| Project/Area Number |
16K14659
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
System genome science
|
|---|
| Research Institution | Fujita Health University |
|---|
Principal Investigator |
Mayeda Akila 藤田保健衛生大学, 総合医科学研究所, 教授 (50212204)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YOSHIMOTO Rei 藤田保健衛生大学, 総合医科学研究所 遺伝子発現機構学研究部門, 助教 (70595652)
OHNO Mutsuhito 京都大学, 医学部 ウイルス再生医科学研究所, 教授 (80201979)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 遺伝子発現調節 / ノンコーディングRNA / 環状RNA / スプライシング / ciRS-7 / MIR / 非翻訳RNA |
|---|
| Outline of Final Research Achievements |
The circRNAs are abundant and stable noncoding RNAs with closed circular structure. The function of ciRS-7 (CDR1as) as ‘sponges’, or decoys, of the miR-7 micro RNA shed light on the biological roles of circRNAs.
We elucidated the biosynthesis pathway of this functional ciRS-7. Anti-sense oligoribonucleotide experiments targeting the relevant splice sites suggested the generation of ciRS-7 by back-splicing. We identified conserved inverted elements of MIR (mammalian-wide interspersed repeat) in the flanking region of the ciRS-7, which promoted the back-splicing event. Splicing reporter assays in stably transfected HEK293 cells with mini-genes, with and without MIR element, demonstrated the essential role of inverted MIR sequences in the ciRS-7 generation.
Previously the inverted repeats of Alu, primate-specific SINE was reported to involve in the back-splicing. The MIR-dependent circRNAs have emerged as a new category of circRNAs that are widely conserved in mammalian.
|
