Detection of monoallelically expressed genes under epigenetic control and quest for intraindividual genetic diversities
Project/Area Number |
16K14644
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Genome biology
|
Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Abe Kuniya 国立研究開発法人理化学研究所, バイオリソース研究センター, チームリーダー (40240915)
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | モノアレル発現遺伝子 / X染色体不活性化 / 刷り込み遺伝子 / 遺伝的多様性 / 多能性幹細胞 / モノアレル遺伝子発現 / 遺伝的モザイシズム / エピジェネティクス / 幹細胞 / エピブラスト幹細胞 / シングルセル解析 |
Outline of Final Research Achievements |
Monoallelically expressed genes (MEGs), both random and imprinted MEGs, could influence genetic constitution of individuals. However, nature of these MEGs and their regulations are not fully understood. In this study, we analyzed expression dynamics of MEGs during differentiation of embryonic stem cells by single cell RNA-Seq. We could analyze and delineate expression dynamics of X-linked random MEGs during progression of X chromosome inactivation process for the first time. We unexpectedly revealed that there are significant number of MEGs expressed in genetic-origin-dependent manner.
|
Academic Significance and Societal Importance of the Research Achievements |
本研究から得られたシングルセルRNA-Seqデータおよびこの度考案したアレル特異的発現解析パイプラインを用いることで、今回明らかにしたX染色体不活性化におけるモノアレル発現ダイナミクスに係わる知見のみならず、刷り込み型や常染色体上のランダムモノアレル発現遺伝子に関する研究にも貢献できると考えられ、本研究で得られた手法やデータは高い学術的価値を持つものと思われる。また、今回、初めて明らかになったgenetic-origin-dependent モノアレル発現遺伝子も、遺伝的多様性の創出に影響すると考えられ今後さらに追究されるべきと考えられる。
|
Report
(4 results)
Research Products
(43 results)
-
-
-
[Journal Article] WNT inhibition and increased FGF signaling promotes derivation of less heterogeneous primed human embryonic stem cells, compatible with differentiation2019
Author(s)
Taelman J, Popovic M, Bialecka M, Tilleman L, Warrier S, Van Der Jeught M, Menten B, Deforce D, Sutter P DE, Nieuwerburgh V, Abe K, Heindryckx B, Chuva de Sausa Lopes SM
-
Journal Title
Stem Cells and Development
Volume: in press
Issue: 9
Pages: 579-592
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
[Journal Article] SCPortalen: human and mouse single-cell centric database2017
Author(s)
Abugessaisa Imad、Noguchi Shuhei、Bottcher Michael、Hasegawa Akira、Kouno Tsukasa、Kato Sachi、Tada Yuhki、Ura Hiroki、Abe Kuniya、Shin Jay W、Plessy Charles、Carninci Piero、Kasukawa Takeya
-
Journal Title
Nucleic Acids Research
Volume: 46
Issue: D1
Pages: D781-D787
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Conserved role of Ovo in germline development in mouse and Drosophila.2017
Author(s)
Hayashi, M., Shinozuka, Y., Shigenobu, S., Sato, M., Sugimoto, M., Ito, S., Abe, K., Kobayashi, S.
-
Journal Title
Scientific Report
Volume: 6
Issue: 1
Pages: 40056-40056
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene2017
Author(s)
Michiko Hirose, Ayumi Hasegawa, Keiji Mochida, Shogo Matoba, Yuki Hatanaka, Kimiko Inoue, Tatsuhiko Goto, Hideki Kaneda, Ikuko Yamada, Tamio Furuse, Kuniya Abe, Yoshihisa Uenoyama, Hiroko Tsukamura, Shigeharu Wakana, Arata Honda, and Atsuo Ogura
-
Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 42476-42476
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Cellular dynamics of mouse trophoblast stem cells: Identification of a persistent stem cell type.2016
Author(s)
Motomura K, Oikawa M, Hirose M, Honda A, Togayachi S, Miyoshi H, Ohinata Y, Sugimoto M, Abe K, Inoue K, Ogura A.
-
Journal Title
Biology of Reproduction
Volume: 94
Issue: 6
Pages: 122-122
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-