirafuji.
    • Organizer
      78回日本血液学会総会(10月、横浜)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Low HDL is a significant poor prognostic factor in malignant lymphoma (ML)2017

    • Author(s)
      Takuji Matsuo, Ritsu Sumiyoshi, Tadashi Yamamoto, Haruko Tashiro, Kensuke Matsumoto, Jun Ooi, Nobu Akiyama, Kazuo Kawasugi, Naoki Shirafuji.
    • Organizer
      78回日本血液学会総会(10月、横浜)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Perkinson’s disease (PD) developed after Bortezomib (Bor)-containing chemotherapy for Mantle cell lymphoma (MCL)2017

    • Author(s)
      Yoko Miura, Takuji Matsuo, Ritsu Sumiyoshi, Tadashi Yamamoto, Haruko Tashiro, Kennsuke Matsumoto, Jun Ooi, Naoki Shirafuji.
    • Organizer
      78回日本血液学会総会(10月、横浜)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Primary Adrenal Lymphoma with Adrenal Insufficiency: Report of Three Cases and Review of Literature2017

    • Author(s)
      Masumi Ogawa, Naoki Edo, Takuji Matsuo, Tadashi Yamamoto, Satoshi Takahashi, Yamato Mashimo, Koji Morita, Yuko Sasajima, Hiroshi Uozaki, Fukuo Kondo, Yasunori Ota, Naoki Shirafuji, Hiroko Okinaga, Kazuhisa Tsukamoto, Toshio Ishikawa.
    • Organizer
      ENDO 2017 (April, Orlando, Florida USA)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Constitutive Signaling from an Engineered IL-7 Receptor Promotes Durable Tumor Elimination by Tumor Redirected T-Cells2017

    • Author(s)
      Thomas Shum, Bilal Omer, Haruko Tashiro, Robert Kruse, Dimitrios Wagner, Kathan Parikh, Zhongzhen Yi, Tim Sauer, Robin Parihar, Leonid Metelitsa, Stephen Gottschalk, Cliona Rooney
    • Organizer
      ASGCT 20th Annual Meeting (May, Washington DC, USA)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A Single CRISPR-Mediated Knockout as Novel Suicide Switch and Selection Tool for Gene-Modified T Cells2017

    • Author(s)
      Dimitrios L. Wagner, Haruko Tashiro, Thomas Shum, Debopriyo Halder, Michael C. Gundry, Lorenzo Brunetti, Ciaran M. Lee, Benjamin H. Shin, Tim Sauer, Maksim Mamonkin, Gang Bao, Margaret A. Goodell, Cliona M. Rooney
    • Organizer
      ASGCT 20th Annual Meeting (May, Washington DC, USA)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Low high-density lipoprotein cholesterol (HDL) is a significant poor prognostic factor in malignant lymphoma2017

    • Author(s)
      Takuji Matsuo, Haruko Tashiro, Ritsu Sumiyoshi, Tadashi Yamamoto, Yoko Oka-Miura, Kensuke Matsumoto, Jun Ooi, Naoki Shirafuji
    • Organizer
      59th ASH meeting (December, Atlanta, Georgia, USA,)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] FLT3-ITD mutation in MDS patients is associated with early transformation to AML2017

    • Author(s)
      Ritsu Sumiyoshi, Haruko Tashiro, Takuji Matsuo, Tadashi Yamamoto, Yoko Oka-Miura, Kensuke Matsumoto, Jun Ooi, Naoki Shirafuji.
    • Organizer
      59th ASH meeting (December, Atlanta, Georgia, USA,)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biological effect of IL-1-beta to HDF for a long-term culture2016

    • Author(s)
      Ritsu Sumiyoshi, Takuji Matsuo, Ryosuke Shirasaki, Yoko Oka-Miura, Tadashi Yamamoto, Kennsuke Matsumoto, Jun Ooi, Nobu Akiyama, Kazuo Kawasugi, Naoki Shirafuji.
    • Organizer
      77th JSH
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2016-10-13
    • Related Report
      2016 Research-status Report
  • [Presentation] Outcome of salvage chemotherapy against relapsed or refractory advanced non-Hodgkin’s lymphoma cases2016

    • Author(s)
      Takuji Matsuo, Ritsu Sumiyoshi, Tadashi Yamamoto, Kennsuke Matsumoto, Jun Ooi, Nobu Akiyama, Kazuo Kawasugi, Naoki Shirafuji.
    • Organizer
      77th JSH
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2016-10-13
    • Related Report
      2016 Research-status Report
  • [Presentation] Vascular endothelial cell-relating molecules expressed in an adult human dermal fibroblast when cultured with interleukin-1-beta2016

    • Author(s)
      Rhitsu Sumiyoshi, Takuji Matsuo, Tadashi Yamamoto, Kennsuke Matsumoto, Jun Ooi, Kazuo Kawasugi, Naoki Shirafuji.
    • Organizer
      EHA
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2016-06-09
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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