| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Using the fungal capping enzymes Ceg1, Cet1 and Cet1 mutants that can not bind to Ceg1, we show that nuclear translocation signals are present only in Cet1, and that Ceg1 can not translocate to the nucleus without binding to Cet1. Furthermore, we demonstrate that Cet1 and Ceg1 bind in the cytoplasm, and that the Cet1-Ceg1 complex translocates to the nucleus. Moreover, genetic studies using yeast show that Cet1 and Ceg1 binding is essential for fungal growth. These data indicate that inhibition of Cet1-Ceg1 binding may be the mechanism of action of novel antifungal agents. Then, we obtained the aptamers that selectively inhibit the capping reaction of budding yeast that has been used as a model so far.
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